Effect of betamethasone treatment on luteal lifespan and the LH response to GnRH in dairy cows

Dobson, H.; Alam, M. G. S.; Kanchev, L.N.

doi:10.1530/jrf.0.0800025

Cited by 24 publications

(24 citation statements)

References 9 publications

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“…This conclusion is further supported by the known ability of glucocorticoids to block eicosanoid synthesis. Glucocorticoids inhibit uterine PG production in the rat [32,33] and the cow [34], and such treatment extended luteal function in the latter species [34]. In the present studies, dexamethasone was shown to severely impair PGE 2 and PGF 2 a production in ovarian cell dispersate.…”

Section: Hods Each Well Contained 15 Million Cells Incubatedsupporting

confidence: 52%

Immunosuppressive Levels of Glucocorticoid Block Extrauterine Luteolysins in the Rat1

Wang¹,

Riley²,

Behrman³

1993

Biology of Reproduction

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Leukocytes, normal constituents of the corpus luteum, release prostaglandins (PGs), superoxide, and hydrogen peroxide (H2O2) upon activation. These products appear to mediate luteolysis, and it has been postulated that activated leukocytes serve a role in corpus luteum regression. Glucocorticoids prevent leukocyte infiltration and activation by inhibiting cytokine production, secretion, and action, and also inhibit eicosanoid synthesis. The objective of the present studies was therefore to assess whether glucocorticoid would influence luteal regression in the rat. Ovulation and pseudopregnancy were synchronized in prepubertal rats by gonadotropin treatment. In uterine-intact rats, functional luteal regression, assessed from serum progesterone levels, began on Day 10 and was complete by Day 14. Dexamethasone blocked luteal regression in uterine-intact animals when administered daily from early in pseudopregnancy for as long as treatment was continued (up to Day 17). Immunosuppressive effects of dexamethasone were evident in the inhibition of estrogen-induced infiltration of eosinophils, as shown by abrogation of estrogen-induced uterine peroxidase activity, and high continuous levels of dexamethasone were necessary to block luteolysis. Hysterectomy extended luteal function by several days, with maintenance of maximal serum progesterone levels up to Day 12; but serum progesterone levels were reduced about 50% by Day 16 and completely depressed by Day 19. Dexamethasone treatment of hysterectomized rats from Day 12 until Day 15 or 18 blocked the decline in serum progesterone levels. Dexamethasone treatment did not block the decrease in serum progesterone levels induced within 24 h by PGF2 alpha in uterine-intact animals on Day 9 or in hysterectomized animals on Day 19.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Hods Each Well Contained 15 Million Cells Incubatedsupporting

confidence: 52%

Immunosuppressive Levels of Glucocorticoid Block Extrauterine Luteolysins in the Rat1

Wang¹,

Riley²,

Behrman³

1993

Biology of Reproduction

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“…On the other hand, indomethacin given later in the oestrous cycle resulted in an extended CL life span (Milvae 1986). Repeated administrations of a synthetic glucocorticoid, betamethasone, during the luteal phase have also been shown to prolong luteal life span in cattle (Kanchev et al 1976, Dobson et al 1987. Although these effects are likely to be due to the inhibitory action of these agents on prostaglandin F 2 synthesis by the uterine endometrium or/and on pituitary function (Dobson et al 1987), it is possible that glucocorticoid also acts directly to modulate CL function.…”

Section: Discussionmentioning

confidence: 99%

“…Repeated administrations of a synthetic glucocorticoid, betamethasone, during the luteal phase have also been shown to prolong luteal life span in cattle (Kanchev et al 1976, Dobson et al 1987. Although these effects are likely to be due to the inhibitory action of these agents on prostaglandin F 2 synthesis by the uterine endometrium or/and on pituitary function (Dobson et al 1987), it is possible that glucocorticoid also acts directly to modulate CL function. Glucocorticoid inhibits prostaglandin synthesis in rat ovary (Kol et al 1998), and inactivation of glucocorticoid by 11HSD2 appears to be necessary for the regression of CL in the pregnant rat just prior to parturition (Waddell et al 1996).…”

