Effect of auxins on Karwinskia humboldtiana root cultures

Kollárová, Karin; Lišková, Desana; Kákoniová, D.; Lee, Alexander

doi:10.1007/s11240-004-0662-z

Cited by 22 publications

(13 citation statements)

References 19 publications

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“…Lateral roots are preferred in the production of secondary metabolites (Murthy et al, 2008). A high concentration of NAA induced callus-like and fragile roots not only in E. campestre but in some other species: Aloe vera, E. maritimum, and Karwinskia humboldtiana (Kollarova et al, 2004;Lee et al, 2011;Kikowska et al, 2014). In the present study, the supplementation with IAA and NAA was superior over IBA in initiation of adventitious roots and produced the maximum biomass of roots.…”

Section: Disscusionmentioning

confidence: 99%

Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity

Kikowska¹,

Thiem²,

Śliwińska³

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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An efficient micropropagation protocol for production of genetically uniform clones of Eryngium campestre L. was developed. To determine the effect of nutritional and hormonal factors on shoot and root development and bioactive compounds production, three variants of media differing in the content of macro-and micronutrients, as well as plant growth regulators of various types and concentrations were tested. The highest regeneration (100%), with over 13 shoots per explant, was induced on Murashige and Skoog (MS) medium with 1.0 mg l -1 benzyladenine (BA) and 0.1 mg l -1 indole-3-acetic acid (IAA). The in vitro derived shoots multiplied through axillary bud formation were rooted and transferred to an experimental plot with 78% frequency of survival. Flow cytometry showed no variation in nuclear DNA between the seedlings and micropropagated plants. Preliminary thin layer chromatography (TLC) analysis indicated that phenolic acids, saponins, flavonoids and acetylenes were present in plant biomass. Ultra high performance liquid chromatography (UHPLC) analysis revealed that shoots and roots from in vitro derived plants and root cultures maintained the ability to produce rosmarinic acid (RA), rosmarinic acid hexoside (RA-HEX) and chlorogenic acid (CGA). The highest phenolic acid content was detected in roots of in vitro regenerated plants. The extract from those roots expressed the highest inhibitory effect against bacteria Staphylococcus aureus, as well as dermatophytes Trichophyton mentagrophytes and T. rubrum.

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Section: Disscusionmentioning

confidence: 99%

Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity

Kikowska¹,

Thiem²,

Śliwińska³

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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“…Primary and lateral root growth was promoted by IBA, whereas NAA strongly inhibited rooting of a Chinese medicinal plant, Ohwi (P. lobata) [22], and Karwinskia (K. humboldtiana) [23]. The superior effects of IBA on root formation by these plant species, including poplar, might be due to several factors, such as its preferential uptake, transport, and stability over NAA and subsequent gene activation [24].…”

Section: Discussionmentioning

confidence: 99%

Direct Regeneration of Plants Derived from in vitro Cultured Shoot Tips and Leaves of Poplar (Populus × euramericana ‘Neva’)

Jiang¹,

Liu²,

Zheng³

2015

JLS

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Abstract:The purpose of the present study was to establish a regeneration procedure for Populus × euramericana 'Neva' by using in vitro shoots tips and leaves. For sterilization, 0.1% (w/v) mercuric chloride (HgCl 2 ) solution for 8 to 10 min was the optimal treatment for this poplar cultivation. The effects of benzyladenine (BA) and α-naphthaleneacetic acid (NAA) added to Murashige and Skoog (MS) medium were tested on organogenesis. The highest regeneration rate and numbers of shoots/explant from shoot tips (96.7%, 9.8) and leaves (90.0%, 8.7) were obtained on the half-strength MS medium supplemented with 0.5 mg/L BA and 0.1 mg/L NAA. The optimal medium for in vitro rooting of shoots was on a half-strength MS medium containing 1 mg/L indolebutyric acid (IBA) with the highest rooting frequency (93.3%) and numbers of roots/explant (8.2). For acclimatization, in vitro rooted plantlets were transferred to plastic cups containing vermiculite and peat (1: 1). After acclimatization, transplanted plantlets grew well in a shade house. Therefore, we believe that this efficient plant regeneration protocol especially by leaf explants is very important for in vitro clonal propagation of Populus × euramericana 'Neva'.

