Effect of Auxin Type and Concentrations in Different Media on the Callus Induction and Shoot Formation of Crested Wheatgrass (Agropyron Cristatum(L.) Gaertn)

Can, Ersin; Çeli̇ktaş, Nafi̇z; Hatipoğlu, Rüştü

doi:10.1080/13102818.2008.10817553

Cited by 14 publications

(9 citation statements)

References 22 publications

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“…Among different concentrations of auxins (IBA, NAA and 2,4-D) and cytokinins (BAP and Kin), it was found that by increasing the concentration of growth regulators, there is a great fall in the callusing percentages which agrees with the report of Can et al (2008).…”

Section: Callus Induction Percentagesupporting

confidence: 85%

“…Kin proved less effective for shoot regeneration and gave comparatively lower regeneration percentages then BAP. Moreover Can et al (2008) observed the inefficiency of Kin over BAP for obtaining better regeneration percentages. A decreasing trend in regeneration percentages with increasing concentrations of BAP and Kin after an optimum level (3.0 mg L -1 ) was found among different treatments.…”

Section: Shoot Regeneration Percentagementioning

confidence: 98%

“…This acidification of the cell wall region activates wall-loosening proteins known as expansions to allow slippage of cellulose microfibrils in the cell wall and breaking of bonds in wall polysaccharides, allowing the walls to stretch more easily and to increase the callus growth more rapidly. Can et al (2008) reported that auxins make nuclear DNA to become more methylated than usual state which revert the cell to dedifferentiated state and initiate cell division and enlargement. In the present work, compact callus was observed on all concentrations of BAP which according to Suzuki et al (2002) was characterized with dense cytoplasm and conspicuous nuclei with comparatively thicker cell walls which were tightly packed together giving rise to an undifferentiated mass.…”

Section: Callus Growth and Types Of Callusmentioning

confidence: 99%

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In vitro regeneration of Gerbera jamesonii cv. Sunglow

Shabbir¹

2012

Afr. J. Biotechnol.

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This study demonstrates a protocol for in vitro regeneration of Gerbera jamesonii cv. 'Sunglow' developed by culturing leaf segments on Murashige and Skoog (MS) medium supplemented with 1.0, 1.5, 2.0, 2.5 and 3.0 mg L-1 indole-3-butyric acid (IBA), naphthalene acetic acid (NAA), 2,4dichlorophenoxy acid (2,4-D) and 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1 benzyl adenine (BAP) and kinetin (Kin). The treatment, 1.5 mg l-1 2,4-D gave 100% callus induction with a friable, nodular and creamish white callus. BAP gave fair callus growth with compact and brownish callus. However, Kin failed to produce callus. Shoot regeneration was assayed with 1.0, 2.0, 3.0, 4.0 and 5.0 mg L-1 BAP and Kin. BAP at 3.0 mg l-1 showed 76.67% shoot regeneration, 4 shoots per explant and shoot length of 9 cm, whereas same concentration of Kin gave only 16.67% regenerated shoots, 1.97 shoots per explant and length of 5.167 cm. In vitro root induction was determined by using 0.5, 1.0, 1.5 and 2.0 mg L-1 indole-3-acetic acid (IAA) and NAA. IAA at 1.5 mg L-1 exhibited a rooting percentage of 97.67%, whereas similar concentration of NAA gave relatively less rooting percentage of 60.67%. IAA gave thick roots with maximum root number (7.567/per explant) and length (7.33 cm), on the contrary, NAA gave relatively thin roots having less number of 3.567 roots with a length of 4.667 cm.

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Section: Callus Induction Percentagesupporting

confidence: 85%

Section: Shoot Regeneration Percentagementioning

confidence: 98%

Section: Callus Growth and Types Of Callusmentioning

confidence: 99%

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In vitro regeneration of Gerbera jamesonii cv. Sunglow

Shabbir¹

2012

Afr. J. Biotechnol.

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“…King's Ruby. These findings are supported by Can et al (2008), who found that higher concentrations of auxins and cytokinins inhibited meristematic cell division and callus induction and decreased chemical reactions, resulting in the stunted growth and finally the death of explants.…”

