Effect of Bis(maltolato)oxovanadium(IV) on Zinc, Copper, and Manganese Homeostasis and DMT1 mRNA Expression in Streptozotocin-Induced Hyperglycemic Rats

Abstract

Our aim was to examine whether vanadium (IV) corrects alterations in zinc, copper and manganese homeostasis, observed in streptozotocin-induced hyperglycemic rats, and whether such changes are related to divalent metal transporter 1 (DMT1) mRNA expression, and antioxidant and proinflammatory parameters. Four groups of Wistar rats were examined: control; hyperglycemic (H); hyperglycemic treated with 1 mg V/day (HV); and hyperglycemic treated with 3 mg V/day (HVH). Vanadium was supplied in drinking water as bis(maltolato)oxovanadium(IV) for five weeks. Zinc, copper and manganese were measured in food, excreta, serum and tissues. DMT1 mRNA expression was quantified in the liver. Hyperglycemic rats showed increased Zn and Cu absorption and content in the liver, serum, kidneys and femurs; DMT1 expression also increased (p < 0.05 in all cases). HV rats showed no changes compared to H rats other than decreased DMT1 expression (p < 0.05). In the HVH group, decreased absorption and tissular content of studied elements (p < 0.05 in all cases) and DMT1 expression compared to H (p < 0.05) were observed. Liver zinc, copper and manganese content correlated positively with glutathione peroxidase activity and negatively with catalase activity (p < 0.05 in both cases). In conclusion, treatment with 3 mg V/d reverted the alterations in zinc and copper homeostasis caused by hyperglycemia, possibly facilitated by decreased DMT1 expression.

Elucidating the Therapeutic Potential of Bis(Maltolato)OxoVanadium(IV): The Protective Role of Copper in Cellular Metabolism

“…This same effect has been previously described because BMOV exposure increased the uptake of another antioxidant metal (Mn) [11]. These results are consistent with others in which only increases in Cu have been found in the kidneys of rats treated with V [30]. The co-exposure of hepatocytes to both metals led to repetition in the trends for individual treatments.…”

Single and Combined Associations of Plasma and Urine Essential Trace Elements (Zn, Cu, Se, and Mn) with Cardiovascular Risk Factors in a Mediterranean Population

“…Single-element standard solution for ICP-MS containing 1.000 µg/mL of Cu, Zn, Mn, and Se (Merck & Co. Inc., Whitehouse Station, NJ, USA). The plasma samples were previously prepared with a basic solution containing ammonium hydroxide, butanol, EDTA, and triton X-100 [ 108 , 109 ]. Urine samples were previously prepared with an acidic solution containing 1% NHO 3 and 0.5% HCl.…”

Metals and Metal Complexes in Diseases with a Focus on COVID-19: Facts and Opinions

“…The original articles focused on (1) the assessment of the potential biomedical use of polyoxotungstates (POTs) [ 14 ], (2) the influence of vanadium (V) on the homeostasis of some minerals and divalent metal transporter 1 (DMT1) mRNA expression in streptozotocin-induced hyperglycemic rats [ 15 ], and (3) the in vivo efficiency and safety of long-term administration of clinoptilolite in its activated forms [ 16 ], i.e., tribomechanically activated zeolite (TMAZ) and panaceo micro-activated zeolite (PMAZ) on the biodistribution of such metals as Al, As, Cd, Co, Pb, Ni, and Sr.…”

“…The results obtained by Sánchez-González et al [ 15 ] show that diabetes alters the metabolism of such minerals as Zn and Cu and, to a lesser degree, Mn, and that bis(maltolato)oxovanadium (IV), BMOV, administered to diabetic rats as a hypoglycemic agent at a dose of 3 mg V/day reverts the changes in Zn and Cu homeostasis caused by this disease. The authors suggest that the effect of supplementation with BMOV is possibly mediated by a fall in food intake and DMT1 gene expression.…”

“…The authors suggest that the effect of supplementation with BMOV is possibly mediated by a fall in food intake and DMT1 gene expression. As stressed, more studies are needed to better determine the mode of action responsible for the observed changes, recognize the effects of the interactions, and evaluate the optimum level of pharmacological intervention in order to avoid or prevent side effects [ 15 ].…”