Effect of bromelain and papain gel on enamel deproteinisation before orthodontic bracket bonding

Abstract

Aim To test the hypothesis that enamel surface deproteinisation with different concentrations of bromelain in association with 10% papain increases the shear bond strength (SBS) of brackets bonded with orthodontic composite and resin modified glass ionomer cement (RMGIC). Materials and methods Orthodontic brackets were attached according to the following protocols to 195 bovine incisors, which were acquired and divided into 13 groups: 1) Transbond XT (TXT) according to the manufacturer’s recommendations; 2) Deproteinisation with 3% bromelain (BD) plus 10% papain and TXT; 3) 6% BD plus 10% Papain and TXT; 4) RMGIC, without enamel deproteinisation and without acid etching; 5) RMGIC, with 3% BD plus 10% papain and without acid etching; 6) RMGIC, with 6% BD plus 10% papain and without acid etching; 7) attachment using RMGIC following etching with polyacrylic acid; 8) 3% BD plus 10% papain, attachment using RMGIC and etching with polyacrylic acid; 9) 6% BD plus 10% papain, and attachment using RMGIC following etching with polyacrylic acid; 10) etching with 37% phosphoric acid and attachment using RMGIC; 11) 3% BD plus 10% papain, etching with 37% phosphoric acid and attachment using RMGIC; 12) 6% BD plus 10% papain, etching with 37% phosphoric acid and attachment using RMGIC; 13) deproteinisation with 2.5% sodium hypochlorite (NaOCl), etching with polyacrylic acid and RMGIC. After bonding, the brackets were removed by a universal mechanical testing machine, which recorded shear bond strength at failure. The material remaining on the tooth was assessed using the adhesive remnant index (ARI). Results Deproteinisation with 3% and 6% bromelain gel plus papain significantly increased the shear bond strength (p < 0.05), when acid etching was performed with phosphoric acid, followed by primer application and attachment using Transbond XT (Group 3) and when attached with RMGIC without etching. Deproteinisation with 6% bromelain gel plus papain significantly increased (p < 0.05) the ARI score only when attachment was performed using RMGIC, without etching (Group 6). Conclusions Deproteinisation with bromelain associated with papain in a gel increased the shear bond strength and is recommended before orthodontic bracket attachment.

Effect of Er:YAG, Co2 lasers, papain, and bromelain enzymes dentin treatment on shear bond strength of composite resin

“…We expected that the combination of bromelain and papain enzyme would enhance the SBS based on the reported study (Phiton et al, 2016). Although the SBS of the enzyme combination was higher than the other enzyme groups, this difference was not significant and it was lower in comparison to phosphoric acid.…”

“…It has been reported that removal of unsupported collagen fiber and deproteinization of dentin surface with bromelain enzyme after acid etching has a positive effect on the SBS (Chauhan et al, 2015 ). In another study, the removal of collagen fiber with bromelain enzyme after acid etching has been evaluated and they found that it has a positive effect on SBS (Kochhar et al, 2018 ). All the mentioned studies showed that adding enzymes to the treatment of dentin surface after acid etching had an acceptable effect.…”

Herausforderungen bei der Versorgung von Patienten* mit einer Molaren-Inzisiven-Hypomineralisation

“…Einige Studien berichten, dass durch Deproteinisierung der hypomineralisierten Schmelzoberflä- chen mit 5,25 % Natriumhypochlorit [34], Papain-und Bromelainhaltigen Präparaten [35] der adhäsive Verbund optimiert werden kann. Der verbesserte Halt wurde auch an Brackets nachgewiesen [36].…”