Effect of biopolymers containing natamycin against Aspergillus niger and Penicillium roquefortii on fresh kashar cheese

Abstract

SummaryFungal spoilage during refrigerated storage is one of the main safety and quality-related problems for dairy products. The effect of wheat gluten (WG) and methyl cellulose (MC) biopolymers containing natamycin (NA) on the growth of Aspergillus niger and Penicillium roquefortii on the surface of fresh kashar cheese during storage at 10°C for 30 days was investigated. Wrapping of A. niger-inoculated cheese with MC films containing 5-20 mg NA per 10 g resulted in approximately 2-log reductions in spore count. Two mg NA per 10 g included into WG films was sufficient to eliminate A. niger on the surface of cheese. However, MC and WG films containing NA did not cause any significant decrease in P. roquefortii count on the cheese surface. Therefore, especially use WG films in dairy applications could be an effective way of controlling A. niger growth on these products.

Expression of Paenibacillus polymyxa β-1,3-1,4-glucanase in Streptomyces lydicus A01 improves its biocontrol effect against Botrytis cinerea

“…Streptomyces lydicus strain A01 isolated from the soil of suburban vegetable field in Beijing, China, is capable of producing natamycin and chitinase, and has a significant inhibition effect on B. cinerea (Lu et al, 2008;Wu et al, 2013a,b). Natamycin is a tetraene macrolide antibiotic widely used as a food preservative and fungicide in food, medicine, and veterinary products, and it has the potential to control plant fungal diseases in agricultural production (Ture et al, 2011;Currie et al, 1990;Lu et al, 2008;Wu et al, 2014a). Pathogenesis-related (PR) proteins such as chitinases and glucanases can degrade fungal cell walls and play important roles in plant defence against pathogens, and are widely used as antifungal agents in plant protection (Errakhi et al, 2007;Shi et al, 2010;Park et al, 2012).…”

Heterologous coexpression of Vitreoscilla hemoglobin and Bacillus megaterium glucanase in Streptomyces lydicus A02 enhanced its production of antifungal metabolites

“…The antifungal activity of S. lydicus A02 is mainly due to the production of natamycin, a tetraene macrolide antibiotic widely used as a food preservative and fungicide, with potential application for plant fungal disease control in agricultural production [7][8][9][10]. Glucanases and chitinases are pathogenesisrelated (PR) proteins; they can degrade fungal cell walls and have been widely used as antifungal agents in plant protection [11,12].…”

Smart Nanohydrogels for Controlled Release of Food Preservatives

“…PIMARICIN 17 economical losses and health problems owing to mycotoxins [219]. Pimaricin is normally applied in these products as an aqueous suspension by spraying the surface or soaking the cheese in a solution of pimaricin [192].…”

“…For example, chitosan-based edible films [59], wheat gluten and methyl cellulose biopolymers [192,191] and polyethylene films coated with commercially available polyvinylchloride (PVC) lacquer coatings [81], whey protein films [148], alginate-based coatings [134], cellulosebased films [136].…”