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“…These peculiarities have encouraged the development of several studies to evaluate the production of compounds by calluses with different characteristics, mainly concerning consistence and morphogenic potential (Flores & Nicoloso, 2007). For this, the establishment of efficient protocols determining the induction and maintenance of friable calluses is needed (Kollávorá, 2004;Oksman-Caldentey & Inzé, 2004).…”

Section: Introductionmentioning

confidence: 99%

Callogenesis in leaves of Kalanchoe pinnata Lam. by 2,4-D and BA action

Santos

Ferreira

Guimarães

et al. 2014

Rev. bras. plantas med.

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ABSTRACT:The Kalanchoe pinnata Lam. is a bush species of the Crassulaceae that is distinguished by its important medicinal properties. Its leaves are used as cataplasm to treat headaches and wounds. There is evidence for a hypotensive and anti-inflammatory effect. Techniques of plant tissue culture have been applied to plant species that produce substances likely to be explored in pharmacology, cell suspension being the main technique. At the industrial level, this method utilizes bioreactors in order to produce secondary metabolites on a large scale. The objective of this study was to evaluate the effects of in vitro combinations of 2,4-dichlorophenoxiacetic acid (2,4-D) and benzylaminopurine (BA) on callus induction in leaf explants of K. pinnata. Leaf fragments were inoculated in MS medium supplemented with 3.0% sucrose, 0.8% agar and factorial combinations of 2,4-D (0.00, 4.52, 9.06, 18.12 µM) and BA (0.00, 4.44, 8.88, 17.76 µM). The cultures were kept in the darkness at 24±2ºC for 50 days. The percentage of callus induction and the area of explants covered by callus cells were evaluated. In the absence of growth regulators, callus induction did not occur, with necrosis of all explants. The highest percentage of callus induction was 100%, obtained with the combination of 9.06 µM 2,4-D and 8.88 µM BA, but the calluses covered only 25% of the leaf area. The most efficient combination was 4.52 µM 2,4-D and 8.88 µM BA, resulting in 91% callus induction with 50 to 100% of the explants being covered by callus cells. Keywords:Medicinal plant, growth regulators, cell suspension. RESUMO: Calogênese em folhas de Kalanchoe pinnata Lam. pela ação de 2,4-D e BAP.Kalanchoe pinnata Lam. é uma espécie arbustiva da família Crassulaceae que apresenta interessantes propriedades medicinais. Suas folhas são utilizadas em cataplasma para tratar enxaqueca e ferimentos. Há evidência de seu efeito como hipotensiva e anti-inflamatória. Técnicas de cultura de tecidos vegetais têm sido aplicadas para espécies que possuem substâncias passíveis de exploração na farmacologia, sendo a suspensão celular a principal técnica utilizada. A nível industrial, este método utiliza biorreatores para produzir metabólitos secundários em larga escala. Este estudo teve como objetivo avaliar os efeitos in vitro de combinações do ácido 2,4-diclorofenoxiacético (2,4-D) e de benzilaminopurina (BAP) na indução de calos em explantes foliares de K. pinnata. Fragmentos foliares foram inoculados em meio MS contendo 3% de sacarose, 0,8% de ágar e combinações fatoriais de 2,4-D (0,00; 4,52; 9,06 e 18,12 µM) e BAP (0,00; 4,44; 8,88 e 17,76 µM). Os cultivos foram mantidos no escuro, a 24±2ºC por 50 dias. A porcentagem de indução de calos e a área dos explantes coberta por células de calos foram avaliadas. Na ausência de reguladores de crescimento não ocorreu indução de calos, com necrose de todos os explantes. A porcentagem mais alta de indução de calos foi de 100%, obtida com a combinação de 9,06 µM de 2,4-D e 8,88 µM de BAP, mas estes calos cobriram apenas 25% da...

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Effect of auxins on Karwinskia humboldtiana root cultures

Cited by 22 publications

References 19 publications

Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity

Micropropagation of Eryngium campestre L. via Shoot Culture Provides Valuable Uniform Plant Material with Enhanced Content of Phenolic Acids and Antimicrobial Activity

Direct Regeneration of Plants Derived from in vitro Cultured Shoot Tips and Leaves of Poplar (Populus × euramericana ‘Neva’)

Callogenesis in leaves of Kalanchoe pinnata Lam. by 2,4-D and BA action

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