Effect of Bavistin and Adenine Sulphate on In vitro Shoot Multiplication of Picrorhiza scrophulariiflora

Abstract

An alternative protocol for in vitro propagation of Picrorhiza scrophulariiflora is described using bavistin and adenine sulphate. The explants differentiated into multiple shoot buds on MS supplemented with various concentrations of bavistin and adenine sulphate ranging from 0 -400 mg/l either alone or in combination. Maximum number of multiple shoots were obtained on MS containing the combination of bavistin (100 mg/l) and adenine sulphate (100 mg/l). In this combination as high as 28 shoots per explant was achieved and also vetrification of the cultures were not recorded. This study also demonstrates that the bavistin has stronger cytokinin-like activity than adenine sulphate. For instance, it was observed that bavistin alone in the concentration of 300 mg/l produced as high as 24 shoots per explant, however, adenine sulphate (100 mg/l) could produce a maximum of 18 shoots per explant. Moreover, higher or lower concentration did not improve the shoot multiplication. The microshoots were separated from the multiple shoots and transferred to MS containing various concentrations of auxins. Among them, NAA (1 mg/l) produced as high as 6 roots per explant. The regenerated plantlets were hardened in plastic cups (6 x 8 cm) containing 9 : 1 virgin soil and soil at Kyongnosla nursery and acclimated for four weeks. A 90% survival rate of the plants was recorded after 60 days.

In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

“…Jia et al 1999). P. scrophulariiflora contains an additional glycosides which are absent in Picrorhiza kurroa (Li et al 1998, Smith et al 2000 and total picroside content is much higher than in P. kurroa (Bantawa et al 2009b). Conventional propagation methods through seed and root tubers for cultivation of this plant are too slow.…”

“…Our group have established a reproducible micropropagation protocols (Bantawa et al 2009b(Bantawa et al , 2010 and somatic embryogenesis (Bantawa 2009) of P. scrophulariiflora. However, there is no report on organogenesis of this plant, , which is the prerequisite for any genetic manipulation and for conservation of this endangered medicinal plant.…”

“…We used the in vitro leaves from previously established in vitro culture for producing callus (Bantawa et al 2009b). In present experiments, young 2-week-old in vitro leaves were inoculated on Woody plant medium (Lloyd and McCown 1980;WPM) with various concentration of 2,4-dichlorophenoxyacetic acid (2,4-D), α-napthaleneacetic acid (NAA), kinetin (KIN) or 6-benzylaminopurine (BAP), 3 % sucrose and 0.8 % Difco agar (M/S Himedia Laboratories, Mumbai, India).…”

Influence of Bavistin and Silver Thiosulphate on <i>in Vitro</i> Regeneration of <i>Asclepias curassavica</i> (L). Using Nodal Explants

“…The beneficial effects of ethylene inhibitors on organogenesis for plant regeneration have been widely reported [12][13][14]. The influence of Bavistin and silver thiosulphate has been well documented in many medicinal plants such as Mentha piperita [15], Stevia rebaudiana [16] and effect of Bavistin with adenine sulphate has been studied in Picrorhiza scrophulariiflora [17].…”

Influence of Bavistin, Cefotoxime, Kanamycin and Silver Thiosulphate on Plant Regeneration of <i>Solanum viarum</i> (Dunal)—An Important Anticancer Medicinal Plant

“…phulariiflora [8]. Since studies on in vitro plant regeneration of this species using different antimicrobial agents and ethylene inhibitor STS are almost limited, hence in the present study, we report a protocol on the effect of different concentrations of bavistin; cefotoxime and kanamycin along with STS were examined.…”