Effect of Blood Storage on Complete Biochemistry

Verma, Monica; Dahiya, Kiran

doi:10.4172/2155-9864.1000329

Cited by 23 publications

(30 citation statements)

References 17 publications

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“…Sodium concentration in stored PRBCs is variable depending upon whether it is whole blood (>150 meq/L) or PRBCs (>130 meq/L) and duration of its storage (11,19). Obligatory addition of sodium bicarbonate further increases sodium concentration of the final prime (5).…”

Section: Discussionmentioning

confidence: 99%

Hemodiafiltration—A Technique for Physiological Correction of Priming Solution in Pediatric Cardiac Surgery: An In Vitro Study

et al. 2016

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Pediatric cardiopulmonary bypass (CPB) circuit invariably requires priming with packed red blood cells (PRBCs). Metabolic composition of stored PRBCs is unphysiological and becomes worse with increasing duration of storage. It is recommended to correct these abnormalities before initiation of CPB. We tested the hypothesis that hemodiafiltration of the prime with 0.45% saline is sufficient for reducing the metabolic load and reaching a physiologic state. In an in vitro study, 100 mL of blood each from 45 units of PRBCs stored for 3–20 days were used for priming the 45 neonatal CPB circuits. Based upon the method used for removal of excess crystalloid from the prime, circuits were divided into three groups. Group 1: Direct removal through manifold line. Group 2: Ultrafiltration of prime. Group 3: Hemodiafiltration of the prime. Blood gas analyses were obtained from the PRBCs and from the prime before and after removal of crystalloid. Both direct removal of crystalloid and ultrafiltration resulted in significant reduction in biochemical and metabolic load of blood (P < 0.001). However, the final composition of the prime was far from being physiological. Hemodiafiltration resulted in improvement of metabolic parameters to near physiological range (lactate: 33.8 ± 4.44 vs. 14 ± 2.53 mg/dL, pH: 7.05 ± 0.15 vs. 7.34 ± 0.06, bicarbonates: 4.83 ± 0.59 vs. 27.6 ± 2.94 meq/L; P < 0.001). Similarly, sodium (147.76 ± 12.73 vs. 144.6 ± 5.96 meq/L) and potassium (9.6 ± 2.83 vs. 4.23 ± 0.37 meq/L) also changed significantly (P < 0.001) to near physiologic range. Hemodiafiltraion of final prime is a simple, efficients and rapid method of correcting the biochemical parameters and reducing the metabolic load of stored PRBCs towards the physiological range before initiating the CPB.

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Section: Discussionmentioning

confidence: 99%

Hemodiafiltration—A Technique for Physiological Correction of Priming Solution in Pediatric Cardiac Surgery: An In Vitro Study

et al. 2016

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“…In human medicine, numerous, though not always concordant, studies detail the effects of different storage conditions for sample analysis . However, in veterinary medicine, the number of studies examining these effects is limited.…”

Section: Introductionmentioning

confidence: 99%

Serum biochemistry of the Arrau turtle (Podocnemis expansa): Frozen analyte stability and the effects of long‐term storage

Camargo

Abrão

Queiroz³

et al. 2020

Veterinary Clinical Pathol

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Background Turtles are a major source of protein for riverside human populations in Brazil. The encouragement of commercial breeding meets conservation efforts for these animals, and it is, therefore, crucial to understand the physiologic and behavioral aspects of semi‐aquatic species in captive conditions. Serum biochemical tests are ancillary diagnostic tools, and sample storage is a main problem since clinical laboratories are not always available near the habitats of these species. Objectives The aim of this study was to provide information about the stability of albumin, aspartate aminotransferase (AST), calcium, creatinine kinase (CK), total cholesterol (Chol), alkaline phosphatase (ALP), gamma glutamyltransferase (GGT), total protein (TP), and urea at different storage times. Methods In all, 17 Arrau turtles (Podocnemis expansa) were used, and the serum obtained was separated into aliquots and analyzed at 0, 4, 8, 16, and 32 days after being stored at −20°C. Results The results showed that albumin, AST, CK, GGT, and TP suffered interference due to the long storage times. Conclusion Analytes such as ALP, calcium, Chol, and urea can be evaluated for up to 1 month after freezing. Albumin, AST, and TP can be analyzed up to 1 week after freezing without alterations, and CK GGT are best evaluated on fresh samples.

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“…Whole blood degrades progressively during the weeks of refrigerated storage [1]. Hematological, biochemical, and physical properties of whole blood are adversely affected during storage by preservatives used, storage temperature, containers used, and frequency of gentle mixing during storage [2].…”

Section: Introductionmentioning

confidence: 99%

“…These preservative solutions such as citrate phosphate dextrose adenine-1 (CPDA-1) are often contained in blood bags meant for the collection of blood for transfusion. It has been documented that blood stored in CPDA-1 anticoagulated bags remained stable for up to 35 days [1,3]. Sample stability is defined as the capability of blood to retain the initial value of a measured quantity for a defined period within specific limits when stored under defined conditions [3].…”

Section: Introductionmentioning

confidence: 99%

Stability of hematological parameters of canine blood samples stored with citrate phosphate dextrose adenine-1 anticoagulated plastic vacutainers

2019

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Aim: The effect of citrate phosphate dextrose adenine-1 on the hematological parameters of stored Nigerian indigenous dog's blood with plastic vacutainer was investigated. This was done in view of determining the viability and stability of the studied parameters for blood banking purpose. Till date, there is no literature on the stability of whole blood of Nigerian indigenous dogs for blood banking purposes. Materials and Methods: A total of six apparently adult healthy dogs were sampled, and their blood was stored at 4°C and analyzed for their packed cell volume (PCV), hemoglobin (Hgb) concentration, red blood cell (RBC) count, total and differential white blood cell (WBC) count, platelet count (PC), mean corpuscular values (mean corpuscular Hgb [MCH], MCH concentration, and mean corpuscular volume [MCV]), blood potency of hydrogen (pH), and erythrocyte sedimentation rate (ESR) over a period of 14 days. Results: Significant changes were observed in some of the studied parameters. Of the 14 days' study period, PCV, Hgb concentration, total WBC count, PC, and neutrophil count showed no significant (p≥0.05) difference until day 2 post-storage (PS), while RBC count, ESR, MCV, and lymphocyte count showed no significant (p≥0.05) variation up until day 3 PS. Blood pH showed no significant (p≥0.05) variation within 24-h PS but was significantly (p≤0.05) higher than obtained values on days 1, 3, 7, 10, and 14 PS. Conclusion: Based on our finding, we could suggest that hematological laboratories and hemotherapists could use canine blood stored in a plastic vacutainer for 2-3 days.

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Effect of Blood Storage on Complete Biochemistry

Abstract: Background: Prolonged storage of blood leads to alteration in RBCs biochemistry which may lose viability with time.

Cited by 23 publications

References 17 publications

Hemodiafiltration—A Technique for Physiological Correction of Priming Solution in Pediatric Cardiac Surgery: An In Vitro Study

Hemodiafiltration—A Technique for Physiological Correction of Priming Solution in Pediatric Cardiac Surgery: An In Vitro Study

Serum biochemistry of the Arrau turtle (Podocnemis expansa): Frozen analyte stability and the effects of long‐term storage

Stability of hematological parameters of canine blood samples stored with citrate phosphate dextrose adenine-1 anticoagulated plastic vacutainers

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