Effect of bovine lactoferricin on the growth performance, digestive capacity, immune responses and disease resistance in Pacific white shrimp, Penaeus vannamei

Yi, Zichuan; Huang, He; Li, Xueliang; Wang, Nu-An; Zhong, Guofang

doi:10.1016/j.fsi.2022.03.012

Cited by 16 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The findings suggest a dietary LF intervention could promote the intestinal antioxidant capacity and prevent the intestinal lipid peroxidation in juvenile orange-spotted groupers with SBMIE, through influencing both enzymatic and non-enzymatic antioxidants. Consistent with our results, a reduction in the liver MDA content on day 15 and an increase in the liver T-AOC on day 30 were observed after Aeromonas veronii -induced Nile tilapias were administrated with LF at 0.8 g/kg diet [ 96 ]; increased SOD activity and decreased MDA content in the hepatopancreas were observed in shrimp receiving the diets with 1.5–2.5 g/kg LF [ 97 ]; and similar effects occurred for weaned piglets when they were fed diets with LF [ 37 ]. The above results validated that a dietary LF intervention has a protective effect against oxidative stress resulting from different sources of stress.…”

Section: Discussionsupporting

confidence: 85%

Dietary Lactoferrin Supplementation Improves Growth Performance and Intestinal Health of Juvenile Orange-Spotted Groupers (Epinephelus coioides)

Song

Qin

et al. 2022

Metabolites

View full text Add to dashboard Cite

A 56-day feeding trial was conducted to investigate the effects of dietary lactoferrin (LF) supplementation on the growth performance and intestinal health of juvenile orange-spotted groupers fed high-soybean-meal (SBM) diets. The control diet (FM) and high-soybean-meal diet (SBM60) were prepared to contain 480 g/kg protein and 110 g/kg fat. Three inclusion levels of 2, 6, and 10 g/kg LF were added into the SBM60 to prepare three diets (recorded as LF2, LF6, and LF10, respectively). The results showed that the supplementation of LF in SBM60 increased the growth rate in a dose-dependent manner. However, the feed utilization, hepatosomatic index, whole-body proximate composition, and the abundance and diversity of intestinal microbiota did not vary across the dietary treatments (p > 0.05). After the dietary intervention with LF, the contents of the intestinal malondialdehyde, endotoxin, and d-lactic acid, as well as the plasma low-density lipoprotein cholesterol, high-density lipoprotein cholesterol, and total cholesterol were lower, and the intestinal activities of the glutathione peroxidase, lipase, trypsin, and protease were higher in the LF2-LF10 groups than that in the SBM60 group (p < 0.05). The supplementation of LF in SBM60 increased the muscle layer thickness of the middle and distal intestine and the mucosal fold length of the middle intestine vs. the SBM60 diet (p < 0.05). Furthermore, the supplementation of LF in SBM60 resulted in an up-regulation of the mRNA levels for the IL-10 and TGF-β1 genes and a down-regulation of the mRNA levels of the IL-1β, IL-12, IL-8, and TNF-α genes vs. the SBM60 diet (p < 0.05). The above results showed that a dietary LF intervention improves the growth and alleviates soybean meal-induced enteritis in juvenile orange-spotted groupers. The dietary appropriate level of LF was at 5.8 g/kg, through the regression analysis of the percent weight gain against the dietary LF inclusion levels.

show abstract

Section: Discussionsupporting

confidence: 85%

Dietary Lactoferrin Supplementation Improves Growth Performance and Intestinal Health of Juvenile Orange-Spotted Groupers (Epinephelus coioides)

