Effect of Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus endospores on growth performance, nutrient digestibility, meat quality, relative organ weight, microbial shedding and excreta noxious gas emission in broilers

Abstract

ABSTRACT:This study was conducted to evaluate the effects of Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus (tri-strain probiotics, TSP) endospores in broilers. TSP can benefit the host animal by increasing nutrient absorption from the gastrointestinal tract and altering the intestinal ecosystem in poultry. A total of 500 day-old ROSS 308 mixed sex broiler chickens with an average initial body weight (IBW) of 45 g ± 0.6 g were used in this 35-day feeding study. Broiler chickens were randomly allotted to one of five dietary treatments: (1) CON (antibiotic free diet), (2) ANT1 (CON + enramycin 5 ppm), (3) ANT2 (CON + avilamycin 5 ppm), (4) TSP1 (CON + 0.1% TSP), and (5) TSP2 (CON + 0.2% TSP) with five replicates per treatment and 20 chicks per pen. Broiler chickens fed on TSP diets exhibited linearly increasing body weight gain (BWG) and decreased feed conversion ratio (FCR) compared to those on the CON diet (P < 0.05; Day 21 to Day 35 and Day 1 to Day 35, respectively). Further, dry matter (DM) and nitrogen (N) digestibility were improved (P < 0.05) in the TSP treatment at the end of study. The inclusion of TSP reduced (linear, P = 0.02) meat lightness (L*) compared with CON and ANT treatments. Broiler chickens fed with TSP diets had relatively higher (linear, P < 0.05) bursa weight than those fed with ANT and CON diets. The supplementation of TSP increased (P < 0.05) the ileal and caecal Lactobacillus count compared with CON and ANT diets. The ileal and caecal Escherichia coli and caecal Clostridium perfringens counts were reduced (P < 0.05) in the TSP2 group compared with the CON group. Broiler chickens fed with TSP diets exhibited increased (P < 0.05) caecal Bifidobacteria counts compared with CON and ANT diets. Excreta ammonia (NH 3 ) gas emission was lower (P < 0.05) with the TSP treatment compared with the CON treatment. In conclusion, the supplementation of TSP improved growth performance, nutrient digestibility, meat quality, gut health and reduced noxious gas emission in broilers.Keywords: ammonia gas emission; body weight gain; bursa weight; Escherichia coli; Lactobacillus; meat lightness List of abbreviations BWG = body weight gain, FCR = feed conversion ratio, DM = dry matter, L* = meat lightness, N = nitrogen, NH 3 = ammonia, TSP = tri-strain probiotics

<b>Effect of dietary probiotic supplementation on carcass traits and haematological responses of broiler chickens fed shea butter cake based diets

“…This is in agreement with the findings of Hossain, Begum, and Kim (2015) who reported anincrease in the bursa weight of broilers fed tri-strain probiotics compared to the control group. Besides, Willis, Isikhuemhen, and Ibrahim (2007) also suggested that the bursa was the primary lymphoid organ in broilers and concluded that probiotics could increase the relative weight of bursa to body weight.…”

Effect of dietary supplementation of Lactobacilli and Streptococci cultures on the performance of broiler chickens

“…Jin et al (1996) reported that dietary supplementation of 0.2% of Lactobacillus to male Arbor Acres broiler chicks from 10-day old to 38 days of growth significantly increased weight gain. Hossain et al, (2015) reported that broilers fed with Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus (tri-strain probiotics) endospores shown increased body weight gain.…”

“…In contrast, some other investigators showed that the FCR was significantly reduced when the broiler chicks were fed with 0.5% yoghurt for 42 days (Mansoub, 2011), 0.2% of Lactobacillus from day 10 to 38 days (Jin et al, 1996), and Bacillus subtilis, Clostridium butyricum and Lactobacillus acidophilus (Hossain et al, 2015). However, Samarakoon and Samarasinghe (2012) reported that there is a linear increase in FCR of broiler chicken with their age.…”

Inclusion of dietary multi-species probiotic on growth performance, nutrient digestibility, meat quality traits, faecal microbiota and diarrhoea score in growing–finishing pigs

“…In growing-finishing pigs, dietary probiotics can improve growth performance and meat quality (Alexopoulos et al 2004;Ceslovas et al 2005;Shon et al 2005;Davis et al 2008). It has been well accepted that dietary probiotics could benefit animal performance by producing antibacterial substances in their intestines by competing with harmful gut flora and stimulating the immune system (Fuller 1992;Hossain et al 2015;Lan et al 2017). Multi-species preparations have an advantage on growth performances in livestock animals when compared to mono-and multi-strain probiotics (Timmerman et al 2004).…”