Effect of biological implant surface coatings on bone formation, applying collagen, proteoglycans, glycosaminoglycans and growth factors

Stadlinger, Bernd; Pilling, Eckart; Mai, Ronald; Bierbaum, Susanne; Berhardt, Ricardo; Scharnweber, Dieter; Eckelt, Uwe

doi:10.1007/s10856-007-3077-7

Cited by 68 publications

(64 citation statements)

References 36 publications

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“…Finally, it should be noted that because the available energy of X-rays is limited to near the zirconium dioxide k-edge, this method would be unsuitable for specimens that need high-energy X-rays far beyond the zirconium dioxide k-edge, including a thick wall of dense compact bone and bone-implant interfaces. 19,20,47 In conclusion, we have established the feasibility of the combined technique of sSRCT and ZrCA vascular casting in quantitative 3D imaging of angiogenesis and bone repair. Its application to early-stage bone repair in rat cortical drill-hole defects could quantify the impact of mechanical stimuli on vascular ingrowth and bone repair, demonstrating the potential of the present imaging method for the enhanced understanding of angiogenesis and its involvement in bone repair.…”

Section: Discussionmentioning

confidence: 88%

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

Matsumoto

Goto

Sato

2013

Laboratory Investigation

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Quantitative three-dimensional (3D) imaging of angiogenesis during bone repair remains an experimental challenge. We developed a novel contrast agent containing 0.07-to 0.1-mm particles of zirconium dioxide (ZrCA) and established subtraction mCT using synchrotron radiation (sSRCT) for quantitative imaging of angiogenesis and bone repair. This method was applied to a rat model of tibial bone repair 3 days (DAY3; n ¼ 2), 5 days (DAY5; n ¼ 8), or 10 days (DAY10; n ¼ 8) after drill-hole injury. Using the same drill-hole defect model, its potential use was illustrated by comparison of bone repair between hindlimbs subjected to mechanical unloading (n ¼ 6) and normal weight bearing (n ¼ 6) for 10 days. Following vascular casting with ZrCA, the defect site was scanned with 17.9-and 18.1-keV X-rays. In the latter, image contrast between ZrCA-filled vasculature and bone was enhanced owing to the sharp absorption jump of zirconium dioxide at 18.0 keV (k-edge). The two scan data sets were reconstructed with 2.74-mm voxel resolution, registered by mutual information, and digitally subtracted to extract the contrast-enhanced vascular image. K 2 HPO 4 phantom solutions were scanned at 17.9 keV for quantitative evaluation of bone mineral. Angiogenesis had already started, but new bone formation was not found on DAY3. New bone emerged near the defect boundary on DAY5 and took the form of trabecular-like structure invaded by microvessels on DAY10. Vascular and bone volume fractions, blood vessel and bone thicknesses, and mineralization were higher on DAY10 than on DAY5. All these parameters were found to be decreased after 10 days of hindlimb unloading, indicating the possible involvement of angiogenesis in bone repair impairment caused by reduced mechanical stimuli. In conclusion, the combined technique of sSRCT and ZrCA vascular casting is suitable for quantitative 3D imaging of angiogenesis and its surrounding bone regeneration. This method will be useful for better understanding the linkage between angiogenesis and bone repair.

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Section: Discussionmentioning

confidence: 88%

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

Matsumoto

Goto

Sato

2013

Laboratory Investigation

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“…(C) Collagen fibers were adsorbed on titanium and grafted with the proteoglycan decorin. Through negatively charged GAG chains of decorin, TGF-β1 (PDB: 1KLC) could be immobilized to increase the bone-implant contact (Stadlinger et al, 2008). Electrostat.…”

Section: Trigger Cell Function In a Bioinert Backgroundmentioning

confidence: 99%

“…Increase of bone-implant contact Stadlinger et al (2008) BMP-2, bone morphogenetic protein; bFGF, basic fibroblast growth factor; OGP, osteogenic growth peptide; VEGF, vascular endothelial growth factor; SDF-1, stromal cell derived factor; TGF, transforming growth factor; PLLA, poly(L-lactide); PGS, poly(glycerol sebacate); PLGA, poly(lactic-co-glycolic acid); CS, chondroitin sulfate; + indicates the combination of certain molecules.…”

Section: Trigger Cell Function In a Bioinert Backgroundmentioning

confidence: 99%

“…Moreover, both complexes were combined in a multifunctional coating. In vivo experiments with functionalized bone implants exhibited a combination of collagen/chondroitin sulfate and BMP-4 as the most promising titanium modification since the bone-implant contact was enhanced the most on this coating (Stadlinger et al, 2008).…”

Section: Multifunctional Coatings To Simultaneously Modulate Diverse mentioning

confidence: 99%

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Multifunctional biomaterial coatings: synthetic challenges and biological activity

Pagel

Beck‐Sickinger

2017

Biological Chemistry

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A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter molecules to improve cell-material contacts, prevent infections or to analyze biochemical events on the surface. However, specific immobilization and particular assembly of various active molecules are challenging. Herein, an overview of multifunctional coatings for biomaterials is given, focusing on synthetic strategies and the biological benefits by displaying several motifs.

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“…Foi demonstrado que esses fatores promovem formação óssea ectópica em vários sistemas, assim como exercem papéis críticos no reparo de fraturas (STADLINGER et al, 2008b;SUTTAPREYASRI et al, 2006;WEI et al, 2007).…”

Section: Samira Salmeronunclassified

Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal

Salmeron¹

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Effect of biological implant surface coatings on bone formation, applying collagen, proteoglycans, glycosaminoglycans and growth factors

Abstract: The results indicate that collagen/CS and collagen/CS/BMP-4 lead to a higher degree of bone formation compared to other ECM components.

Cited by 68 publications

References 36 publications

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

Multifunctional biomaterial coatings: synthetic challenges and biological activity

Efeito da desmineralização óssea sobre parâmetros de superfície e sobre o comportamento de pré-osteoblastos em cultura: estudo em microscopia eletrônica de varredura e confocal

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