Effect of Betulin-containing Extract from Birch Tree Bark on α-Amylase Activity In vitro and on Weight Gain of Broiler Chickens In vivo

Abstract

In vitro effect of betulin-containing extract from Betula pendula Roth. bark on alpha-amylase activity was studied, the kinetic mechanism of interaction was proposed and in vivo effect of betulin-containing extract on weight gain and meat quality of broiler chickens was evaluated. The highest level of inhibitory activity (20%) was detected in extract concentration of 1,000 mg/L. Increased extract concentration did not lead to increased enzyme inhibition. Using Dixon and Cornish-Bowden coordinates, the competitive mechanism of inhibition was demonstrated. Calculated kinetic parameters were: Km equal to 0.6 mg/mL, Vmax equal to 2.6 and 2.1 mM/min from Lineweaver-Burk and Dixon coordinates, respectively and Ki equal to 3,670 ± 230 mg/mL. The partial inhibition of enzyme indicates the existence of low concentration of active inhibitory form, which reaches saturation level with increased extract concentration in applied suspension. Therefore, Ki has an apparent constant character. This partial inhibition of amylase activity observed in in vitro assay did not affect weight gain and meat quality of broiler chickens during in vivo assay. Rather, the tendency to increase the weight of edible parts and muscles compared to diet without additive suggests that the extract may be a potential food additive in poultry farming. Additionally, it could be a source for further pharmaceutical and pharmacological research.

Altering the inhibitory kinetics and molecular conformation of maltase by Tangzhiqing (TZQ), a natural α-glucosidase inhibitor

“…For anticompetitive inhibition, the lines are parallel. When a mixed inhibition mechanism existed, the data lines intersect in the second or third quadrant on the Line-weaver Burk plots [ 24 – 26 ]. The results showed that the substrate concentration lines intersected at the y-axis, and with the increase in the concentration of TZQ, no change was observed in the V max values, while the K m values increased, indicating that this sample acted as a competitive inhibitor, similar to acarbose [ 27 – 29 ].…”

Synthesis and Fabrication of Betulin-Derived Polysulfide and Polysulfoxide Electrospun Fibers for Fruit Preservation

“…Terpene-containing acrylate monomers have also been synthesized and polymerized via free-radical polymerizations in toluene and by bulk, achieving significant conversion and high molecular weights (up to 278 kg/mol) . Betulin, a pentacyclic triterpene obtained from the bark of white birch, displays potent antibacterial properties and has been investigated for its therapeutic potential in treating skin issues such as eczema and psoriasis. , Previous studies have transformed betulin into methacrylate monomers, which were used to construct amphiphilic copolymers through controlled/living radical polymerization. , By modifying betulin into olefin derivatives with varying methylene spacer lengths between the olefin and the ester group, the synthesis of cycloolefins through ring-closing metathesis and polyolefins through acyclic diene metathesis polymerization was achieved. , These betulin-containing materials hold significant importance in renewable polymers, with their triterpenoid derivatives offering potential for exploration in biological activities. − To the best of our knowledge, the combination of thiol–ene polymerization with betulin derivatives for the design and synthesis of new polymers to explore their bioapplications and biological characteristics has not been reported previously.…”

Structure Identification of Triterpene from the Mushroom Pleurotus eryngii with Inhibitory Effects Against Breast Cancer

“…Increasing evidence of their wide spectrum of pharmacological effects coupled with low-toxicity profiles have sparked renewed interest regarding their effects on human health and disease. Triterpenoids are widely used in Asian traditional medicines due to their multifunctional activities, such as antibacterial, anti-inflammatory, hypolipidemic, hepatoprotective, cardiovascular, and analgesic effects [11][12][13][14]. Emerging studies indicate that triterpenoids exert anti-tumour activities in various in vitro and in vivo model systems, without manifesting toxicity in normal cells [15][16][17].…”