Effect of Bacterial Resistance of Escherichia coli From Swine in Large-Scale Pig Farms in Beijing

Abstract

With widespread use of antibiotics in the aquaculture industry, bacterial resistance has recently attracted increasing attention. Continuous emergence of multi-resistant bacteria has greatly threatened human and animal health, as well as the quality and safety of livestock products. To control bacterial resistance, the effect of bacterial resistance needs to be well understood. The purpose of this study was to explore the factors influencing Escherichia coli (E. coli) drug resistance in large-scale pig farms. In this study, 296 strains of E. coli isolated and identified from large-scale pig farms in Beijing were used as the research objects. In vitro drug sensitivity tests were used to determine the sensitivity to 10 antibiotics of pig-derived E. coli. SPSS logistic regression was employed to analyze the effects of the season, pig type, sampling point (medication type) and sampling location on resistance and multi-drug resistance of E. coli from pigs. The degrees of drug resistance to 10 antibiotics of the 296 strains of pig-derived E. coli were varied, their resistance rates were between 4.05 and 97.64%, and their multi-drug resistance was appalling, with the highest resistance to six antibiotics being 26.35%. The isolated strains were proven more resistant to tetracyclines, penicillin and chloramphenicol, which are commonly used for disease prevention in pig farms, and less resistant to quinolones and aminoglycosides, which are not used in pig farms. The resistance of the isolated strains in spring and summer was generally higher than that in winter. E. coli resistance in piglets, fattening pigs and sows was more serious than that in nursery and sick pigs. The results showed that the season, type of medication and type of pig had an influence on the pig-derived E. coli resistance, among which the type of medication was the most influencing factor.

The role of short journey transportation in the spreading of swine pathogens and antimicrobial-resistant bacteria

“…Our results on the prevalence of AMR bacteria are consistent with ndings from previous studies carried at environmental level (33,34) or by faecal samplings (35)(36)(37). Our results highlight a relatively common occurrence of resistance against common antimicrobials (38), such as tetracycline, chloramphenicol, and trimethoprim/sulfamethoxazole, while resistance to last-resort antimicrobials, such as colistin, broadspectrum cephalosporins and carbapenems is less frequent.…”

The Diversity of Escherichia coli Pathotypes and Vaccination Strategies against This Versatile Bacterial Pathogen

“…The wide use of antibiotics has consequences, and there is a global concern in increasing antibiotic resistance. It has been reported that there is a high level of antibiotic resistance in porcine E. coli isolates from China [ 468 , 469 , 470 , 471 , 472 , 473 ]. Antibiotic resistance in E. coli is also prevalent on pork farms across Europe, including Spain [ 455 , 474 ], and other pork exporting countries such as Denmark, France, Italy, and Sweden are also found to be multi-drug resistant to antibiotics such as ampicillin, streptomycin, sulphonamide, tetracycline, and trimethoprim [ 475 ].…”

The Effect of Antimicrobial Peptide (PA-13) on Escherichia coli Carrying Antibiotic-Resistant Genes Isolated from Boar Semen

“…It is documented that most of the bacteria contaminated in fresh boar semen are E. coli [1-3,6], which show resistance to amoxicillin and ceftriaxone, whereas E. coli isolated from pig rectal and nasal swabs are resistant to tetracyclines, penicillin, and chloramphenicol [6,28]. Since antibiotic-resistant genes can spread to the surrounding environments of pig farms and also to humans, E. coli that is resistant to antibiotics on a pig farm remains a risk factor to public health and pig production [29].…”