Effect of bile on the lipid composition and surface properties of bifidobacteria

Gómez-Zavaglia, Andrea; Kociubinski, Guillermo; Pérez, Pablo; Disalvo, E.A.; Antoni, Graciela L. De

doi:10.1046/j.1365-2672.2002.01747.x

Cited by 105 publications

(68 citation statements)

References 12 publications

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“…Components mediating the adherence inhibition are increased in concentration or become more efficiently exposed as adhesins or receptors when the yeast cells are disrupted or hydrolysed. However, bile salts may reduce the adherence efficiency as shown by Guglielmetti et al and Gomez-Zavaglia et al [11,12].…”

Section: Discussionmentioning

confidence: 97%

“…Gomez-Zavaglia et al has proposed that changes in surface and adherence properties may correlate with changes in sugar components induced by bile during growth. Attribution of morphological and physical properties to binding potential of yeast cells can only be hypothesized from the present study and needs to be addressed in more detail in future [11].…”

Section: Discussionmentioning

confidence: 99%

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Morphological Characterization of Pichia guilliermondii and Saccharomyces cerevisiae Yeast and their Effects on Adherence of Intestinal Pathogens on Piglet and Chicken Epithelium In-vitro

Peisker¹,

Stensrud²,

Apajalahti³

et al. 2017

J Anim Res Nutr

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Intestinal pathogen binding effects are related to mannanoligocaccharides that occur in several micro-organism species e.g. fungi and yeasts. Binding varies between species depending on their size, structure and cell wall properties. The purpose of the study was to elucidate differences in adherence potential of commercially used yeast cells and possibly determine causative elements for the observed differences. Electron microscopy and surface hydrophobicity measurements reveal that Pichia guilliermondii yeast possesses distinguished traits compared to Saccharomyces cerevisiae that may account for observed differences in pathogen binding efficiency. Pichia guilliermondii significantly inhibited adherence of pathogenic strains of Escherichia coli and Salmonella enterica in small intestinal epithelium of swine and broiler chicken. Treatment of Pichia yeast with digestive fluids significantly increased this effect. In-vivo, gastric activation occurs naturally as verified by broiler assays with P. guilliermondii. P. guilliermondii and S. cerevisiae retained their inhibitory effects on pathogenic E. coli adherence after passing through the upper digestive tract. Jejunal digesta recovered from birds treated with P. guilliermondii showed higher inhibitory effect than digesta from birds treated with the S. cerevisiae yeast. The inhibitory effect was still detectable in ileal digesta. Intact, non-gastric treated P. guilliermondii was as effective as the gastric pre-treated yeast, suggesting that digestive enzymes of broiler chicken were capable of activating yeast cells in-situ.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Morphological Characterization of Pichia guilliermondii and Saccharomyces cerevisiae Yeast and their Effects on Adherence of Intestinal Pathogens on Piglet and Chicken Epithelium In-vitro

Peisker¹,

Stensrud²,

Apajalahti³

et al. 2017

J Anim Res Nutr

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show abstract

“…Amongst those mechanisms are the bile efflux (17,18,19), counteracting H + accumulation by ATPase F0F1 pump (20), modification of plasma membrane composition (21)(22)(23) and bile salts deconjugation through the activity of bile-salt-hydrolases (BSHs) (24)(25)(26). The bile salts hydrolases produced by lactic acid bacteria are responsible for the deconjugation of bile salts with the formation of amino acid residues and deconjugated bile acids.…”

Section: Discussionmentioning

confidence: 99%

Bile salt hydrolase and antimicrobial activities of three bile resistant probiotics Lactobacillus plantarum strains isolated from Cameroonian artisanal fermented milk

Landry¹,

Pierre-Marie²,

Hector³

et al. 2017

J Microbiol Biotech Res

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“…The structure and composition of the bacterial surface have a profound influence on bile tolerance. More concisely, lipid composition, membrane protein profiles and cell wall functionality play key roles in the response to bile in the genus Bifidobacterium [22,44,67]. Production of extracellular polysaccharides (exopolysaccharides; EPSs) is also a mechanism mediating protection against bile in this genus [3,63], and a direct correlation has recently been established between EPS production and bile tolerance in Bifidobacterium spp.…”

Section: Bile Saltsmentioning

confidence: 99%

How do bifidobacteria counteract environmental challenges? Mechanisms involved and physiological consequences

et al. 2011

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An effective response to stress is of paramount importance for probiotic bifidobacteria administered in foods, since it determines their performance as beneficial microorganisms. Firstly, bifidobacteria have to be resistant to the stress sources typical in manufacturing, including heating, exposure to low water activities, osmotic shock and presence of oxygen. Secondly, and once they are orally ingested, bifidobacteria have to overcome physiological barriers in order to arrive in the large intestine biologically active. These barriers are mainly the acid pH in the stomach and the presence of high bile salt concentrations in the small intestine. In addition, the large intestine is, in terms of microbial amounts, a densely populated environment in which there is an extreme variability in carbon source availability. For this reason, bifidobacteria harbours a wide molecular machinery allowing the degradation of a wide variety of otherwise non-digestible sugars. In this review, the molecular mechanisms allowing this bacterial group to favourably react to the presence of different stress sources are presented and discussed.

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Effect of bile on the lipid composition and surface properties of bifidobacteria

Abstract: Long-term exposure of bacteria to bile may cause metabolic changes affecting their adhesive properties irreversibly. This may be taken as a criterion to define the probiotic properties of different strains.

Cited by 105 publications

References 12 publications

Morphological Characterization of Pichia guilliermondii and Saccharomyces cerevisiae Yeast and their Effects on Adherence of Intestinal Pathogens on Piglet and Chicken Epithelium In-vitro

Morphological Characterization of Pichia guilliermondii and Saccharomyces cerevisiae Yeast and their Effects on Adherence of Intestinal Pathogens on Piglet and Chicken Epithelium In-vitro

Bile salt hydrolase and antimicrobial activities of three bile resistant probiotics Lactobacillus plantarum strains isolated from Cameroonian artisanal fermented milk

How do bifidobacteria counteract environmental challenges? Mechanisms involved and physiological consequences

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