Effect of biolinker on the detection of prostate specific antigen in an interferometry

Choi, Jung-Min; An, Jin-Young; Kim, Byung-Woo

doi:10.1007/s12257-008-0108-2

Cited by 10 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Prostate cancer is a major cancerous disease in the male population and accounts for about 10% of the deaths from cancers; [17][18][19] hence its early detection can save millions of lives. Monitoring the human Prostate-Specific Antigen (hPSA) level in serum is by far the most commonly used approach [19].…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs)

Zhao

Pan

Ashley

et al. 2014

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ~1.5 GHz and mass sensitivity of 0.015 mg/m 2 (1.5 ng/cm 2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostatespecific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer biomarker used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and biomarker antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1 mg/m 2 , above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1 mg/m 2) or increased steric effect (above 1 mg/m 2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors.

show abstract

Section: Introductionmentioning

confidence: 99%

Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs)

Zhao

Pan

Ashley

et al. 2014

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

show abstract

“…Although these elaborate antibody immobilization approaches can achieve relatively higher antigenbinding efficiencies, most of them need complicated molecular engineering or dedicated laboratory skills, making them limited for widespread, fast and costeffective applications [8]. The current industry prefers to use either direct printing or solution adsorption as efficient means for antibody immobilization.…”

Section: Introductionmentioning

confidence: 99%

Interfacial recognition of human prostate-specific antigen by immobilized monoclonal antibody: effects of solution conditions and surface chemistry

Zhao

Pan

Garcia‐Gancedo

et al. 2012

J. R. Soc. Interface.

View full text Add to dashboard Cite

The specific recognition between monoclonal antibody (anti-human prostate-specific antigen, anti-hPSA) and its antigen (human prostate-specific antigen, hPSA) has promising applications in prostate cancer diagnostics and other biosensor applications. However, because of steric constraints associated with interfacial packing and molecular orientations, the binding efficiency is often very low. In this study, spectroscopic ellipsometry and neutron reflection have been used to investigate how solution pH, salt concentration and surface chemistry affect antibody adsorption and subsequent antigen binding. The adsorbed amount of antibody was found to vary with pH and the maximum adsorption occurred between pH 5 and 6, close to the isoelectric point of the antibody. By contrast, the highest antigen binding efficiency occurred close to the neutral pH. Increasing the ionic strength reduced antibody adsorbed amount at the silica-water interface but had little effect on antigen binding. Further studies of antibody adsorption on hydrophobic C8 (octyltrimethoxysilane) surface and chemical attachment of antibody on (3-mercaptopropyl)trimethoxysilane/4-maleimidobutyric acid N-hydroxysuccinimide ester-modified surface have also been undertaken. It was found that on all surfaces studied, the antibody predominantly adopted the 'flat on' orientation, and antigen-binding capabilities were comparable. The results indicate that antibody immobilization via appropriate physical adsorption can replace elaborate interfacial molecular engineering involving complex covalent attachments.

show abstract

“…Prostate cancer is a major cancerous disease in male population and accounts for about 10% of deaths from cancers. − Its early detection can save millions of lives. Monitoring the prostate-specific antigen (PSA) level in serum is by far the most commonly used approach.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, quantitative detection of fPSA and PSA–ACT can effectively help diagnose prostate cancer. However, the low cutoff limit of the PSA (2.5–4 ng/mL) challenges current detection methods. , Techniques such as surface plasmon resonance (SPR), quartz crystal microbalance (QCM), electrochemical signal transduction, and microcantilever have all been explored for the detection of PSAs. , Many approaches including self-assembled monolayers (SAM), biotinylation, fluorescent labeling, protein linkers or ligands, nanoparticle conjugates, and secondary antibodies have been developed to improve detection sensitivity. ,,− Among these different approaches, however, label-free detection methods are of interest for future biosensor applications. , We have recently demonstrated that neutron reflection (NR) is one of the techniques that can effectively determine antibody molecular orientations at the solid/water interfaces. ,, In this paper, spectroscopic ellipsometry (SE), NR, and dual polarization interferometry (DPI) have been combined to investigate the interfacial assembly of PSA antibody [mouse monoclonal anti-human prostate-specific antigen (anti-hPSA)] at the silicon oxide/water interface and the subsequent in situ antigen binding. The direct determination of in situ interfacial antibody orientation and its relationship to antigen binding is an important step in optimizing biosensor efficiency.…”

Section: Introductionmentioning

confidence: 99%

Interfacial Immobilization of Monoclonal Antibody and Detection of Human Prostate-Specific Antigen

et al. 2011

View full text Add to dashboard Cite

Antibody orientation and its antigen binding efficiency at interface are of particular interest in many immunoassays and biosensor applications. In this paper, spectroscopic ellipsometry (SE), neutron reflection (NR), and dual polarization interferometry (DPI) have been used to investigate interfacial assembly of the antibody [mouse monoclonal anti-human prostate-specific antigen (anti-hPSA)] at the silicon oxide/water interface and subsequent antigen binding. It was found that the mass density of antibody adsorbed at the interface increased with solution concentration and adsorption time while the antigen binding efficiency showed a steady decline with increasing antibody amount at the interface over the concentration range studied. The amount of antigen bound to the interfacial immobilized antibody reached a maximum when the surface-adsorbed amount of antibody was around 1.5 mg/m(2). This phenomenon is well interpreted by the interfacial structural packing or crowding. NR revealed that the Y-shaped antibody laid flat on the interface at low surface mass density with a thickness around 40 Å, equivalent to the short axial length of the antibody molecule. The loose packing of the antibody within this range resulted in better antigen binding efficiency, while the subsequent increase of surface-adsorbed amount led to the crowding or overlapping of antibody fragments, hence reducing the antigen binding due to the steric hindrance. In situ studies of antigen binding by both NR and DPI demonstrated that the antigen inserted into the antibody layer rather than forming an additional layer on the top. Stability assaying revealed that the antibody immobilized at the silica surface remained stable and active over the monitoring period of 4 months. These results are useful in forming a general understanding of antibody interfacial behavior and particularly relevant to the control of their activity and stability in biosensor development.

show abstract

Effect of biolinker on the detection of prostate specific antigen in an interferometry

Cited by 10 publications

References 33 publications

Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs)

Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs)

Interfacial recognition of human prostate-specific antigen by immobilized monoclonal antibody: effects of solution conditions and surface chemistry

Interfacial Immobilization of Monoclonal Antibody and Detection of Human Prostate-Specific Antigen

Contact Info

Product

Resources

About