Effect of biofilm formation, and biocorrosion on denture base fractures

Abstract

PURPOSEThe aim of this study was to investigate the destructive effects of biofilm formation and/or biocorrosive activity of 6 different oral microorganisms.MATERIALS AND METHODSThree different heat polymerized acrylic resins (Ivocap Plus, Lucitone 550, QC 20) were used to prepare three different types of samples. Type "A" samples with "V" type notch was used to measure the fracture strength, "B" type to evaluate the surfaces with scanning electron microscopy and "C" type for quantitative biofilm assay. Development and calculation of biofilm covered surfaces on denture base materials were accomplished by SEM and quantitative biofilm assay. According to normality assumptions ANOVA or Kruskal-Wallis was selected for statistical analysis (α=0.05).RESULTSSignificant differences were obtained among the adhesion potential of 6 different microorganisms and there were significant differences among their adhesion onto 3 different denture base materials. Compared to the control groups after contamination with the microorganisms, the three point bending test values of denture base materials decreased significantly (P<.05); microorganisms diffused at least 52% of the denture base surface. The highest median quantitative biofilm value within all the denture base materials was obtained with P. aeruginosa on Lucitone 550. The type of denture base material did not alter the diffusion potential of the microorganisms significantly (P>.05).CONCLUSIONAll the tested microorganisms had destructive effect over the structure and composition of the denture base materials.

A nanostructured anti-biofilm surface widens the efficacy against spindle-shaped and chain-forming rod-like bacteria

“…4 The material itself may become weakened. 8 The oral cavity is the most microbially polluted environment in the human body, such that more than 600 different species of bacteria and over 100 million bacterial cells may be recovered in every milliliter of saliva. 9 A fraction of this number is potential pathogens or existing ones.…”

Multiple Risk Factors of Candida Albicans Associated Denture Stomatitis

“…Huh et al and Hahnel et al reported that significantly higher number of Candida albicans was observed on roughened than on smooth surfaces [22,28]. Denture base cracks may become to be one of the best sites for microorganism propagation and provides protection from shear forces, even during denture cleaning [29,30]. The second step in DS is adherence of Candida albicans to host epithelial cells is a critical first step in the infection process [6][7][8]23,[29][30][31][32][33].…”

“…Denture base cracks may become to be one of the best sites for microorganism propagation and provides protection from shear forces, even during denture cleaning [29,30]. The second step in DS is adherence of Candida albicans to host epithelial cells is a critical first step in the infection process [6][7][8]23,[29][30][31][32][33]. It is essential for both colonization and subsequent induction of mucosal disease [31].…”

Biocompatibility of a titanium dioxide‐coating method for denture base acrylic resin

“…However, despite its obvious suitability as a denture base material 2,3 , PMMA is also susceptible to deterioration 4 , surface roughness following fatigue, microbial adherence [5][6][7] and colonisation by bacteria due to water absorption 1 . However, despite its obvious suitability as a denture base material 2,3 , PMMA is also susceptible to deterioration 4 , surface roughness following fatigue, microbial adherence [5][6][7] and colonisation by bacteria due to water absorption 1 .…”

“…Polymethyl methacrylate (PMMA) resin has long been used as a denture base material 1 , because of its hardness and rigidity under masticatory pressure, ease of handling, good aesthetics and low price. However, despite its obvious suitability as a denture base material 2,3 , PMMA is also susceptible to deterioration 4 , surface roughness following fatigue, microbial adherence [5][6][7] and colonisation by bacteria due to water absorption 1 .…”