Effect of benzothiadiazole treatment on improving the mitochondrial energy metabolism involved in induced resistance of apple fruit during postharvest storage

Li, Shenge; Jiang, Hong; Wang, Yi; Lyu, Liang; Prusky, Dov; Ji, Yan; Zheng, Xiaolin; Bi, Yang

doi:10.1016/j.foodchem.2019.125288

Cited by 62 publications

(22 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differentially expressed mitochondrial proteins have also been identified at different stages of fruit ripening 29 or in the ripening-deficient mutant 4 . In addition, mitochondrial energy metabolism and reactive oxygen species (ROS) accumulation were found to participate in the fruit defense response under postharvest pathogen infection [30][31][32] . Therefore, characterization of the mitochondrial proteome can provide important insight into the protein expression profile in response to biotic and abiotic stresses as well as facilitate a better understanding of the molecular mechanisms underlying fruit quality maintenance and ripening processes.…”

Section: Introductionmentioning

confidence: 99%

Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit

Chai

Yang

et al. 2021

Hortic Res

View full text Add to dashboard Cite

Mitochondria are crucial for the production of primary and secondary metabolites, which largely determine the quality of fruit. However, a method for isolating high-quality mitochondria is currently not available in citrus fruit, preventing high-throughput characterization of mitochondrial functions. Here, based on differential and discontinuous Percoll density gradient centrifugation, we devised a universal protocol for isolating mitochondria from the pulp of four major citrus species, including satsuma mandarin, ponkan mandarin, sweet orange, and pummelo. Western blot analysis and microscopy confirmed the high purity and intactness of the isolated mitochondria. By using this protocol coupled with a label-free proteomic approach, a total of 3353 nonredundant proteins were identified. Comparison of the four mitochondrial proteomes revealed that the proteins commonly detected in all proteomes participate in several typical metabolic pathways (such as tricarboxylic acid cycle, pyruvate metabolism, and oxidative phosphorylation) and pathways closely related to fruit quality (such as γ-aminobutyric acid (GABA) shunt, ascorbate metabolism, and biosynthesis of secondary metabolites). In addition, differentially abundant proteins (DAPs) between different types of species were also identified; these were found to be mainly involved in fatty acid and amino acid metabolism and were further confirmed to be localized to the mitochondria by subcellular localization analysis. In summary, the proposed protocol for the isolation of highly pure mitochondria from different citrus fruits may be used to obtain high-coverage mitochondrial proteomes, which can help to establish the association between mitochondrial metabolism and fruit storability or quality characteristics of different species and lay the foundation for discovering novel functions of mitochondria in plants.

show abstract

Section: Introductionmentioning

confidence: 99%

Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit

Chai

Yang

et al. 2021

Hortic Res

View full text Add to dashboard Cite

show abstract

“…Numerous scientific reports show that the resistance of fruit to adverse changes in quality during storage is related to their mitochondria metabolism (Zhou et al, 2014). Adenosine triphosphate (ATP) produced as a result of aerobic respiration in mitochondria is necessary for cells to maintain their proper activity, the integrity of membranes, and the synthesis of substances with antibacterial and antioxidant properties (Li et al, 2020;Piechowiak et al, 2021). Scientific reports also show that the use of various elicitors, i.e., oxalic acid, benzothiazole, methyl jasmonate, or 1-methylcyclopropene, increases the activity of mitochondria, which corresponds to better fruit quality during storage (Jin et al, 2013(Jin et al, , 2014Li et al, 2020;Shu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Adenosine triphosphate (ATP) produced as a result of aerobic respiration in mitochondria is necessary for cells to maintain their proper activity, the integrity of membranes, and the synthesis of substances with antibacterial and antioxidant properties (Li et al, 2020;Piechowiak et al, 2021). Scientific reports also show that the use of various elicitors, i.e., oxalic acid, benzothiazole, methyl jasmonate, or 1-methylcyclopropene, increases the activity of mitochondria, which corresponds to better fruit quality during storage (Jin et al, 2013(Jin et al, , 2014Li et al, 2020;Shu et al, 2020). In view of the above, it should be assumed that a reduction of adverse changes in the quality of fruit during storage under the influence of ozone may result from both the strong antimicrobial properties of ozone and the influence of ozone on the energy metabolism of the fruit.…”

