Effect of Body Weight on Metabolic Hormones and Fatty Acid Metabolism in Follicular Fluid of Women Undergoing In Vitro Fertilization: A Pilot Study

Nemer, Laurice Bou; Shi, Haolin; Carr, Bruce R.; Word, R. Ann; Bükülmez, Orhan

doi:10.1177/1933719118776787

Cited by 24 publications

(34 citation statements)

References 32 publications

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“…In both clinical studies and animal models, elevated uric acid has been associated with obesity, insulin resistance, abnormal glucose metabolism, and cardiovascular disease (17,33,69). Uric acid has previously been identified in FF and was positively correlated with BMI and associated with cumulus-oocyte degradation in water buffalo (5,9,17). Taken together, these results suggest that ovarian follicles from OW women have increased oxidative stress and reduced antioxidant capacity compared with those from NW women.…”

Section: Discussionmentioning

confidence: 58%

“…As FF content reflects the follicle's metabolism as well as molecules and metabolites needed for the bidirectional somatic cell-oocyte signaling (13,61), our findings suggest that obesity is influencing the microenvironment that controls folliculogenesis, meiotic maturation, and spindle formation in the developing oocyte (46). Other studies have utilized metabolomics to investigate fertility success and pregnancy outcomes (40, 61), but only a few have investigated the effect of obesity on the FF metabolome (5,52,71).…”

Section: Discussionmentioning

confidence: 84%

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Obesity leads to distinct metabolomic signatures in follicular fluid of women undergoing in vitro fertilization

Ruebel

Piccolo

Mercer

et al. 2019

American Journal of Physiology-Endocrinology and Metabolism

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Although obesity negatively influences the metabolic homeostasis of cells within a broad range of tissues, its impact on oocyte metabolism is not fully understood. Prior evidence suggests that obesity increases expression of oocyte genes associated with inflammation, oxidative stress, and lipid metabolism; however, the metabolic impact of these genetic differences is not known. To address this gap, we conducted an exploratory assessment of the follicular fluid (FF) metabolome in eight overweight/obese (OW) and nine normal-weight (NW) women undergoing in vitro fertilization. FF and serum were collected and analyzed by untargeted metabolomics using gas chromatography-quadrupole time-of-flight mass spectrometry and charged-surface hybrid column-electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Untargeted metabolomics identified obesity-associated changes in FF metabolites related to oxidative stress/antioxidant capacity, xenometabolism/amino acid biosynthesis, and lipid metabolism. Discriminant FF metabolites included elevated uric acid, isothreonic acid, one unknown primary metabolite, and six unknown complex lipids in OW compared with NW women. Conversely, 2-ketoglucose dimethylacetal, aminomalonate, two unknown primary metabolites, and two unknown complex lipids were decreased in FF of OW relative to NW women. Indole-3-propionic acid (IPA), a bacteria-derived metabolite, was also decreased in both FF and serum of OW women ( P < 0.05). The significant correlation between antioxidant IPA in serum and FF ( R = 0.95, P < 0.0001) suggests a potential serum biomarker of FF antioxidant status or reflection of the gut metabolism interaction with the follicle. These results suggest that obesity has important consequences for the follicular environment during the preconception period, a window of time that may be important for lifestyle interventions to ameliorate obesity-associated risk factors.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 84%

Obesity leads to distinct metabolomic signatures in follicular fluid of women undergoing in vitro fertilization

Ruebel

Piccolo

Mercer

et al. 2019

American Journal of Physiology-Endocrinology and Metabolism

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“…The clinical features of PCOS often begin during puberty, suggesting that the metabolic and reproductive changes associated with puberty may create fertile ground for triggering the pathophysiology of PCOS [26,59]. IR has a pivotal role in the genesis of the syndrome; it is often seen in patients affected by PCOS and it is exacerbated by obesity [9,60].…”

