Effect of body weight on osteopenia in ovariectomized rats

Tj, Wronski; Pa, Schenck; Cintrón, Miriam; Cc, Walsh

doi:10.1007/bf02555700

Cited by 236 publications

(121 citation statements)

References 15 publications

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“…However, it could be suggested that food intake decreased during the first days after osteotomy and then rose recovering to its level (from 8 to 15 g/rat per day in average respectively). This assumption is supported by other results showing that food intake of the intact and Ovx Sprague-Dawley rats of 3 to 6 months of age ranged from 16 to 18 g/rat per day (Wronski et al 1987, Vortherms 2006.…”

Section: Animal Modelsupporting

confidence: 64%

Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period

Komrakova¹,

Werner²,

Wicke³

et al. 2009

Journal of Endocrinology

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The effect of daidzein (D), 4-methylbenzylidene camphor (4-MBC) or estradiol-17b-benzoate (E 2 ) on muscle of osteoporotic rats during fracture healing was studied. After performing a metaphyseal tibia osteotomy in 96 osteoporotic 5-month-old female Sprague-Dawley rats, they received daily 50 mg D, 200 mg 4-MBC or 0 . 4 mg E 2 per kg body weight, or soy free (SF) diet up to 36 and 72 days. Mitochondrial activity, fiber area, and capillary density were analyzed in M. gastrocnemius. Osseous callus bridging of fracture was observed in half of the rats after 36 days. By day 72, fracture was healed in most of the animals. State 3 mitochondrial respiration significantly enhanced in E 2 , 4-MBC and D groups versus SF after 36 days (30, 32 and 32 vs 23 pmol O 2 /s per mg). It declined after 72 days, however, in E 2 group it was still at a higher level versus SF (25, 23 and 21 vs 20 pmol O 2 /s per mg). Size of fast oxidative glycolytic (FOG) and fast glycolytic (FG) fibers, capillary density did not differ significantly between the groups, however, at day 36 an increase in D and 4-MBC groups was detectable. FOG diameter was 64, 66, 68, and 58 mm and FG diameter was 88, 98, 95, and 89 mm in SF, D, 4-MBC, and E 2 groups. The ratio of capillaries to muscle fiber was 1 . 1, 1 . 4, 1 . 3, and 1 . 1 in SF, D, 4-MBC and E 2 groups by day 36. D and 4-MBC react similar to estrogen thereby improving oxidative cell metabolism in severe osteoporotic rats. The level of mitochondrial activity was higher, though no significant morphological differences could be shown.

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Section: Animal Modelsupporting

confidence: 64%

Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period

Komrakova¹,

Werner²,

Wicke³

et al. 2009

Journal of Endocrinology

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“…Much evidence has shown that BMD decreases with increasing age in men, and is accompanied by decreasing sex hormones, but more moderately than in post-menopausal women [5,10,17,18,23,24]. According to previous studies, the high BMD or calcium content of the first and fourth lumbar vertebrae, femur, and tibia coincided with a high testosterone level at the age of 12 months in male Wistar rats [11,14,17].…”

Section: Discussionmentioning

confidence: 97%

Age-Related Changes in Bone Mineral Density, Cross-Sectional Area and Strength at Different Skeletal Sites in Male Rats.

