Effect of Beta-2-Thienylalanine on Developing Mouse Tooth Germs In Vitro

Abstract

Tooth germs grown on Eagle's medium solidified with agar will differentiate in vitro. Normal development was suppressed by beta-2-thienylalanine (1 or 2 millimols per liter), but it proceeded when phenylalanine and beta-2-thienylalanine were present in equimolar concentrations. This effect may have been due to interference with protein metabolism in the dermal papillar cells.

Neural Crest Cells in Early Tooth Germ of Mice Embryos

“…These results have been gained by experimental embryology in urodela ; vital staining, ectopic grafting and explantation. On the other hand, though a concrete indication was lacking in the higher vertebrate, it has been discussed that the tooth germ is related with the neural crest (Pourtois, 1961(Pourtois, , 1966Miles and Gaunt, 1967 ;Slavkin and Bavetta, 1968 ;Beierle and Bavetta, 1968 ;Moss, 1969 ;Kollar and Baird, 1968Tonge, 1971).…”

“…Though a considerable volume of literature on the neural crest cells related to the morphogenesis of the tooth has been reported (Plat, 1893(Plat, , 1894(Plat, , 1896(Plat, , 1897Landacre, 1921 ;Adams, 1924Adams, , 1931Raven 1932Raven , 1935StrOr, 1933 ;Holtfreter, 1933Holtfreter, , 1935aKawakami, 1941 ;Sellman, 1946 ;De Beer, 1947 ;Pourtois, 1961Pourtois, , 1966Chibon, 1967 ;Gaunt and Miles, 1967 ;Moss, 1969 ;Kollar and Baird, 1968Tonge, 1971), concrete indication about the neural crest cells in tooth germ of the mammales is scanty.…”

Effects of mitomycin C in postnatal tooth development in mice with special reference to neural crest cells

“…, 1935Slriir, 1933;Hollfreler, 1933Hollfreler, , 1935aSell man, 1946;De Beer. 1947;Pounds, 1961Pounds, , 1964Cliihon, 1967;Kollar and Baird, 1968. 1969, 1971Moss.…”

Deficiency of Enamel Knot in Experimental Morphology

“…morphogenesis (Pourtois, 1961(Pourtois, , 1964Slavkin, Beierle and Bavetta, 1968 ;Miller, 1969Miller, , 1971Moss, 1969 ;Kollar and Baird, 1968Tonge, 1971). However, little is known about the relation between the enamel knot and mesenchyme with reference to the morphogenesis.…”