Effect of Bile-Duct Ligation on Organelle Marker Enzymes in the Liver and Serum of Rats

Abstract

1. Marker enzymes for the principal subcellular organelles of rat liver were asayed in the liver of rats 1 day and 8 days after bile-duct ligation or after laparotomy as a control procedure. 2. The microsomal enzymes in liver tissue showed complex changes. Benz[alpha]pyrene hydroxylase activity, predominantly found in the smooth endoplasmic reticulum, was decreased. Glucose 6-phosphatase activity and ribonucleic acid, which are localized predominantly in the rough endoplasmic reticulum, were increased. 3. The plasma membrane enzyme, alkaline phosphatase, increased in activity after bile-duct ligation. 4. No changes in mitochondrial enzyme activities were noted after 1 day but there was a 50% reduction 8 days after ligation. Lysosomal enzyme activities did not change in the liver tissue. 5. Liver catalase and D-amino acid oxidase activities showed a slight increase at 1 day postligation but a significant fall by 8 days. 6. Lactate dehydrogenase, a cytosol enzyme, showed a decrease in activity after 1 day but an increase in tissue activities 8 days after ligation. 7. Serum activities of mitochondrial, plasma membrane, microsomal, lysosomal and cytosol marker enzymes tended to increase post-ligation, particularly at 8 days. 8. Monoamine oxidase, a predominantly mitochondrial enzyme, was greatly elevated in the serum after 1 day but had returned to normal activities by 8 days.

Tumor Necrosis Factor α-dependent Up-regulation of Lrh-1 and Mrp3(Abcc3) Reduces Liver Injury in Obstructive Cholestasis

“…Serum Lactate Dehydrogenase (LDH) Assay-Serum stored at Ϫ20°C was used for this assay (20,21), which was performed according to the manufacturer's protocol (Roche Applied Science). Briefly, 100 l of serum was transferred into an optically clear 96-well flat-bottomed microtiter plate.…”

Enzyme Activities in Human Liver Biopsies: Assay Methods and Activities of Some Lysosomal and Membrane-Bound Enzymes in Control Tissue and Serum

“…Under identical assay conditions the specific activities of acid hydrolases in rat liver were between two and ten times those of human liver (Peters, Neale & Heath, 1975), but their relative activities were similar. Acid phosphatase was the most active, followed by 8-glucuronidase and N-acetyl-8-glucosaminidase.…”