Effect of Bead Composition, PVS Type, and Recovery Medium in Cryopreservation of Bleeding Heart ‘Valentine’—Preliminary Study

Abstract

Bleeding heart (Lamprocapnos spectabilis (L.) Fukuhara) is a valuable ornamental and medicinal perennial. To date, there are few studies focused on cryopreservation of this species, although it could be useful in storage and breeding. This research is aimed at analyzing the effect of bead composition, type of plant vitrification solution (PVS), and the recovery medium of cryopreservation of bleeding heart. Shoot tips of L. spectabilis ‘Valentine’ were used in the study. The explants were precultured on modified Murashige and Skoog medium (MS; 1962), supplemented with 9% sucrose, 1.0-mg·L−1 kinetin (KIN), and 2.62-mg·L−1 abscisic acid. Next, in the first experiment, the shoot tips were embedded in 3% calcium alginate, based either on an MS medium or distilled sterile water. The produced synseeds were inoculated on the recovery medium with 3.0-mg·L−1 KIN, 0.5-mg·L−1 6-benzyladenine (BA), or cytokinin–free control. Based on the results of the first study, in the second experiment, precultured shoot tips were embedded in 3% calcium alginate based on MS medium and dehydrated with PVS2 or PVS3 for various durations. The pre-treated explants were plunged in liquid nitrogen and, after rewarming, inoculated on the recovery MS medium with 0.5-mg·L−1 BA. PVS3 was more effective in securing the shoot tips than PVS2. The highest recovery level (68.3%) was reported after a 150-min pretreatment with PVS3. Explants from this experimental combination also proliferated the highest number of shoots, as well as those with the greatest length. On the other hand, a higher share of dry weight was found in PVS2-derived shoots (13.5–18.2%) compared with plants produced after PVS3 treatment (10.6–11.4%). The obtained results here can serve as a good basis for further studies related to synthetic seeds and cryopreservation of bleeding heart.

Micropropagation and Cryopreservation of Yukon Draba (Draba yukonensis), a Special Concern Plant Species Endemic to Yukon Territory, Canada

“…However, contrasting results are observed in some species where the longer duration of PVS2 and PVS3 treatment are optimum. In Bleeding Heart (Lamprocapnos spectabilis), shoot tips recovered best after 150-min PVS3 treatment [98]. The shoot tips of Golden Paintbrush had a high regrowth at 100 min of treatment with concentrated PVS3 [19].…”

Efficient Protocol for Improving the Development of Cryopreserved Embryonic Axes of Chestnut (Castanea sativa Mill.) by Encapsulation–Vitrification

“…Synthetic seed technology, in addition to fulfilling needs related to micropropagation [ 35 , 36 , 37 , 38 , 39 ], can prove to be an efficient tool for the storage of rare and commercially important species at low temperatures. It has the potential for the medium-term and long-term preservation of plant explants encapsulated in synthetic seeds, without losing viability after immersion in LN when cryopreservation is applied [ 26 , 27 , 28 , 40 , 41 ].…”

Cryopreservation of Plant Tissues in Poland: Research Contributions, Current Status, and Applications

“…It was also reported that encapsulated shoot tips had a higher tolerance to prolonged dehydration than the "naked" explants, regardless of LN storage (Kulus, 2020b). Supplementing the alginate bead matrix with salts and vitamins from the MS medium and the effect of this factor on plant development were evident (Kulus, 2020a). Encapsulated explants produced longer shoots with larger leaves and higher fresh weights than untreated controls did (Kulus, 2020c).…”

“…Comparative analyses indicated that plant vitrification solution 3 (PVS3) (Nishizawa et al, 1993) was the most effective in the cryopreservation of L. spectabilis, both in terms of explant survival and the quality of the recovered plants (Kulus, 2020a).…”