Effect of Beta&amp;ndash;Sitosterol on Transforming Growth Factor&amp;ndash;Beta&amp;ndash;1 Expression and Translocation Protein Kinase C Alpha in Human Prostate Stromal Cells in vitro

Kassen, Annette; Berges, Richard; Senge, Theodor

doi:10.1159/000020227

Cited by 22 publications

(8 citation statements)

References 32 publications

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“…We found that B-Sitosterol inhibited the growth of LNCaP cells. This agrees with Von Holtz et al [31] who reported that BSitosterol inhibited the growth of LNCaP cells and induced apoptosis via activation of the sphingomyelin cycle or by regulation of the activity of PKC-alpha [34]. Also Awad et al [27] reported that B-Sitosterol might affect membrane structure and function of tumor and host tissues, signal transduction pathways that regulate tumor growth, and apoptosis However, we found no effect of B-Sitosterol on PC-3 cell growth but we did see an effect similar to Awad et al [32] who showed that B-sitosterol inhibited the growth of LNCaP cells in vitro.…”

Section: Discussionsupporting

confidence: 92%

Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

Shenouda

Sakla

Newton

et al. 2007

Endocr

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Section: Discussionsupporting

confidence: 92%

Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

Shenouda

Sakla

Newton

et al. 2007

Endocr

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“…A plant-derived sterol mixture including BSS was found to inhibit the recruitment of neutrophils in a murine model of carrageenan-induced inflammation in vivo (Navarro et al, 2001). The effects of BSS on pro-inflammatory signal transduction have not been extensively investigated, the only hint being that BSS was found to inhibit the active form of the PKCα in prostate-derived cells (Kassen et al, 2000). Here we found that BSS partly reduces the activation of the PKCα isoform in a completely different model, namely the bronchial epithelial cells challenged with P. aeruginosa ( Figure 7 ), which we found involved with a relevant pro-inflammatory role in the Ca 2+ -dependent signaling machinery, leading to IL-8 gene expression induced by P. aeruginosa in CF bronchial epithelial cells (Bezzerri et al, 2011b).…”

Section: Discussionmentioning

confidence: 99%

β-Sitosterol Reduces the Expression of Chemotactic Cytokine Genes in Cystic Fibrosis Bronchial Epithelial Cells

et al. 2017

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Extracts from Nigella arvensis L. seeds, which are widely used as anti-inflammatory remedies in traditional medicine of Northern Africa, were able to inhibit the expression of the pro-inflammatory neutrophil chemokine Interleukin (IL)-8 in Cystic Fibrosis (CF) bronchial epithelial IB3-1 cells exposed to the Gram-negative bacterium Pseudomonas aeruginosa. The chemical composition of the extracts led to the identification of three major components, β-sitosterol, stigmasterol, and campesterol, which are the most abundant phytosterols, cholesterol-like molecules, usually found in plants. β-sitosterol (BSS) was the only compound that significantly reproduced the inhibition of the P. aeruginosa-dependent expression of IL-8 at nanomolar concentrations. BSS was tested in CF airway epithelial CuFi-1 cells infected with P. aeruginosa. BSS (100 nM), showed a significant and consistent inhibitory activity on expression of the P. aeruginosa-stimulated expression chemokines IL-8, GRO-α GRO-β, which play a pivotal role in the recruitment of neutrophils in CF inflamed lungs. Preliminary mechanistic analysis showed that BSS partially inhibits the P. aeruginosa-dependent activation of Protein Kinase C isoform alpha, which is known to be involved in the transmembrane signaling activating IL-8 gene expression in bronchial epithelial cells. These data indicate BSS as a promising molecule to control excessive lung inflammation in CF patients.

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“…The plant sterols can inhibit the cholesterol absorption in rats (Ikeda I, 1988), and has the abilities of cholesterol lowering (David K, 2005), tumor inhibition (Awad A B, 2001), prevention the hypertrophy of prostate (Kassen A, 2000). One such plant sterols which has gained wide attention of medical professionals, pharmaceutical marketers, and researchers all around the world is Stigmasterol.…”

Section: Discussionmentioning

confidence: 99%

Effects of Stigmasterol to Activities of Several Enzymes of Tetranychus cinnabarinus

Duan

Cheng

et al. 2012

Advances in Intelligent and Soft Computing

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Abstract. The acaricidal activity of stigmasterol was carried out on the Tetranychus cinnabarinus, using the slide dip technique. Here the female adult mites were treated by the Stigmasterol extracted from Inula japonica. Bioassayguided purification of the 12th fraction had higher activity against the T. cinnabarinus, Gas chromatography-mass spectrometry (GC/MS) demonstrated that the chemical composition of the active fraction 12th sample was mainly composed of Stigmasterol. That had not been reported before. And the acaricidal mechanism of Stigmasterol to T. cinnabarinus is unknown. Our results showed that the nervous symptoms of the T. cinnabarinus in response to the Stigmasterol. The protein content, the acetylcholine esterase (AChE) activity, the Calcium enzyme ATP (Ca2+-ATPase) activity and Sodium enzyme ATP (Na+-ATPase) activity of the mites treated with Stigmasterol were assayed. The results showed that the protein content and the activity of AchE in the T. cinnabarinus treated with the Stigmasterol were increased. The activity of Ca2+-ATPase and Na+-ATPase were induced by the Stigmasterol. The effects of the T. cinnabarinus indicated that the central nervous system was the main target of Stigmasterol. The long time over excitation caused the treated mites to die.

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Effect of Beta–Sitosterol on Transforming Growth Factor–Beta–1 Expression and Translocation Protein Kinase C Alpha in Human Prostate Stromal Cells in vitro

Cited by 22 publications

References 32 publications

Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

Phytosterol Pygeum africanum regulates prostate cancer in vitro and in vivo

β-Sitosterol Reduces the Expression of Chemotactic Cytokine Genes in Cystic Fibrosis Bronchial Epithelial Cells

Effects of Stigmasterol to Activities of Several Enzymes of Tetranychus cinnabarinus

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