Effect of biotic and abiotic elicitors on production of betulin and betulinic acid in the hairy root culture of <i>Betula pendula</i> Roth

Hajati, Razieh Jafari; Payamnoor, Vahide; Chashmi, Najmeh Ahmadian

doi:10.1080/10826068.2019.1650372

Cited by 7 publications

(1 citation statement)

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“…Advancements in biotechnological methods of enhancement of biosynthesis of specialized metabolites in plant in vitro cultures throughout the last couple decades have enabled research to combine increased productivity with minimized effects exerted on general metabolism. Thus, recent reports concerning the triterpenoid saponin productivity in various cell, tissue, and organ cultures of plants described in this review often do not contain the analysis of sterol content [ 84 , 85 , 86 , 87 ], since the phenomenon of their competition with triterpenoid pathway can be neglected after successful application of a suitable strategy.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Phytosterol and Triterpenoid Production in Plant Hairy Root Cultures—Simultaneous Stimulation or Competition?

Rogowska

Szakiel

2021

Plants

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Plant in vitro cultures, including hairy roots, can be applied for controlled production of valuable natural products, such as triterpenoids and sterols. These compounds originate from the common precursor squalene. Sterols and triterpenoids distinctly differ in their functions, and the 2,3-oxidosqualene cyclization step is often regarded as a branch point between primary and secondary (more aptly: general and specialized) metabolism. Considering the crucial role of phytosterols as membrane constituents, it has been postulated that unconstrained biosynthesis of triterpenoids can occur when sterol formation is already satisfied, and these compounds are no longer needed for cell growth and division. This hypothesis seems to follow directly the growth-defense trade-off plant dilemma. In this review, we present some examples illustrating the specific interplay between the two divergent pathways for sterol and triterpenoid biosynthesis appearing in root cultures. These studies were significant for revealing the steps of the biosynthetic pathway, understanding the role of particular enzymes, and discovering the possibility of gene regulation. Currently, hairy roots of many plant species can be considered not only as an efficient tool for production of phytochemicals, but also as suitable experimental models for investigations on regulatory mechanisms of plant metabolism.

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