Effect of auxin precursors and chemical analogues on the growth and chemical composition in Chlorella pyrenoidosa Chick

Abstract

In this paper the authors present studies on the effect of auxin precursors and chemical analogues on the growth and biochemical composition in <i>Chlorella pyrenoidosa</i> (<i>Chlorophyceae</i>). Among auxin precursors tryptamine exhibited slightly higher stimulative activity in regard to fresh and dry weight, mineral substances, chlorophylls, carotenoids, monosaccharides (aldohexoses) and water-soluble proteins content in <i>Ch. pyrenoidosa</i> cells as compared to anthranilic acid. Among auxin analogues used phenoxyacetic acid and naphthyl-3-acetic acid had the strongest stimulative effect of the above-mentioned parameters. Their activity was significantly higher than that of auxin precursors. The activity of naphthyl-3-sulphonic acid was slightly lower than that of tryptamine, whereas the stimulation by 2,4-dichlorophenoxyacetic acid was similar to that of anthranilic acid. In <i>Ch. pyrenoidosa</i> cells 2,4-dichlorophenoxyacetic acid and naphthyl-3-sulphonic reached their maximum activity at the latest (between the 15th or 16th day) of the culturing, whereas tryptamine, phenylacetic acid, naphthyl-3-acetic acid and indolyl-3-acetic acid - at the earliest (between the 8th or 12th) day

The effect of natural and synthetic auxins on the growth, metabolite content and antioxidant response of green alga Chlorella vulgaris (Trebouxiophyceae)

“…Our results confirm previous studies indicating that among auxins, IAA has the highest biological activity in algal cultures (Czerpak et al 1994). The activity of specific auxins is influenced by the position of the carboxyl group in the aliphatic chain.…”

“…The observed increases in protein, chlorophyll a, carotenoid and monosaccharide content due to the effects of exogenously applied auxins added to the growth media would be of value in the cultivation of C. vulgaris for commercial production of animal feed or bioproducts. Increased metabolite production has also been widely observed in C. pyrenoidosa due to exogenously applied auxins (Czerpak et al 1994;Czerpak and Bajguz 1997), auxin precursors and analogues (Czerpak et al 1994). The application of IAA, indole-3-lactic acid (ILA) and IBA to the growth media of C. pyrenoidosa increased the content of chlorophyll, carotenoid, aldohexoses and water-soluble proteins, with the strongest effect observed for IAA (Czerpak et al 1994).…”

“…Increased metabolite production has also been widely observed in C. pyrenoidosa due to exogenously applied auxins (Czerpak et al 1994;Czerpak and Bajguz 1997), auxin precursors and analogues (Czerpak et al 1994). The application of IAA, indole-3-lactic acid (ILA) and IBA to the growth media of C. pyrenoidosa increased the content of chlorophyll, carotenoid, aldohexoses and water-soluble proteins, with the strongest effect observed for IAA (Czerpak et al 1994). Natural (IAA, IBA, PAA) and synthetic (NAA) auxins were also found to have stimulatory effects on C. vulgaris growth and metabolite production in the present study.…”

Control of cytokinin and auxin homeostasis in cyanobacteria and algae

“…Detection of eight more unidentified metabolites in both cells and medium may indicate the existence of other, probably unknown IAA metabolic pathway(s) in green algae (Figs 4 and 5). Surprisingly, exogenous application of [ 3 H]NAA or [ 3 H]2,4-D did not have any significant effect on substrate metabolization (data not shown), although both of these synthetic auxins were shown to stimulate cell growth and to increase cell fresh and dry weight in Chlorella pyrenoidosa cultures (Czerpak et al , 1994). It may be speculated that the absence of metabolization of [ 3 H]NAA or [ 3 H]2,4-D is due to non-functional transport mechanisms or absent metabolic pathways for rapid inactivation of these unnatural auxins.…”

Phytohormones and Effects on Growth and Metabolites of Microalgae: A Review

“…Auxins can also enhance, in addition to growth and lipids, the biosynthesis of pigments, monosaccharides, and soluble proteins in C. vulgaris [20]. Increases of 213-273% for chlorophyll a and b content and of 164-258% for carotenoid content of C. pyrenoidosa were also found with seven precursors and analogs of auxins supplemented [24].…”