Effect of Bt genetic engineering on indirect defense in cotton via a tritrophic interaction

Blassioli‐Moraes, Maria Carolina; Laumann, R. A.; Aquino, Michely Ferreira Santos de; Paula, Danielli Parrilha de; Borges, Miguel

doi:10.1007/s11248-010-9399-0

Cited by 19 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our findings are consistent with those of other studies that have identified VOCs emitted by cotton (McCall et al, 1994;Loughrin et al, 1995;Paré and Tumlinson, 1998;Röse et al, 1998;Rodriguez-Saona et al, 2003;Hegde et al, 2011;Moraes et al, 2011). Our chemical analyses revealed that herbivore feeding caused increases in the total emission of volatile compounds, as reported previously (Paré and Tumlinson, 1996;Röse et al, 1996), with the type of feeding damage also affecting production.…”

Section: Discussionsupporting

confidence: 93%

Semiochemicals from Herbivory Induced Cotton Plants Enhance the Foraging Behavior of the Cotton Boll Weevil, Anthonomus grandis

et al. 2012

View full text Add to dashboard Cite

The boll weevil, Anthonomus grandis, has been monitored through deployment of traps baited with aggregation pheromone components. However, field studies have shown that the number of insects caught in these traps is significantly reduced during cotton squaring, suggesting that volatiles produced by plants at this phenological stage may be involved in attraction. Here, we evaluated the chemical profile of volatile organic compounds (VOCs) emitted by undamaged or damaged cotton plants at different phenological stages, under different infestation conditions, and determined the attractiveness of these VOCs to adults of A. grandis. In addition, we investigated whether or not VOCs released by cotton plants enhanced the attractiveness of the aggregation pheromone emitted by male boll weevils. Behavioral responses of A. grandis to VOCs from conspecific-damaged, heterospecific-damaged (Spodoptera frugiperda and Euschistus heros) and undamaged cotton plants, at different phenological stages, were assessed in Y-tube olfactometers. The results showed that volatiles emitted from reproductive cotton plants damaged by conspecifics were attractive to adults boll weevils, whereas volatiles induced by heterospecific herbivores were not as attractive. Additionally, addition of boll weevil-induced volatiles from reproductive cotton plants to aggregation pheromone gave increased attraction, relative to the pheromone alone. The VOC profiles of undamaged and mechanically damaged cotton plants, in both phenological stages, were not different. Chemical analysis showed that cotton plants produced qualitatively similar volatile profiles regardless of damage type, but the quantities produced differed according to the plant's phenological stage and the herbivore species. Notably, vegetative cotton plants released higher amounts of VOCs compared to reproductive plants. At both stages, the highest rate of VOC release was observed in A. grandis-damaged plants. Results show that A. grandis uses conspecific herbivore-induced volatiles in host location, and that homoterpene compounds, such as (E)-4,8-dimethylnona-1,3,7-triene and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene and the monoterpene (E)-ocimene, may be involved in preference for host plants at the reproductive stage.

show abstract

Section: Discussionsupporting

confidence: 93%

Semiochemicals from Herbivory Induced Cotton Plants Enhance the Foraging Behavior of the Cotton Boll Weevil, Anthonomus grandis

et al. 2012

View full text Add to dashboard Cite

show abstract

“…Our findings showed that boll weevils responded positively to VOCs released by cotton plants of the genotypes TB90 and Rubi, and that they preferred the volatiles emitted by herbivory-damaged plants compared to volatiles emitted by undamaged plants. In addition, boll weevil feeding on plants caused an increase in the total amount of VOCs produced, consistent with previous studies on other plants showing that elevated VOCs were released in response to herbivore damage from both specialists and generalists (Rodriguez-Saona et al, 2003;Moraes et al, 2011;Magalhães et al, 2012).…”

Section: Discussionsupporting

confidence: 89%

“…In fact, it has been previously known that cotton varieties can differ in the quantity and sometimes in quality of VOCs emission and these changes may be due to herbivory and plant phenological stage (Loughrin et al., ; Magalhães et al., ). Also differences in cotton VOC emission could be a side effect of plant genetic engineering for insect resistance (e.g., Bt‐cotton) (Moraes et al., ) or conventional plant breeding for agronomic purposes, such as higher yield, what can have a direct impact on host plant location by herbivores such as the boll weevil.…”

Section: Introductionmentioning

confidence: 99%

Interaction ofAnthonomus grandisand cotton genotypes: biological and behavioral responses

