Effect of Beauveria bassiana infection on the feeding performance and antioxidant defence of red palm weevil, Rhynchophorus ferrugineus

Hussain, Abid; Rizwan-ul-Haq, Muhammad; Al-Ayedh, Hassan; Ahmed, Sohail; AlJabr, Ahmed Mohammed

doi:10.1007/s10526-015-9682-3

Cited by 42 publications

(53 citation statements)

References 33 publications

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“…The present study revealed that all isolates exhibited more than 96% conidial viability (F = 0.36; df = 2, 12; P = 0.708), even from the least virulent studied isolate 7234. These findings are in agreement with previous studies indicating that low virulence among highly viable isolates of fungi reflects that virulence is regulated by multiple sets of traits (Hussain et al 2015 andHussain et al 2016). In the present study, significant differences (F = 101; df = 2, 12; P < 0.0001) in Pr1 activity in all of the studied EPF isolates were also recorded.…”

Section: Pathogen Virulencesupporting

confidence: 93%

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Host-pathogen interaction for screening potential of Metarhizium anisopliae isolates against the date-palm dust mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae)

Hussain

Rizwan-ul-Haq

AlJabr

et al. 2019

Egypt J Biol Pest Control

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Impact of virulence-determining traits of the fungus, Metarhizium anisopliae (Sensu lato), isolates 2049, 8762, and 7234, on mortality rate of the date-palm dust mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae), and host antioxidant defense-related enzymes were evaluated. Laboratory bioassays showed significant differences in the mortality rates of the mite species against fungal infection. Isolate 2049 (LT 50 = 6.43 days; LC 50 = 2.37 × 10 6 conidia/ml) was the most virulent one, revealing (160.64%) higher activity of the cuticle-degrading Protease (Pr1) than least virulent isolate 7234 (LT 50 = 18.44 days; LC 50 = 2.18 × 10 8 conidia/ml). The invading conidial suspensions of the most virulent isolate 2049, established in the present study, successfully evaded the antioxidant-defense mechanism, and resulted in low relative enzymatic activities of the catalases, superoxide dismutases, and glutathione S-transferases, at the lateral stages of infection. The obtained results concluded that M. anisopliae isolate 2049 could be an attractive candidate for the mycopathogen development of controlling date-palm dust mite, O. afrasiaticus.

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Section: Pathogen Virulencesupporting

confidence: 93%

“…The protease (Pr1) activity of the conidial suspensions of all tested isolates was assayed by recording the release of nitroanilide (NA) at 405 nm, using succinyl-Ala-Ala-Pro-Phe-p-nitroanilide substrate as described by Hussain et al (2015).…”

Section: Cuticle Degrading Protease Activitymentioning

confidence: 99%

Host-pathogen interaction for screening potential of Metarhizium anisopliae isolates against the date-palm dust mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae)

Hussain

Rizwan-ul-Haq

AlJabr

et al. 2019

Egypt J Biol Pest Control

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“…These three populations were separately maintained on pineapples in perforated cages (57.5 × 29 × 58 cm) at 30±1°C. Second-instar grubs were carefully shifted to an artificial diet (consisting of 45 g/L wheat flour, 45 g/L corn flour, 45 g/L yeast, 1.6 g/L sorbic acid, 4 g/L ascorbic acid, two capsules of pharmaton per litre, 500 mg/L tetracycline, and 17.5 g/L agar in 1000 mL of distilled H2O) in perforated, transparent plastic cups with a capacity of 400 mL (Hussain et al, 2015). Larvae from each regional population were reared separately at 30 ± 1°C with 75% ± 5% relative humidity in an incubator (Sanyo).…”

Section: Maintenance Of Red Palm Weevilsmentioning

confidence: 99%

“…The whole study was repeated over time. The insect, diet and frass weights were used to determine the feeding performance by calculating the efficacy of conversion of ingested food [ECI = 100 × dry weight gained by the larva/dry weight of food consumed] and the efficacy of conversion of digested food [ECD = weight gained by the larva/(food ingested -dry weight of frass)] (Hussain et al, 2009;Hussain et al, 2015). The data on the growth indices were analysed by one-way ANOVA.…”

Section: Impact Of Insecticides On the Growth And Development Of R Fmentioning

confidence: 99%

Status of Insecticide Resistance in Field-collected Populations of Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Al-Ayedh¹,