Section: Polycystic Ovary Syndrome and Obesitymentioning

confidence: 99%

Polycystic Ovary Syndrome in Insulin-Resistant Adolescents with Obesity: The Role of Nutrition Therapy and Food Supplements as a Strategy to Protect Fertility

et al. 2021

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Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in young reproductive-aged women. PCOS is often associated with obesity and impairs reproductive health. Even though several theories have been proposed to explain the pathogenic mechanism of PCOS, the role of insulin resistance (IR) as a key etiological component, independently of (but amplified by) obesity, is well recognized. The consequent hyperinsulinemia activates excessive ovarian androgen production, leading to PCOS. Additionally, the state of chronic inflammation related to obesity impacts ovarian physiology due to insulin sensitivity impairment. The first-line treatment for adolescents with obesity and PCOS includes lifestyle changes; personalized dietary interventions; and, when needed, weight loss. Medical nutrition therapy (MNT) and the use of specific food supplements in these patients aim at improving symptoms and signs, including insulin resistance and metabolic and reproductive functions. The purpose of this narrative review is to present and discuss PCOS in adolescents with obesity, its relationship with IR and the role of MNT and food supplements in treatment. Appropriate early dietary intervention for the management of adolescents with obesity and PCOS should be considered as the recommended approach to restore ovulation and to protect fertility.

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“…The incidence rate of PCOS in obese people is much higher than that in people with normal weight (4). In patients with PCOS, obesity aggravates their reproductive dysfunction, further affects their pregnancy outcomes, and improves their risk of reproductive system tumors and cardiovascular diseases (5). At present, no breakthrough exists in the treatment of obesity (6).…”

Section: Introductionmentioning

confidence: 99%

Correlation Between Fecal Metabolomics and Gut Microbiota in Obesity and Polycystic Ovary Syndrome

Zhou

et al. 2020

Front. Endocrinol.

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This study aimed to explore the relationship between the fecal metabolites and gut microbiota in obese patients with PCOS and provide a new strategy to elucidate the pathological mechanism of obesity and PCOS. Methods: The fecal samples of obese patients with PCOS (n = 18) and obese women without PCOS (n = 15) were analyzed by 16S rRNA gene sequencing and untargeted metabolomics. The peripheral venous blood of all subjects was collected to detect serum sex hormones. The association among fecal metabolites, gut microbiota, and serum sex hormones was analyzed with the R language. Results: A total of 122 named differential fecal metabolites and 18 enrichment KEGG pathways were obtained between the groups. Seven fecal metabolites can be used as characteristic metabolites, including DHEA sulfate. The richness and diversity of gut microbiota in the obese PCOS group were lower than those in the control group. Lachnoclostridium, Fusobacterium, Coprococcus_2, and Tyzzerela 4 were the characteristic genera of the obese patients with PCOS. Serum T level significantly and positively correlated with the abundance of fecal DHEA sulfate (p < 0.05), and serum DHEAS level significantly and negatively correlated with the abundance of fecal teasterone (p < 0.05). Conclusion: Specific fecal metabolites may be used as characteristic metabolites for obese patients with PCOS. The closely relationship among gut microbiota, fecal metabolites, and serum sex hormones may play a role in the related changes caused by hyperandrogenemia.

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Effect of Body Weight on Metabolic Hormones and Fatty Acid Metabolism in Follicular Fluid of Women Undergoing In Vitro Fertilization: A Pilot Study

Cited by 24 publications

References 32 publications

Obesity leads to distinct metabolomic signatures in follicular fluid of women undergoing in vitro fertilization

Obesity leads to distinct metabolomic signatures in follicular fluid of women undergoing in vitro fertilization

Polycystic Ovary Syndrome in Insulin-Resistant Adolescents with Obesity: The Role of Nutrition Therapy and Food Supplements as a Strategy to Protect Fertility

Correlation Between Fecal Metabolomics and Gut Microbiota in Obesity and Polycystic Ovary Syndrome

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