Effect of blood sample handling post-collection on Erysipelothrix rhusiopathiae antibody titres

Neumann, E.; Bonistalli, Kathryn N.

doi:10.1016/j.tvjl.2008.07.020

Cited by 21 publications

(14 citation statements)

References 4 publications

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“…To circumvent this problem, many recent brucellosis studies in wildlife have been based on immunosorbent assays -ELISA- [17,41,42]. One of the advantages of this serological test is that the degree of haemolysis of the serum samples does not affect significantly the ELISA performance [43]. Due to the absence of specific conjugates against the immunoglobulin isotypes of the different wildlife species, indirect ELISAs have not been widely used, and most of studies have been based on the use of competitive ELISAs, which are potentially able to identify specific anti- Brucella antibodies in all animal species [28,44-46].…”

Section: Discussionmentioning

confidence: 99%

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

et al. 2010

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BackgroundThe role of wildlife as a brucellosis reservoir for humans and domestic livestock remains to be properly established. The aim of this work was to determine the aetiology, apparent prevalence, spatial distribution and risk factors for brucellosis transmission in several Iberian wild ungulates.MethodsA multi-species indirect immunosorbent assay (iELISA) using Brucella S-LPS antigen was developed. In several regions having brucellosis in livestock, individual serum samples were taken between 1999 and 2009 from 2,579 wild bovids, 6,448 wild cervids and4,454 Eurasian wild boar (Sus scrofa), and tested to assess brucellosis apparent prevalence. Strains isolated from wild boar were characterized to identify the presence of markers shared with the strains isolated from domestic pigs.ResultsMean apparent prevalence below 0.5% was identified in chamois (Rupicapra pyrenaica), Iberian wild goat (Capra pyrenaica), and red deer (Cervus elaphus). Roe deer (Capreolus capreolus), fallow deer (Dama dama), mouflon (Ovis aries) and Barbary sheep (Ammotragus lervia) tested were seronegative. Only one red deer and one Iberian wild goat resulted positive in culture, isolating B. abortus biovar 1 and B. melitensis biovar 1, respectively. Apparent prevalence in wild boar ranged from 25% to 46% in the different regions studied, with the highest figures detected in South-Central Spain. The probability of wild boar being positive in the iELISA was also affected by age, age-by-sex interaction, sampling month, and the density of outdoor domestic pigs. A total of 104 bacterial isolates were obtained from wild boar, being all identified as B. suis biovar 2. DNA polymorphisms were similar to those found in domestic pigs.ConclusionsIn conclusion, brucellosis in wild boar is widespread in the Iberian Peninsula, thus representing an important threat for domestic pigs. By contrast, wild ruminants were not identified as a significant brucellosis reservoir for livestock.

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Section: Discussionmentioning

confidence: 99%

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

et al. 2010

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“…Long-term storage of serum samples at Ϫ20°C has been shown to decrease measured immunoglobulin G concentrations (26). However, storage at Ϫ80°C, as was done in this study, is considered the standard for samples to be assayed for immunoglobulin concentration and has not been associated with a decrease in immunoglobulin concentrations in other species (27,28); therefore, it is unlikely that this affected antibody concentrations in our study.…”

Section: Discussionmentioning

confidence: 74%

Antibody Responses to Natural Rattlesnake Envenomation and a Rattlesnake Toxoid Vaccine in Horses

Gilliam¹,

Carmichael²,

Holbrook³

et al. 2013

Clin. Vaccine Immunol.

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e Antivenom antibody titers following administration of rattlesnake venom for antivenom production in horses are well documented; however, antivenom antibody titers following natural rattlesnake envenomation in horses are not. Antibody titers produced in response to the commercially available rattlesnake venom vaccine are also not published. Our study objectives were to measure antivenom antibody titers in rattlesnake-bitten horses and compare them to titers in horses vaccinated with the rattlesnake venom vaccine. Additionally, titers were compared in pregnant versus nonpregnant horses to assess the affect of pregnancy on vaccine response and were measured pre-and postsuckle in foals of vaccinated mares to detect passive transfer of vaccine immunoglobulins. Blood samples were collected from16 rattlesnake-bitten horses. Thirty-six horses (11 pregnant mares, 12 nonpregnant mares, 13 geldings) were vaccinated using a Crotalus atrox venom toxoid vaccine. Blood was collected before administering each vaccination and 30 days following the third vaccination. Blood was collected from foals of vaccinated mares pre-and postsuckle. All serum was assayed for anti-Crotalus atrox venom antibodies using an enzyme-linked immunosorbent assay (ELISA). Rattlesnake-bitten horses had higher (P ‫؍‬ 0.001) titers than vaccinated horses. There was no significant difference between titers in vaccinated pregnant versus nonpregnant horses. One mare had a positive titer at foaling, and the foals had positive postsuckle titers. Antivenom antibody titer development was variable following natural envenomation and vaccination, and vaccine-induced titers were lower than natural envenomation titers. Further studies are required to determine if natural or vaccine antivenom antibody titers reduce the effects of envenomation.

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“…However, the sampling in hunted species presents some drawbacks, the volume of samples obtained is often low, and the quality is influenced by different factors which may degrade the blood (Neumann and Bonistalli 2009). The IV method allows the collection of blood directly from the sinus cavernous, taking advantage of the anatomical morphology of the wild boar head bones and the blood volume contained within the venous sinuses.…”

Section: Discussionmentioning

confidence: 99%

Blood sampling by puncture in the cavernous sinus from hunted wild boar

et al. 2013

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A new method of sampling based on the extraction of blood from the cavernous sinus of the dura mater has been assessed in hunted wild boar. Blood from 139 animals was obtained by two different extraction methods: the harvesting from thoracic cavity (TC) and intracavernous venipuncture (IV). Sera obtained by the IV method had higher volume (mean 2.85 vs 1.85 ml), were less hemolytic (mean absorbance at 450 nm: 1.01 vs 2.41 nm). A higher number of samples and a higher proportion of sera collected by IV (90.6 %) compared to those obtained using the TC method (78.4 %), could be analyzed against Aujeszky's disease using blocking ELISA. No statistically significant differences in seroprevalences between samples obtained using both extraction methods were observed. The results obtained indicate that the IV is an easy, fast, reliable, clean, and safe method to collect blood samples from hunted wild boar, proving a real alternative to the traditional collection method.

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Effect of blood sample handling post-collection on Erysipelothrix rhusiopathiae antibody titres

Cited by 21 publications

References 4 publications

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

Spatial distribution and risk factors of Brucellosis in Iberian wild ungulates

Antibody Responses to Natural Rattlesnake Envenomation and a Rattlesnake Toxoid Vaccine in Horses

Blood sampling by puncture in the cavernous sinus from hunted wild boar

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