Tomato (Solanum lycopersicum L.) is an economically important vegetable crop in the globe and affected by various biotic and abiotic stresses to which the plants were exposed during growth. In this study, three tomato hybrids viz., Arka Abeth, CoTH4 4 and Sivam were evaluated the resistant to pest and diseases including biochemical analysis and yield assessment. The experiment results revealed that highest average yield was recorded in Arka Abeth (712.7 q/ha) followed by CoTH4 4 (650.01 q/ha) and lowest in Sivam (574.58 q/ha). Regarding pest and disease, Pin worm was recorded 21.8 % to 41.6% and leaf miner damage was recorded 8.25 % to 12.80 %, Leaf curl virus disease incidence was 0.40 % to 0.61% and wilt incidence was 7.30% to 13.65%. Among the three tomato cultivars arka abeth was performed higher in the biochemical parameters such as ascorbic acid (38.2 mg/ 100g) and lycopene (3.01 mg/100g).