Effect of bone‐like hydroxyapatite/poly amino acid loaded with rifapentine microspheres on bone and joint tuberculosis in vitro

Abstract

Rifapentine-loaded poly(lactic-co-glycolic acid) microspheres (RPMs)-loaded bone-like hydroxyapatite/poly amino acid (BHA/PAA) is effective in curing Staphylococcus aureus-induced chronic osteomyelitis. This study continues to investigate the effect of RPM-loaded BHA/PAA on the bacterial growth of Mycobacterium tuberculosis (MTB), cell proliferation and differentiation in MTB H37Rv-infected MG63 cells. Furthermore, whether Wnt/β-catenin signaling pathway was activated by RPM-loaded BHA/PAA was explored. We found the bactec growth index of H37Rv was significantly inhibited by RPM-loaded BHA/PAA. The MTT assay showed that RPM-loaded BHA/PAA could promote the cell proliferation of H37Rv-infected MG63 cells, as determined by MTT assay. The alkaline phosphatase (ALP) activity and the expression of runt-related transcription factor 2 (Runx2) and osteocalcin (OCN) was examined by commercial kit and Western blot analysis to determine the effect of RPM-loaded BHA/PAA on MTB H37Rv-infected MG63 cell differentiation. It was revealed that RPM-loaded BHA/PAA could promote cell differentiation of H37Rv-infected MG63 cells. Furthermore, we found the expression of Wnt1, LDL receptor related protein 6 (Lrp6) and β-catenin was significantly increased in H37Rv-infected MG63 cells following treatment with RPM-loaded BHA/PAA, as determined by Western blot analysis. In conclusion, this study demonstrated that RPM-loaded BHA/PAA has an effective activity against MTB. RPM-loaded BHA/PAA promoted cell proliferation and cell differentiation of H37Rv-infected MG63 cells. Wnt/β-catenin signaling could be activated by RPM-loaded BHA/PAA in MG63 cells infected with H37Rv. This study demonstrated the potential value of RPM-loaded BHA/PAA in treating bone and joint TB, and suggested Wnt/β-catenin signaling may be an important pathway underlying its function.

Recent Advances in Nanotechnology-Based Strategies for Bone Tuberculosis Management

“…By embedding drugs directly into the scaffold, localized drug delivery can be achieved at the site of the bone TB infection. This method can provide a sustained release of medication, maintaining therapeutic drug levels directly at the infection site, which is crucial for effectively treating TB given the slow replication rate of Mycobacterium TB and the typically prolonged treatment regimens [ 74 , 84 , 141 , 142 ]. Furthermore, the customization of HA scaffolds allows for the tailoring of pore size and porosity to match the specific requirements of the bone defect and the desired drug release profile.…”

Preparation and in vitro osteogenic evaluation of high-strength ceramic artificial bone loaded with anti-tuberculosis drug PaMZ

“…In previous research, Liu et al and Liang et al utilized rifapentine and rifampicin to develop anti-tuberculosis artificial bones. 36,37 As it is known, the use of single or dual anti-tuberculosis drugs has often been associated with the emergence of drug resistance in tuberculosis. In contrast, the triple anti-tuberculosis drug combination of PaMZ employed in this study can effectively reduce the occurrence of tuberculosis drug resistance and expedite the healing of lesions.…”

In vivo therapeutic effect of wollastonite and hydroxyapatite on bone defect

“…The possible mechanism is that the scaffold could provide support for the early formation of microvascular and host bone cell adhesion at the bone defect. Under the stimulation of wollastonite, the bone cells are active and form an osteogenic region around it, thereby exerting the role of bone conduction [41].…”