Section: Callus Inductionmentioning

confidence: 56%

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Khan¹,

Ahmed

Hafiz

et al. 2015

OENO One

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Aim: To optimize the concentrations of growth regulators in the media for the proficient micropropagation of grapevine (Vitis vinifera L.) cv. King’s Ruby.Methods and results: Apical meristems of the grape cultivar were used to establish in vitro shoot cultures. Nodal explants, each containing an axillary bud, taken from in vitro grown shoots were inoculated in shoot proliferation medium, i.e., half strength Murashige and Skoog (MS) medium supplemented with benzyl aminopurine (BAP), kinetin, glycine and gibberellic acid (GA3). A higher number of shoots (5.33) with greater shoot length (2.75 cm) was produced in the medium supplemented with 1.0 mg L-1 BAP and 0.1 mg L-1 GA3. Calluses were induced from leaf explants taken from in vitro grown shoots. Callus induction was greater (73.00%) on the medium containing 2.0 mg L-1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.3 mg L-1 BAP and 0.2 mg L-1 α-naphthaleneacetic acid (NAA). The maximum frequency of shoot regeneration (53.33%) was achieved on the medium supplemented with 1.5 mg L-1 BAP and 0.5 mg L-1 NAA, and the regenerated shoots successfully formed roots on growth regulator-free half strength MS medium.Conclusion: Optimizing the concentration of BAP and GA3 and omitting the glycine and kinetin in the culture medium increased the number and length of shoots. Similarly, for inducing the callus of the leaf explants, taken from in vitro grown shoots, it is recommended to adjust the medium with the higher concentration of 2,4-D and lower concentrations of BAP. Moreover, the maximum number of shoots was regenerated on a medium supplemented with relatively high levels of both BAP and NAA (1.5 and 0.5 mg L-1, respectively). Finally, we suggest the half strength MS medium that is free from growth regulators for the root formation of the regenerated shoots.Significance and impact of the study: Optimizing the concentration of growth regulators is crucial for the efficient micropropagation of a grape cultivar. Knowing the specific balance between the growth regulators is necessary to establish in vitro shoot cultures, callus induction and shoot regeneration and, hence, to propagate disease-free true to type grape cultivars in a short time.

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“…Although vetiver callus induction usually prefers a high ratio of auxin to cytokinin, an optimal combination of auxin and cytokinin is still needed to be determined for each kind of explant. Supplementing auxin in CIM is essential for inducing division and development of callus cells into embryogenic cells, especially for monocotyledon plants (Mendoza and Kaeppler, 2002;Shah et al, 2003;Can et al, 2008;Neibaur et al, 2008). However, a high concentration of auxin in a medium may prevent cell differentiation (Gray, 1996).…”

Section: Effects Of Pgrs In Cim On Callus Induction and Plant Regenermentioning

confidence: 99%

Synergistic effects of BAP and kinetin media additives on regeneration of vetiver grass (Vetiveria zizanioides L. Nash)

Sompornpailin¹,

Khunchuay²

2016

Aust J Crop Sci

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Regeneration efficiency was enhanced by adding auxin and cytokinin at different concentrations to the media. It was found that the two highest frequencies-90 and 85%-of callus induction occured when the buds were cultured in an MS medium supplemented with 2 mgL-1 α-naphthalene acetic acid (NAA) plus 1 mgL-1 N 6-benzylaminopurine (BAP) (CIM2) and in the same medium supplemented with 2 mgL-1 NAA plus 1 mgL-1 kinetin (CIM5), respectively. When cultured further in a plant regeneration medium supplemented with 1 mgL-1 NAA and 2 mgL-1 BAP (PRM1), these calli regenerated with high efficiencies, 80% for calli grown in CIM2 and 85% for calli grown in CIM5. The low BAP concentration in CIM1 (2 mgL-1 NAA and 0.5 mgL-1 BAP) resulted in a low regeneration efficiency and fewer shoots per callus even though the PRM3 regeneration medium had a high concentration of cytokinins (2 mgL-1 BAP and 2 mgL-1 kinetin). However, the calli cultured in CIM2 without kinetin but with a suitable level of BAP still regenerated with high efficiencies in PRM2 and PRM3 that contained 1 and 2 mgL-1 kinetin, respectively. Similarly, when calli cultured in CIM5 without BAP but with kinetin were regenerated in PRM1 with BAP alone, they also regenerated with a high regeneration efficiency. These results showed that culturing a specific tissue in a medium with suitable concentrations of NAA and BAP or kinetin resulted in a large number of multiple shoots, and BAP and kinetin worked synergistically to produce a high plant regeneration efficiency.

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Effect of Auxin Type and Concentrations in Different Media on the Callus Induction and Shoot Formation of Crested Wheatgrass (Agropyron Cristatum(L.) Gaertn)

Cited by 14 publications

References 22 publications

In vitro regeneration of Gerbera jamesonii cv. Sunglow

In vitro regeneration of Gerbera jamesonii cv. Sunglow

Optimizing the concentrations of plant growth regulators for in vitro shoot cultures, callus induction and shoot regeneration from calluses of grapes

Synergistic effects of BAP and kinetin media additives on regeneration of vetiver grass (Vetiveria zizanioides L. Nash)

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