Effect of boron on mycelial growth, sporangiogenesis and zoosporogenesis of Phytophthora nicotianae and the possible inhibitory mechanisms

Qiao, Yu; Shui-ying, Yang; Wang, Han; Li, Zhenlun

doi:10.1007/s10658-017-1244-3

Cited by 5 publications

(4 citation statements)

References 29 publications

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“…According to reported method 62 , the inhibition effect of lactic acid on the sporangia of P. nicotianae was determined. A raw lactic acid solution was mixed with 0.1% KNO 3 to prepare the induced sporulation solution containing different lactic acid concentrations.…”

Section: Methodsmentioning

confidence: 99%

Lactic acid induced defense responses in tobacco against Phytophthora nicotianae

Yan,

Ma,

Peng

et al. 2024

Sci Rep

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Induced resistance is considered an eco-friendly disease control strategy, which can enhance plant disease resistance by inducing the plant’s immune system to activate the defense response. In recent years, studies have shown that lactic acid can play a role in plant defense against biological stress; however, whether lactic acid can improve tobacco resistance to Phytophthora nicotianae, and its molecular mechanism remains unclear. In our study, the mycelial growth and sporangium production of P. nicotianae were inhibited by lactic acid in vitro in a dose-dependent manner. Application of lactic acid could reduce the disease index, and the contents of total phenol, salicylic acid (SA), jasmonic acid (JA), lignin and H2O2, catalase (CAT) and phenylalanine ammonia–lyase (PAL) activities were significantly increased. To explore this lactic acid-induced protective mechanism for tobacco disease resistance, RNA-Seq analysis was used. Lactic acid enhances tobacco disease resistance by activating Ca2+, reactive oxygen species (ROS) signal transduction, regulating antioxidant enzymes, SA, JA, abscisic acid (ABA) and indole-3-acetic acid (IAA) signaling pathways, and up-regulating flavonoid biosynthesis-related genes. This study demonstrated that lactic acid might play a role in inducing resistance to tobacco black shank disease; the mechanism by which lactic acid induces disease resistance includes direct antifungal activity and inducing the host to produce direct and primed defenses. In conclusion, this study provided a theoretical basis for lactic acid-induced resistance and a new perspective for preventing and treating tobacco black shank disease.

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Section: Methodsmentioning

confidence: 99%

Lactic acid induced defense responses in tobacco against Phytophthora nicotianae

Yan,

Ma,

Peng

et al. 2024

Sci Rep

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“…Experimental materials and growth conditions of P. nicotianae Phytophthora nicotianae was isolated from soils where tobacco black shank disease occurred as described previously (Qiao et al, 2017). Isolates were cultured in test tubes with oat medium and were stored at 15 C. Pure isolates were later grown on oat medium for routine experiments.…”

Section: Methodsmentioning

confidence: 99%

“…He et al (2005) have reported that csn2 mutation led to circadian rhythms of conidia becoming abnormal. Our previous research (Qiao et al, 2017) has indicated that the csn4 gene is associated with the inhibitory effect of boron on sporangiogenesis of P. nicotianae. Except for the cited reports, there are few studies of CSN on the regulation mechanism of oomycete development.…”