Hussain²,

Rizwan-ul-Haq³

et al. 2015

IJAB

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To cite this paper: Al-Ayedh, H., A. Hussain, M. Rizwan-ul-Haq and A.M. Al-Jabr, 2016. Status of insecticide resistance in field-collected populations of Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae AbstractThe first monitoring of the resistance to two organophosphates (methidathion and ethion) and one pyrethroid (cypermethrin) insecticides presented by three field collections of different Rhynchophorus ferrugineus (Olivier) populations was performed in this study. The LD50 doses of three insecticides against newly moulted eighth-instar R. ferrugineus larvae were estimated through toxicity bioassays, in which the larvae were fed on artificial diet supplemented with different individual doses of cypermethrin, methidathion or ethion. A population of R. ferrugineus collected from Wadi Ad-Dawasir was chosen as the reference strain, because of its susceptibility to each of the tested insecticide. Compared with the susceptible strain, populations sampled from Al-Ahsa and Al-Qatif showed 8.72 and 4.51-folds increase in resistance to cypermethrin and 3.77 and 2.85-fold increases in resistance to ethion, respectively. The LD50 values of cypermethrin (1.62 ppm), methidathion (3.15 ppm) and ethion (9.12 ppm) in the susceptible population (Wadi Ad-Dawasir) were used to investigate the feeding performances and physiological impacts on eighth-instar R. ferrugineus larvae. The larvae showed no resistance to the most potent insecticide (methidathion) resulting highest growth reduction. However, moderate resistance was observed from red palm weevil population collected from Al-Qatif and Al-Ahsa against cypermethrin resulting lowest reduction in the efficacy of conversion of ingested food (ECI) and digested food (ECD). In addition, glutathione-S-transferase (GST) activity assay further strengthen our findings. Results suggest that enhanced GST activity likely contributes to cypermethrin and ethion resistance exhibited by the R. ferrugineus populations sampled from Al-Qatif and Al-Ahsa. Toxicity bioassays, dietary utilization experiments and GST assays examined in this study support the use of methidathion against R. ferrugineus as the most potent insecticide against all the sampled populations. However, cypermethrin and ethion resistance appears to be evolving in the R. ferrugineus populations from Al-Qatif and Al-Ahsa, justifying the concern of farmers regarding the low efficacy of insecticides.

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“…Fungal entomopathogens which are natural suppressors of insects are often chosen as microbial control agents of polyphagous insect pests [13] and are considered valuable control agents of Coleoptera [11,13,14,15,16,17,18,19]. Four entomopathogenic fungi, Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae), Beauveria bassiana (Balsamo) Vuillemin (Hypocreales: Clavicipitaceae), Purpureocillium lilacinum (Thom) Luangsa-Ard, Houbraken, Hywel-Jones and Samson [20] (= Paecilomyces lilacinus ) (Hypocreales: Ophiocordycipitaceae) and Aspergillus ochraceous Wilhelm (Eurotiales: Trichocomaceae) were isolated from DRW larvae in a survey conducted monthly from June 1979 through December 1980 in nine citrus groves and one ornamental nursery in central Florida [15].…”

Section: Introductionmentioning

confidence: 99%

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

Avery

Hunter

Hall

et al. 2016

Insects

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The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 107 blastospores mL−1 on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls. Leaf residue assays were assessed by feeding the adults detached citrus leaves previously sprayed with Ifr (107 blastospores mL−1) in Petri dish chambers and then incubating them at 25 °C for 2–3 weeks. Efficacy of the soil drench applications was assessed on five larvae feeding on the roots of a Carrizo hybrid citrus seedling ~8.5–10.5 cm below the sterile sand surface in a single 16 cm × 15.5 cm pot inside a second pot lined with plastic mesh to prevent escapees. Drench treatments per pot consisted of 100 mL of Ifr suspension (107 blastospores mL−1), flushed with 400, 900, or 1400 mL of water compared to 500, 1000, and 1500 mL of water only for controls. The mean concentration of Ifr propagules as colony forming units per gram (CFUs g−1) that leached to different depths in the sand profile per treatment drench rate was also determined. Two weeks post-drenching of Ifr treatments, larvae were assessed for percent mortality, size differences, and effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. Topical spray applications caused 13 and 19% mortality in larvae and adults after 7 days compared to none in the control after 14 days, respectively. Adults feeding on a single Ifr treated leaf for 24 h consumed less than the control, and resulted in 100% mortality 35 days post-treatment compared to 33% in the untreated control. Although offered fresh, untreated leaves after 24 h, only adults in the control group consumed them. Ifr CFUs g−1 were isolated 8.5–10.5 cm below the sand surface for the 1000 and 1500 mL drench rates only, resulting in 2%–4% larval mortality. For all the Ifr drench treatments, no differences were observed in percent larval mortality and size or the effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. These results suggest that the foliar application of Ifr may be an efficient biocontrol strategy for managing adult populations of D. abbreviatus; potential alternative larval management strategies are discussed.

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Effect of Beauveria bassiana infection on the feeding performance and antioxidant defence of red palm weevil, Rhynchophorus ferrugineus

Cited by 42 publications

References 33 publications

Host-pathogen interaction for screening potential of Metarhizium anisopliae isolates against the date-palm dust mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae)

Host-pathogen interaction for screening potential of Metarhizium anisopliae isolates against the date-palm dust mite, Oligonychus afrasiaticus (McGregor) (Acari: Tetranychidae)

Status of Insecticide Resistance in Field-collected Populations of Rhynchophorus ferrugineus (Olivier) (Coleoptera: Curculionidae)

Efficacy of Topical Application, Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

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