Iida¹,

Fukuda²

2002

J. Vet. Med. Sci.

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ABSTRACT. Age-related changes in bone mineral density (BMD), cross-sectional area and strength strain index (SSI) of the long bones in the limbs and first lumbar vertebra of male Wistar rats were measured by a peripheral quantitative computed tomography (pQCT) method. One hundred and ten rats aged 2-30 months were used. The results indicate that the total (cortical + trabecular), cortical and trabecular BMD values of the metaphysis and cortical BMD values of the diaphysis in the long bones varied for each bone and differed from those of the first lumbar vertebra. The total BMD of long bones showed high values at 6-21 months and then decreased, but these did not always coincide with cortical and trabecular BMD. The values of SSI in the long bones varied. The values of total and cortical BMD and SSI of lumbar vertebra increased for 6-12 months and then decreased, but the trabecular BMD increased after 12 months. The total area in both the long bones and the first lumbar vertebra increased with the decrease in cortical area and the increase in the trabecular area with increasing age. It was concluded that age-related changes in bones, similar to those observed in humans, could be observed in some bones and parameters, although the age in rats when the so-called peak bone mass appears in the whole skeleton could not be clearly determined. KEY WORDS: age change, bone mineral density, male rat, peak bone mass, peripheral quantitative computed tomography (pQCT) J. Vet. Med. Sci. 64(1): 29-34, 2002 Rats are widely used for studies on bone metabolism and diseases such as osteoporosis, although their worth as an animal model has been contested [6,12,16,21] because their bone morphological patterns differ from those of humans. Rats show a modeling pattern from youth to old age, while humans show a modeling pattern in the growth stage which changes to a remodeling pattern after maturity [7]. The length and weight of long bones in rats continue to increase up to the old age due to the fact that both or either of the epiphysial growth plates do not close for a long time [8]. The cortical and trabecular bone components of the femur neck in rats have been found to be higher and lower, respectively, than those in humans [3].The age of peak bone mass, hereafter referred to as bone mineral density, in a human is considered a significant indicator for knowing the state of activity of bone metabolism and age of onset of osteoporosis, because thereafter the bone mass reduces with increasing age. To clarify whether rats show peak bone mass or not is an important matter for considering their worth as a model animal in bone researches and for determining corresponding ages between rats and humans in experimental procedures and assessing experimental results. That is, we are interested to know if rats have age-related changes in bone metabolism throughout their life similar to those in humans. We demonstrated that high values of mineral and organic contents at various skeletal sites are present in rats [11] and a peak of bone volume i...

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“…This is because the rate of increase was within 10 percent of the preorchidectomy rate and because all the dogs used in this study were kept in cages and had restricted exercise during the experimental period. Indeed, Wronski et al [21] have shown that body weight did not influence the decrease in bone volume in ovariectomized rats. Although our findings have demonstrated that the loss of sex hormones produced by orchidectomy in male dogs successfully changed both the serum biochemical and histomorphometric parameters, which suggests the occurrence of osteoporosis, the histomorphometric and serum biochemical parameters did not always change in ovariectomized dogs [14,18].…”

Section: Discussionmentioning

confidence: 97%

Effects of Orchidectomy on Bone Metabolism in Beagle Dogs.

Fukuda¹,

Iida²

2000

The Journal of Veterinary Medical Science

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ABSTRACT. The effects of orchidectomy on bone metabolism in male beagle dogs were examined using twelve 2-year-old dogs that were orchidectomized. The dogs' bilateral iliac bones, double-labeled with tetracycline and calcein for the histomorphometry, were obtained from three dogs prior to orchidectomy and at 3, 6, 9, and 12 months afterwards. The serum biochemical constituents related to bone metabolism were examined before and every month after orchidectomy. Between 1 and 6 months after orchidectomy, the value of serum testosterone decreased (1 month), while the levels of parathyroid hormone, calcitonin, total calcium, osteocalcin, and alkaline phosphatase activity increased significantly, indicating a high bone turnover. The mean trabecular thickness and the fraction of labeled osteoid surface decreased significantly 3 months after orchidectomy, but other histomorphometric parameters were unchanged. In the period 7-12 months after orchidectomy, the parathyroid hormone level increased ever and above that of the first 6-month period, while the levels of calcitonin, osteocalcin, alkaline phosphatase activity, and phosphorus decreased. The bone volume, mean trabecular thickness, and the fraction of labeled trabecular surface decreased significantly compared with the pre-orchidectomy values. These findings indicate an imbalance in bone metabolism ( i.e. bone resorption > bone formation). These results indicate that a loss of bone volume accompanied the fall in sex hormone levels following orchidectomy and suggest that the orchidectomized dog is available as an animal model for studying osteoporosis caused by hypogonadism and the decline of sex functions in men.-KEY WORDS: bone histomorphometry, canine, iliac bone, orchidectomy.

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Effect of body weight on osteopenia in ovariectomized rats

Cited by 236 publications

References 15 publications

Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period

Effect of daidzein, 4-methylbenzylidene camphor or estrogen on gastrocnemius muscle of osteoporotic rats undergoing tibia healing period

Age-Related Changes in Bone Mineral Density, Cross-Sectional Area and Strength at Different Skeletal Sites in Male Rats.

Effects of Orchidectomy on Bone Metabolism in Beagle Dogs.

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