Effect of Bone Powder/Mesenchymal Stem Cell/BMP2/Fibrin Glue on Osteogenesis in a Mastoid Obliteration Model

Abstract

Background/Aim: This study aimed to prospectively compare the osteogenesis of bone powder (BP) substances with and without mesenchymal stem cells (MSCs) and evaluate the synergistic effect of topically applied recombinant human bone morphogenic protein-2 (BMP2) on MSC-loaded BP using fibrin glue in a mastoid obliteration model. Materials and Methods: To determine the expression of osteocyte-specific genes, total RNA was isolated from three MSC groups: Untreated MSCs, MSCs cultured with BP, and MSCs cultured with BP and BMP2. Real-time polymerase chain reaction was carried out with specific primers of osteogenesis-related genes runt-related transcription factor 2, osteocalcin, osteoprotegerin, osterix, alkaline phosphatase, transforming growth factor beta, and type I collagen. Live/dead staining was also performed. To observe the adhesion of MSCs to the BP, MSCs were treated with BP for 2 days and the surface was observed by scanning electron microscopy (SEM). Under general anesthesia, mastoid obliteration was performed in rats using three groups: treated with BP alone, BP/MSCs, and BP/MSC/BMP2. Before decapitation at 8 weeks post operation, in vivo micro computed tomography (micro CT) was performed. The bullae were dissected, fixed, and decalcified. followed by dehydration, paraffin embedding, and staining by hematoxylin and eosin and Masson's trichrome. Results: SEM

Synergistic Effect of QNZ, an Inhibitor of NF-κB Signaling, and Bone Morphogenetic Protein 2 on Osteogenic Differentiation in Mesenchymal Stem Cells through Fibroblast-Induced Yes-Associated Protein Activation

“…The differentiation of MSCs into osteoblasts plays a key role in bone repair after injuries [34]. Thus, some studies have tried to engraft MSCs with BMP2containing biomaterials to treat bone fractures [35][36][37]. However, the microenvironment of bone defects is complex and contains various types of cells, such as MSCs, fibroblasts and inflammatory cells.…”

Mastoid obliteration and external auditory canal reconstruction using 3D printed bioactive glass S53P4 /polycaprolactone scaffold loaded with bone morphogenetic protein-2: A simulation clinical study in rabbits

“…In a previous study, Jang et al. [ [23] , [24] , [25] ] did a lot of research on introducing 3D printing technology into the field of mastoid obliteration. Composite scaffolds, such as PCL/alginate scaffolds and BP/MSCs/BMP2 scaffolds, have been fabricated and loaded with bioactive factors, such as rhBMP2 and umbilical cord serum, respectively, in an attempt to improve the biological properties of stents.…”

Highly elastic 3D-printed gelatin/HA/placental-extract scaffolds for bone tissue engineering

“…To evaluate new bone formation, ex vivo micro-CT was performed using a Quantum GX micro-CT imaging system (PerkinElmer, Hopkinton, MA, USA) at the Korea Basic Science Institute (Gwangju, Korea). The setting conditions were similar to those used in our previous report 71 . Briefly, the condition was set at 90 kV/80 µA with a field of view of 45 mm (voxel size, 90 µm; scan mode, high resolution; scan time, 4 min), and an approximate dose (X-ray) of 162 mGy radiation per scan.…”