Effect of Biotin on Fatty Acid Distribution in
            <i>Escherichia coli</i>

Gavin, John J.; Umbreit, W. W.

doi:10.1128/jb.89.2.437-443.1965

Cited by 24 publications

(7 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our relative retention data agree closely with those of Kaneshiro and Marr (14), who used a column containing 25% DEGS at a temperature of 187 C. Although the percentage of cyclic C17 in E. coli appears to agree closely with previously published results, we found a much larger amount (18 to 19%) of lactobacillic acid than that reported by other workers (6,7,14,21). Our cyclopropane acid content for S. marcescens agrees closely with Kates and Hagen (17), Kates, Adams, and Martin (16), and Bishop and Still (2).…”

Section: Discussionsupporting

confidence: 92%

A Simple Procedure for Detecting the Presence of Cyclopropane Fatty Acids in Bacterial Lipids

Brian

Gardner

1968

Applied Microbiology

View full text Add to dashboard Cite

Four gram-negative bacterial species, including Escherichia coli strain B, Serratia marcescens, Pseudomonasfluorescens, and Vibrio cholerae (comma) strain NIH 41, were investigated for fatty acid content by gas-liquid chromatography involving a preparatory technique which facilitated detection of cyclopropane fatty acids. Methyl esters of fatty acids were subjected to mild catalytic hydrogenation to eliminate unsaturates. Hydrogenation was followed by bromination which removed cyclopropane acids from chromatographic profile patterns. Lactobacillic acid (cis-11,12-methyleneoctanoate) and cis-9,10-methylenehexadecanoate, previously reported in lipids of E. coli and S. marcescens, were found in small amounts in P. fluorescens but were not detected in V. cholerae.

show abstract

Section: Discussionsupporting

confidence: 92%

A Simple Procedure for Detecting the Presence of Cyclopropane Fatty Acids in Bacterial Lipids

Brian

Gardner

1968

Applied Microbiology

View full text Add to dashboard Cite

show abstract

“…By using other biotin-requiring strains or mutants, the C18 monoenoic acids have been shown to decrease in biotin deficiency (1,5). Biotin is required for de novo synthesis of fatty acids (20).…”

Section: Discussionmentioning

confidence: 99%

Effect of Biotin on Fatty Acids and Phospholipids of Biotin-Sensitive Strains of Rhizobium japonicum

1970

View full text Add to dashboard Cite

The effect of biotin on fatty acids and intact lipids was studied by comparing a biotin-requiring, a biotin-inhibited, and a biotin-indifferent strain of Rhizobium japonicum. These organisms were grown in a defined medium with added levels of 0, 0.3, and 0.5 ,ug of biotin per liter, and were analyzed for fatty acids and lipid components. Myristic, palmitic, and octadecenoic acids were found to be the major fatty acids in these strains. The indifferent strain also contained large amounts of C1, cyclopropane acid and small amounts of a C17 cyclopropane acid. Several unidentified acids were present in the other two strains. The percentages of fatty acids showed statistically significant changes corresponding with changes in level of biotin in the medium. When biotin concentration was increased in the medium, the Cis monoenoic acids of the biotin-requiring strain increased significantly, and those of the biotin-inhibited and'biotin-indifferent strains decreased significantly. Palmitic acid showed a statistically significant increase in the indifferent strain with increasing biotin concentration. The principal intact lipid components in these strains are phospholipids. The major phospholipids are phosphatidylserine, phosphatidylcholine, phosphatitidylethanolamine, and cardiolipin. These phospholipids were not affected by biotin level and were independent of medium composition. microbiological assay by using Lactobacillus plantarum 8014. The growth response of L. plantarum

show abstract

“…The addition of minute quantities of biotin to the culture medium has had a demonstrable effect on the fatty acid composition of various organisms (3,4,9,20,21,35). The effect of biotin on the fatty acid spectrum of both autotrophically and heterotrophically grown cultures of T. novellus was determined.…”

mentioning

confidence: 99%

Effect of Cultural Conditions on the Fatty Acid Composition of Thiobacillus novellus

Levin

1972

J Bacteriol

View full text Add to dashboard Cite

The fatty acid content of Thiobacillus novellus was determined under various cultural conditions. Four fatty acids, C:e0, C 18: o C 18: , and a C cyclopropane acid (C19:cyc), generally accounted for 90 to 99% of the total acids. Phosphate concentration, temperature, culture agitation, and the presence of branch-chain precursors had no significant effect on cellular fatty acids. Autotrophically grown cells contained more saturated C,, and C18 acids than did heterotrophic ones, and the sum of the percentages of the C 181 and the C i9 cyc acids was consistently higher in the heterotrophs. When the inorganic medium was supplemented with biotin, autotrophic cells produced more C,9:yC and

show abstract

Effect of Biotin on Fatty Acid Distribution in Escherichia coli

Cited by 24 publications

References 18 publications

A Simple Procedure for Detecting the Presence of Cyclopropane Fatty Acids in Bacterial Lipids

A Simple Procedure for Detecting the Presence of Cyclopropane Fatty Acids in Bacterial Lipids

Effect of Biotin on Fatty Acids and Phospholipids of Biotin-Sensitive Strains of Rhizobium japonicum

Effect of Cultural Conditions on the Fatty Acid Composition of Thiobacillus novellus

Contact Info

Product

Resources

About