One of the main conditions for the stable growth of grain production of spring barley is the expansion of sown areas, using varieties with high adaptive properties and capable of providing high yields in a changing climate. The purpose of the work is to determine the drought resistance and heat resistance of varieties and lines of spring barley using laboratory methods. The article presents studies of physiological indicators of resistance at an early stage of plant development to extreme environmental factors (drought and relatively high air temperatures). The physiological method of early diagnostics of seeds and seedlings provides information on the general initial level of physiological and biochemical processes in germinating seeds under stressful conditions and allows one to get an idea of the resistance of adult plants. Such a primary assessment gives grounds for the selection of promising samples for a deeper study of their stability. When determining the resistance of varieties and lines of spring barley to abiotic stressors, samples were identified that, in terms of a set of indicators (drought resistance, heat resistance, degree of depression, index of complex resistance and growth of germinal roots), values significantly exceeding the standard variety Ratnik (36.6; 91, 1; 2.13%; 184.3 rel. units and 2.78 cm, respectively): Zernogradsky 1717 (45.6; 84.0; 5.19%; 194.8 rel. units and 4.76 cm), Zernogradsky 1716 (4.3; 83.4; 5.43%; 188.0 relative units and 3.67 cm), Zernogradsky 1701 (36.2; 87.0; 3.17%; , 4 relative units and 2.56 cm).