Effect of Biochar and Green Manure on Soil Nutrients and Rhizosphere Soil Fungal Community Structure

Abstract

In order to explore the impact of biochar and green manure on the micro-ecological environment of citrus soil, a field experiment was used to set CK (conventional fertilization), T1 (5.0 kg/biochar) and T2 (5.0 kg/biochar + 10% nitrogen fertilizer reduction + green manure), for measuring the 3 kinds of fertilization affectness to the fungal diversity and community structure of citrus rhizosphere soil. The results showed that, compared with CK, the pH, alkali-hydrolyzable nitrogen, available phosphorus and available potassium content of T1 and T2 treatments were significantly increased, and soil bulk density was significantly reduced. Application of only biochar increased the diversity of soil fungi, but reduced the richness of fungi. Combined application of biochar and green manure significantly increased the diversity and richness of fungi. Application of biochar increased the relative abundance of Basidiomycota, Schizoplasmodiida, and Talaromyces in soil, and decreased relative abundance of Ascomycota, Mucoromycota, Chaetomium and Fusarium. The study shows that the combined application of biochar and green manure can significantly increase the soil fungal diversity, reconstruct community structure of soil fungi, reduce the abundance of pathogenic bacteria, and promote micro-ecological balance in soil for citrus and soil health。

Impact of Straw Incorporation on the Physicochemical Profile and Fungal Ecology of Saline–Alkaline Soil

“…The observed inverse relationship between pH levels and total nitrogen (TN) content, as well as microbial communities, aligns with previous investigations [66,67]. Consequently, the makeup of soil microbial communities is impacted [68], thereby highlighting the significance of organic matter as a key determinant of fungal communities [69]. The findings of AN and AP in a study conducted by Sun et al [70] exhibited a resemblance to the results observed in the present investigation.…”