Section: Introductionmentioning

confidence: 99%

The Role of Mitochondrial Energy Metabolism in Shaping the Quality of Highbush Blueberry Fruit During Storage in Ozone-Enriched Atmosphere

Piechowiak

Sowa

Tarapatskyy

et al. 2021

Food Bioprocess Technol

View full text Add to dashboard Cite

The aim of this study was to evaluate the effect of ozone treatment on the mitochondrial energy metabolism in blueberry fruit during storage as well as to determine the relationship between the activity of mitochondria and the antioxidative properties of ozonated fruit. Blueberry fruit was stored for 28 days at 4 °C and ozonated daily with gaseous ozone at the concentration of 15 mg L−1 for 30 min, every 12 h of storage. Research showed that ozonated fruit was characterized by higher activity of enzymes involved in oxidative phosphorylation (by 58.7% for SDH, 118.2% for CCO, and 78.16% for H+-ATPase after 7 days, respectively) than non-ozonated sample, which contributed to reduction of the loss of energy charge and ATP in the fruit during storage. Moreover, the increased activity of mitochondria led to the growth of mitochondrial ROS accumulation which, in turn, activated defense mechanisms against oxidative stress in the fruit. These metabolic responses might collectively contribute to increase the antioxidative properties of ozonated fruit and consequently to maintain a good quality of the fruit over a long period of storage.

show abstract

“…Recently, BTH treatment has been considered as a new technology to substitute for the fungicides to enhance disease resistance and reduce fruit decay in different fruits, such as strawberry, grapes, sweet cherries, peaches, and apples. [6][7][8][9][10] Moreover, other studies in the literature have reported that BTH treatment induced soluble sugar accumulation in grape berries, 11 increased anthocyanin biosynthesis in strawberry, 12 enhanced respiration rate and ethylene production in muskmelon, 13 and maintained fruit firmness, color and good fruit quality in banana, 14 which indicated BTH treatment was a promising method for regulating ripening progress and quality in some selected fruit and vegetables during postharvest. However, no information was available regarding the influence of BTH treatment on plum fruit.…”

Section: Introductionmentioning

confidence: 92%

Effect of benzothiadiazole treatment on quality and anthocyanin biosynthesis in plum fruit during storage at ambient temperature

Chen

Shuling

et al. 2020

J Sci Food Agric

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Plums tend to experience a reduction in fruit quality due to ripening and they deteriorate quickly during storage at room temperature. Benzothiadiazole (BTH) is a plant elicitor capable of inducing disease resistance in many crops. In this study, the effect of BTH treatment on fruit ripening, fruit quality, and anthocyanin biosynthesis in 'Taoxingli' plum was investigated. RESULTS:The results showed that BTH treatment could accelerate fruit ripening without affecting the incidence of fruit decay or the shelf life. Benzothiadiazole treatment improved the quality and consumer acceptability of 'Taoxingli' plums during storage by increasing the sweetness, red color formation, and the concentration of healthy antioxidant compounds. The BTH treatment could also effectively promote the biosynthesis of anthocyanin by enhancing the enzyme activities of phenylalanine ammonia-lyase (PAL), dihydroflavonol 4-reductase (DFR), anthocyanidin synthase (ANS), and uridine diphosphate flavonoid 3-O-glucosyltransferase (UFGT) and up-regulating the gene expressions of PsPAL, PsCHI, PsDFR, PsANS, and PsUFGT during storage.CONCLUSION: Benzothiadiazole treatment could be a potential postharvest technology for improving fruit quality and consumer acceptability in harvested plum fruit.

show abstract

Effect of benzothiadiazole treatment on improving the mitochondrial energy metabolism involved in induced resistance of apple fruit during postharvest storage

Cited by 62 publications

References 33 publications

Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit

Isolation and comparative proteomic analysis of mitochondria from the pulp of ripening citrus fruit

The Role of Mitochondrial Energy Metabolism in Shaping the Quality of Highbush Blueberry Fruit During Storage in Ozone-Enriched Atmosphere

Effect of benzothiadiazole treatment on quality and anthocyanin biosynthesis in plum fruit during storage at ambient temperature

Contact Info

Product

Resources

About