Effect of Biotin Deficiency on the Synthesis of Nucleic Acids and Protein by Saccharomyces cerevisiae

Change of Cell Wall Polysaccharides of Yeast in Biotin Deficiency

“…AHMAD et al (10) pointed out certain changes in chemical composition of cells of S, cerevisiae due to biotin deficiency, and GHOSH et al (11) reported cell aggregation of S. carlsbergensis caused by inositol deficiency which brought about an enrichment of glucan but not of mannan.…”

A specific requirement for biotin in the synthesis of ornithine carbamoyltransferase by yeast

“…(i) ,B-Fructofuranosidase (P-D-fructofuranoside fructohydrolyase; EC 3.2.1.26) activity of whole yeast cells was SYNTHESIS IN YEAST 515 determined at 370 by the method described by Ahmad & Rose (1962 Ahmad, Rose & Garg (1961). Protein in the residue after acid-soluble u.v.-absorbing compounds, RNA and DNA had been extracted from the yeast was estimated by the micro-Kjeldahl method (Markham, 1942) with an HgO catalyst (Miller & Houghton, 1954).…”

Observations on the Fine Structure of Saccharomyces cerevisiae as Affected by Biotin Deficiency

“…The yeast was grown in the chemically defined medium of Rose & Nickerson (1956) using the techniques described by Ahmad et al (1961). The medium contained either an optimal (8.0 x 1 0 -l O~) or a, suboptimal (0.4 x 10-lO~) concentration of D-biotin.…”

“…These changes include a decreased synthesis of nucleic acids and total protein as compared with biotin-optimal yeast (Ahmad, Rose & Garg, 1961), and marked alterations in the amounts of certain enzymes synthesized by the yeast (Ahmad & Rose, 1 9 6 2~) .…”