Song

Qin

et al. 2022

Metabolites

View full text Add to dashboard Cite

show abstract

“…Specifically, bLF has previously demonstrated inhibitory effects on the growth of various Vibrio species, including human pathogenic (non-AHPND) V. parahaemolyticus [30], V. cholerae, V. fluvialis, V. vulnificus and V. alginolyticus [31]. Additionally, preliminary tests suggest that bLF can positively impact fish and shrimp health, with benefits such as an enhanced growth, reduced mortalities, decreased disease outbreaks and stimulated immune responses [17,32,33], making them a potential antibiotic alternative for use in aquaculture. As a crucial first step, this study investigated the in vitro effects of bLF and OT on AHPND-causing V. parahaemolyticus strains from different geographical regions.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, treatment of V. cholerae with bLF resulted in severe membrane damage, such as the occurrence of bacterial protrusion and filamentation [31]. Recently, multiple advantages of using TFs have been proposed for fish [17] and crustaceans [32,33]. Studies with TFs or TF derivatives in shrimp are scarce but rendered positive results such as enhanced growth, reduced mortalities, decreased disease outbreaks and stimulated immune responses [32,33].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, multiple advantages of using TFs have been proposed for fish [17] and crustaceans [32,33]. Studies with TFs or TF derivatives in shrimp are scarce but rendered positive results such as enhanced growth, reduced mortalities, decreased disease outbreaks and stimulated immune responses [32,33]. In this research, we explored the potential of bLF and OT for use in aquaculture against Vibrio parahaemolyticus induced AHPND.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bovine Lactoferrin and Hen Ovotransferrin Affect Virulence Factors of Acute Hepatopancreatic Necrosis Disease (AHPND)-Inducing Vibrio parahaemolyticus Strains

Vandeputte,

Verhaeghe,

Willocx

et al. 2023

Microorganisms

View full text Add to dashboard Cite

Acute Hepatopancreatic Necrosis Disease (AHPND), a highly destructive shrimp disease, has inflicted severe setbacks on the shrimp farming industry worldwide. As the use of antibiotics is discouraged due to emerging antibiotic-resistant bacteria and the pollution of ecosystems, there is a pressing demand for novel, sustainable alternatives. Hence, the influence of bovine lactoferrin (bLF) and hen ovotransferrin (OT), two natural antimicrobial proteins, on the growth of three AHPND-causing Vibrio parahaemolyticus (Vp) strains (M0904, TW01 and PV1) was examined. Additionally, we explored their potential to affect selected Vp virulence factors such as biofilm formation, swimming and swarming, cell surface hydrophobicity, and activity of released lipases and caseinases. Lag phases of all bacterial growth curves were significantly prolonged in the presence of bLF or OT (1, 5 and 10 mg/mL), and bLF (5 and 10 mg/mL) completely inhibited growth of all strains. In addition, bLF or OT significantly reduced biofilm formation (all tested bLF and OT concentrations for Vp M0904 and Vp PV1), bacterial swimming motility (0.5 mg/mL bLF and OT for Vp M0904 and Vp TW01; 1 mg/mL bLF and OT for all strains), cell surface hydrophobicity (for all strains, all bLF and OT concentrations tested except for 0.125 mg/mL OT for Vp PV1) and lipase activity (1 mg/mL bLF and OT for all strains and 0.5 mg/mL bLF and OT for Vp PV1). These promising in vitro results suggest that bLF and/or OT might be used as novel agents for combating AHPND and warrant further research to elucidate the underlying mechanisms of action to fully unlock their potential for AHPND disease management.

show abstract

Histological, Physiological and Transcriptomic Analysis Reveal the Acute Alkalinity Stress of the Gill and Hepatopancreas of Litopenaeus vannamei

et al. 2023

View full text Add to dashboard Cite

Effect of bovine lactoferricin on the growth performance, digestive capacity, immune responses and disease resistance in Pacific white shrimp, Penaeus vannamei

Cited by 16 publications

References 44 publications

Dietary Lactoferrin Supplementation Improves Growth Performance and Intestinal Health of Juvenile Orange-Spotted Groupers (Epinephelus coioides)

Dietary Lactoferrin Supplementation Improves Growth Performance and Intestinal Health of Juvenile Orange-Spotted Groupers (Epinephelus coioides)

Bovine Lactoferrin and Hen Ovotransferrin Affect Virulence Factors of Acute Hepatopancreatic Necrosis Disease (AHPND)-Inducing Vibrio parahaemolyticus Strains

Histological, Physiological and Transcriptomic Analysis Reveal the Acute Alkalinity Stress of the Gill and Hepatopancreas of Litopenaeus vannamei

Contact Info

Product

Resources

About