Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization

Pathak, Priyadarshini; Singh, Shrawan Kumar; Tandon, Chanderdeep

doi:10.1590/s1677-55382010000500013

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…The antilithiasic potency of various plants like Dolichos biflorus (23), Trachyspermum ammi (24), Tribulus terrestris (2) and Achyranthes aspera (25) and the inhibitory effect of various biomolecules on renal stones (26) have been evaluated in our lab.…”

Section: Discussionmentioning

confidence: 99%

Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells

et al. 2012

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Section: Discussionmentioning

confidence: 99%

Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells

et al. 2012

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“…Protein bands were stained with silver using ProteoSilver™ Plus Silver Stain Kit (PROTSIL2, Sigma-Aldrich Co.). Broad range and low range molecular weight markers (catalog # 161-0317, # 161-0304 Bio-Rad) were used as standards [19].…”

Section: Methodsmentioning

confidence: 99%

Peeping into Human Renal Calcium Oxalate Stone Matrix: Characterization of Novel Proteins Involved in the Intricate Mechanism of Urolithiasis

et al. 2013

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BackgroundThe increasing number of patients suffering from urolithiasis represents one of the major challenges which nephrologists face worldwide today. For enhancing therapeutic outcomes of this disease, the pathogenic basis for the formation of renal stones is the need of hour. Proteins are found as major component in human renal stone matrix and are considered to have a potential role in crystal–membrane interaction, crystal growth and stone formation but their role in urolithiasis still remains obscure.MethodsProteins were isolated from the matrix of human CaOx containing kidney stones. Proteins having MW>3 kDa were subjected to anion exchange chromatography followed by molecular-sieve chromatography. The effect of these purified proteins was tested against CaOx nucleation and growth and on oxalate injured Madin–Darby Canine Kidney (MDCK) renal epithelial cells for their activity. Proteins were identified by Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF MS) followed by database search with MASCOT server. In silico molecular interaction studies with CaOx crystals were also investigated.ResultsFive proteins were identified from the matrix of calcium oxalate kidney stones by MALDI-TOF MS followed by database search with MASCOT server with the competence to control the stone formation process. Out of which two proteins were promoters, two were inhibitors and one protein had a dual activity of both inhibition and promotion towards CaOx nucleation and growth. Further molecular modelling calculations revealed the mode of interaction of these proteins with CaOx at the molecular level.ConclusionsWe identified and characterized Ethanolamine-phosphate cytidylyltransferase, Ras GTPase-activating-like protein, UDP-glucose:glycoprotein glucosyltransferase 2, RIMS-binding protein 3A, Macrophage-capping protein as novel proteins from the matrix of human calcium oxalate stone which play a critical role in kidney stone formation. Thus, these proteins having potential to modulate calcium oxalate crystallization will throw light on understanding and controlling urolithiasis in humans.

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“…Later they investigated the activity of high and low molecular weight biomolecules present in the matrix of human CaOx stones not only on the initial mineral phase formation of calcium and phosphate (CaP) but also on its growth and demineralization of the preformed mineral phase [226]. We also purified, identified, and characterized novel proteins from human renal stone matrix.…”

Section: Matrix Componentsmentioning

confidence: 99%

Nephrolithiasis: Molecular Mechanism of Renal Stone Formation and the Critical Role Played by Modulators

Aggarwal

Narula

Kakkar³

et al. 2013

BioMed Research International

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Urinary stone disease is an ailment that has afflicted human kind for many centuries. Nephrolithiasis is a significant clinical problem in everyday practice with a subsequent burden for the health system. Nephrolithiasis remains a chronic disease and our fundamental understanding of the pathogenesis of stones as well as their prevention and cure still remains rudimentary. Regardless of the fact that supersaturation of stone-forming salts in urine is essential, abundance of these salts by itself will not always result in stone formation. The pathogenesis of calcium oxalate stone formation is a multistep process and essentially includes nucleation, crystal growth, crystal aggregation, and crystal retention. Various substances in the body have an effect on one or more of the above stone-forming processes, thereby influencing a person's ability to promote or prevent stone formation. Promoters facilitate the stone formation while inhibitors prevent it. Besides low urine volume and low urine pH, high calcium, sodium, oxalate and urate are also known to promote calcium oxalate stone formation. Many inorganic (citrate, magnesium) and organic substances (nephrocalcin, urinary prothrombin fragment-1, osteopontin) are known to inhibit stone formation. This review presents a comprehensive account of the mechanism of renal stone formation and the role of inhibitors/promoters in calcium oxalate crystallisation.

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Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization

Cited by 8 publications

References 26 publications

Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells

Cytoprotective role of the aqueous extract of Terminalia chebula on renal epithelial cells

Peeping into Human Renal Calcium Oxalate Stone Matrix: Characterization of Novel Proteins Involved in the Intricate Mechanism of Urolithiasis

Nephrolithiasis: Molecular Mechanism of Renal Stone Formation and the Critical Role Played by Modulators

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