Section: Introductionmentioning

confidence: 99%

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Luo

Yao

Tan

et al. 2020

PeerJ

Self Cite

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Background Phytophthora nicotianae is a fungal soil-borne pathogen that damages various plant species. Mancozeb and Zineb, fungicides containing manganese (Mn) and zinc (Zn) as the main components, are widely used to control the diseases caused by Phytophthora. However, the inhibition mechanism is still unclear. The purpose of this study was to examine the effects of Mn and Zn on P. nicotianae and to determine possible inhibitory mechanisms of Mn and Zn on sporangiogenesis of P. nicotianae. Methods The mycelial growth, sporangium generation, zoosporogenesis and zoospore germination of P. nicotianae were observed under Mn and Zn treatments. The gene (csn4 and csn7) expression levels of P. nicotianae in different growth stages were examined. Csn4 and csn7 gene expression, superoxide dismutase (SOD) activity, catalase (CAT) activity and malondialdehyde (MDA) content were tested at the stage of sporangiogenesis under different Mn and Zn concentrations. Results Mycelial growth of P. nicotianae was significantly inhibited by Mn from ≥1 mg/L concentration and by Zn from ≥10 mg/L. The sporangia production, sporangia release, and zoospore germination of P. nicotianae were significantly reduced by Mn at all concentrations, while treatment with Zn from ≥0.5 mg/L concentration significantly inhibited the same processes. At the same concentration, the inhibition rate of Mn on the growth process of P. nicotianae was higher than that of Zn. The csn4 and csn7 gene transcription of P. nicotianae were significantly reduced by all treatments with Mn and Zn at the stage of sporangiogenesis. With the increase of Mn concentration, the activities of SOD and CAT increased to maxima and then decreased, and the content of MDA gradually increased during sporangiogenesis of P. nicotianae. The sporangia production of P. nicotianae was significantly positively correlated with the expression levels of the genes csn4 and csn7. Conclusion The inhibitory effect of Mn on the growth process of P. nicotianae was stronger than that of Zn, especially on sporangiogenesis and zoosporogenesis. A possible mechanism of the inhibitory effect on sporangiogenesis of P. nicotianae was that Mn and Zn acted by inhibiting the expression levels of the genes csn4 and csn7 and by affecting antioxidant enzyme activity (further resulting in lipid peroxidation) in the sporangium of P. nicotianae.

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“…The sporangium and zoospores produced on diseased plants can also lead to secondary infection by rain or irrigation water [11]. Under natural conditions, viable zoospores are released from the sporangium, and disease occurrence is positively correlated with the sporangium number [12].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity and Mechanisms of Ethanol Extracts from Scutellaria baicalensis Georgi and Magnolia officinalis against Phytophthora nicotianae

Gao¹,

Shen²,

Xu³

et al. 2023

Phyton

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Phytophthora nicotianae causes substantial economic losses in most countries where tobacco is produced. At present, the control of P. nicotianae mainly depends on chemical methods, with considerable environmental and health issues. We investigated the effects of ethanol extracts from Scutellaria baicalensis Georgi (SBG) and Magnolia officinalis (MO). On mycelial growth, sporangium formation, and zoospore release of P. nicotianae. Both extracts inhibited the growth of P. nicotianae, with mycelial growth inhibition rates of 88.92% and 93.92%, respectively, at 40 mg/mL, and EC 50 values of 5.39 and 5.74 mg/mL, respectively. The underlying mechanisms were the inhibition of sporangium formation, the reduction of zoospore number, and the destruction of the mycelium structure. At an SBG extract concentration of 16.17 mg/mL, the inhibition rates for sporangia and zoospores were 98.66% and 99.39%, respectively. At an MO extract concentration of 2.87 mg/mL, the production of sporangia and zoospores was completely inhibited. The hyphae treated with the two plant extracts showed different degrees of deformation and damage. Hyphae treated with SBG extract showed adhesion and local swelling, whereas treatment with MO extract resulted in broken hyphae. Mixture of the extracts resulted in a good synergistic effect.

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Effect of boron on mycelial growth, sporangiogenesis and zoosporogenesis of Phytophthora nicotianae and the possible inhibitory mechanisms

Cited by 5 publications

References 29 publications

Lactic acid induced defense responses in tobacco against Phytophthora nicotianae

Lactic acid induced defense responses in tobacco against Phytophthora nicotianae

Effects of manganese and zinc on the growth process of Phytophthora nicotianae and the possible inhibitory mechanisms

Antibacterial Activity and Mechanisms of Ethanol Extracts from Scutellaria baicalensis Georgi and Magnolia officinalis against Phytophthora nicotianae

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