Silva

Silva-Torres

Moraes

et al. 2015

Entomologia Exp Applicata

Self Cite

View full text Add to dashboard Cite

The boll weevil, Anthonomus grandisBoheman (Coleoptera: Curculionidae), is a key pest of cotton, Gossypium hirsutumL. (Malvaceae). Knowledge about boll weevil feeding and oviposition behavior and its response to plant volatiles can underpin our understanding of host plant resistance, and contribute to improved monitoring and mass capture of this pest. Boll weevil oviposition preference and immature development in four cotton genotypes (CNPA TB90, TB85, TB15, and BRS Rubi) were investigated in the laboratory and greenhouse. Volatile organic compounds (VOCs) produced by TB90 and Rubi genotypes were obtained from herbivore‐damaged and undamaged control plants at two phenological stages – vegetative (prior to squaring) and reproductive (during squaring) – and four collection times – 24, 48, 72, and 96 h following herbivore damage. The boll weevil exhibited similar feeding and oviposition behavior across the four tested cotton genotypes. The chemical profiles of herbivore‐damaged plants of both genotypes across the two phenological stages were qualitatively similar, but differed in the amount of volatiles produced. Boll weevil response to VOC extracts was studied using a Y‐tube olfactometer. The boll weevil exhibited similar feeding and oviposition behavior at the four tested cotton genotypes, although delayed development and production of smaller adults was found when fed TB85. The chemical profile of herbivore‐damaged plants of both genotypes at the two phenological stages and time periods (24–96 h) was similar qualitatively, with 30 identified compounds, but differed in the amount of volatiles produced. Additionally, boll weevil olfactory response was positive to herbivory‐induced volatiles. The results help to understand the interaction between A. grandis and cotton plants, and why it is difficult to obtain cotton genotypes possessing constitutive resistance to this pest.

show abstract

“…The experimental design should include several field plots in future comparative studies. One prospective subject could be examining whether the foraging behavior of parasitoid wasps are disrupted in Bt cotton plots in terms of plant-emitted VOCs compared to conventional cotton plots [55,56]. It is also interesting to study how the genetic engineering affect the indirect interactions between cotton aphid and other herbivores, which can be mediated by plant and/or generalist predators [64,65].…”

Section: Discussionmentioning

confidence: 99%

Transgenic Bt Cotton Does Not Disrupt the Top-Down Forces Regulating the Cotton Aphid in Central China

et al. 2016

View full text Add to dashboard Cite

Top-down force is referred to arthropod pest management delivered by the organisms from higher trophic levels. In the context of prevalent adoption of transgenic Bt crops that produce insecticidal Cry proteins derived from Bacillus thuringiensis (Bt), it still remains elusive whether the top-down forces are affected by the insect-resistant traits that introduced into the Bt crops. We explored how Bt cotton affect the strength of top-down forces via arthropod natural enemies in regulating a non-target pest species, the cotton aphid Aphis gossypii Glover, using a comparative approach (i.e. Bt cotton vs. conventional cotton) under field conditions. To determine top-down forces, we manipulated predation/parasitism exposure of the aphid to their natural enemies using exclusion cages. We found that the aphid population growth was strongly suppressed by the dominant natural enemies including Coccinellids, spiders and Aphidiines parasitoids. Coccinellids, spiders and the assemblage of other arthropod natural enemies (mainly lacewings and Hemipteran bugs) are similarly abundant in both plots, but with the parasitoid mummies less abundant in Bt cotton plots compared to the conventional cotton plots. However, the lower abundance of parasitoids in Bt cotton plots alone did not translate into differential top-down control on A. gossypii populations compared to conventional ones. Overall, the top-down forces were equally strong in both plots. We conclude that transgenic Bt cotton does not disrupt the top-down forces regulating the cotton aphid in central China.

show abstract

Effect of Bt genetic engineering on indirect defense in cotton via a tritrophic interaction

Cited by 19 publications

References 37 publications

Semiochemicals from Herbivory Induced Cotton Plants Enhance the Foraging Behavior of the Cotton Boll Weevil, Anthonomus grandis

Semiochemicals from Herbivory Induced Cotton Plants Enhance the Foraging Behavior of the Cotton Boll Weevil, Anthonomus grandis

Interaction ofAnthonomus grandisand cotton genotypes: biological and behavioral responses

Transgenic Bt Cotton Does Not Disrupt the Top-Down Forces Regulating the Cotton Aphid in Central China

Contact Info

Product

Resources

About