Section: Discussionmentioning

confidence: 99%

Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum

Tetsuka¹,

Yamamoto²,

Hayashida³

et al. 2003

Journal of Endocrinology

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In glucocorticoid target organs, local concentrations of active glucocorticoid are determined by the relative expression of two 11 -hydroxysteroid dehydrogenases (HSDs): bi-directional 11 -HSD type1 (11HSD1) that mainly activates cortisone to cortisol, and dehydrogenase 11 -HSD type2 (11HSD2) that inactivates cortisol to cortisone. In this study, we examined the expression of mRNA encoding these two 11 -HSDs in bovine granulosa cells harvested from preovulatory follicles and corpora lutea (CL). Ovaries were obtained from Holstein cows at a local slaughterhouse. Follicles larger than 10 mm in diameter and CL were dissected and follicular fluid and granulosa cells were taken. Corpora lutea were weighed and their stages were morphologically assessed (stage I, days 1-4; stage II, days 5-10; stage III, days 11-17; stage IV, days 8-20). Follicles were classified into four groups according to their hormonal status (oestradiol (E 2 ): progesterone (P 4 )>1: oestrogen active; E 2 :P 4 <1: oestrogen inactive) and stage of the oestrous cycle (luteal or follicular phase). Total RNA was extracted with phenol-chloroform and subjected to a semi-quantitative RT-PCR for 11HSD1, 11HSD2 and -actin. Concentrations of steroids in follicular fluid were determined by an enzyme immunoassay. In granulosa cells, only 11HSD1 mRNA was detected. There was a negative correlation between the expression of 11HSD1 and the concentration of cortisol in follicular fluid (P<0·05), indicating 11HSD1 may act as a dehydrogenase in the bovine follicle. Both types of 11 -HSDs were expressed in CL. The levels of mRNA for both isozymes were high in stage I and II, and were decreased in stage III CL. In stage IV CL, the expression of 11HSD2 but not 11HSD1 mRNA increased. These results indicate that the bovine granulosa cells and CL express 11HSD1 and 11HSD2, and they may play an important physiological role in the bovine ovary through modulating the local glucocorticoid environment.

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“…In sheep, stress has been shown to induce GC secretion and suppression of reproductive neuroendocrine function and ovarian cyclicity [10,11]. Moreover, repeated administrations of a synthetic GC (betamethasone) during the luteal phase prolong luteal life span in cattle [12,13]. Although it is possible that GC directly regulates corpus luteum (CL) function, the roles of GC in the CL remain unknown.…”

Section: Introductionmentioning

confidence: 99%

Cortisol Is a Suppressor of Apoptosis in Bovine Corpus Luteum1

Komiyama

Nishimura

Lee

et al. 2008

Biology of Reproduction

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Glucocorticoid (GC) acts as a modulator of physiological functions in several organs. In the present study, we examined whether GC suppresses luteolysis in bovine corpus luteum (CL). Cortisol (an active GC) reduced the mRNA expression of caspase 8 (CASP8) and caspase 3 (CASP3) and reduced the enzymatic activity of CASP3 and cell death induced by tumor necrosis factor (TNF) and interferon gamma (IFNG) in cultured bovine luteal cells. mRNAs and proteins of GC receptor (NR3C1), 11beta-hydroxysteroid dehydrogenase type 1 (HSD11B1), and HSD11B2 were expressed in CL throughout the estrous cycle. Moreover, the protein expression and the enzymatic activity of HSD11B1 were high at the early and the midluteal stages compared to the regressed luteal stage. These results suggest that cortisol suppresses TNF-IFNG-induced apoptosis in vitro by reducing apoptosis signals via CASP8 and CASP3 in bovine CL and that the local increase in cortisol production resulting from increased HSD11B1 at the early and midluteal stages helps to maintain CL function by suppressing apoptosis of luteal cells.

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Effect of betamethasone treatment on luteal lifespan and the LH response to GnRH in dairy cows

Abstract: Summary. Betamethasone

Cited by 24 publications

References 9 publications

Immunosuppressive Levels of Glucocorticoid Block Extrauterine Luteolysins in the Rat1

Immunosuppressive Levels of Glucocorticoid Block Extrauterine Luteolysins in the Rat1

Expression of 11beta-hydroxysteroid dehydrogenases in bovine follicle and corpus luteum

Cortisol Is a Suppressor of Apoptosis in Bovine Corpus Luteum1

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