Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Beran, Jan; Šimoník, Ondřej; Stádník, Luděk; Rajmon, Radko; Ducháček, Jaromír; Krejcárková, A.; Doležalová, Martina; Šichtař, Jiří

doi:10.11118/actaun201361061575

Cited by 11 publications

(10 citation statements)

References 21 publications

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“…In general we can say that considerable individual diff erences in sperm survivability between bulls were determined. This is in accordance with the work of Beran et al (2012 and2013).…”

Section: Discussionsupporting

confidence: 92%

“…The potential fertility of sperm can be evaluated by several methods (Dhurvey et al, 2012). Cold shock test was evaluated in relation to LDL addition to diluters (Beran et al, 2013), but optimal LDL concentration increasing resistance of spermatozoa against cold shock was not suggested. Thus, the aim of this study was to determine the eff ect of the LDL addition to selected diluters on the rate of spermatozoa survival following cold shock and propose the optimal combination of the diluter and LDL concentration.…”

Section: Introductionmentioning

confidence: 99%

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Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock

Beran¹,

Šimoník²,

Rajmon³

et al. 2016

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The aim of work was to determine the effect of LDL cholesterol addition into selected diluters on the resistance of spermatozoa against cold shock and on their short-term survivability during cold test. The hypothesis was that the addition of LDL cholesterol will positively affects sperm resistance to cold shock and ensures a higher survivability of spermatozoa during short-term cold survival test. Four bulls of different breeds and ages, from the same sire insemination center were used. A total of eight semen collections were processed. Each ejaculate was divided into 6 portions (3 controls and 3 samples). Three commercially produced diluters, AndroMed®, Bioxcell®, and Triladyl® were used, each in standard and LDL enriched variants. In the case of AndroMed® or Bioxcell®, 6% of LDL was simply added. In Triladyl®, 10% of LDL replaced the standard egg yolk component. Spermatozoa resistance to cold shock was evaluated by the percentage of live sperm using Eosin-Nigrosine staining. The results showed the influence of bull individuality as an important factor. It is possible to recommend Bioxcell® with addition of LDL cholesterol in 6% concentration, which survivability was 69.17% at the beginning of the test, and 52.94% after 2 hours of incubation.

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“…In general we can say that considerable individual diff erences in sperm survivability between bulls were determined. This is in accordance with the work of Beran et al (2012 and2013).…”

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock

Beran¹,

Šimoník²,

Rajmon³

et al. 2016

Acta Univ. Agric. Silvic. Mendelianae Brun.

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“…The factors of bull's individuality or thawing method were detected as important in presented study as well as in many previous (Beran et al, 2013;Paldusová et al, 2016;Doležalová et al, 2016). Both these criteria should be therefore tested and proven in specified conditions to optimize methods of IDs processing.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Thawing Methods on Bull's Semen Characteristics

Doležalová

Ptáček

Stádník

et al. 2017

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The aim of the study was to evaluate the influence of different thawing methods on bull's semen characteristics. The semen was collected and processed from 8 bulls (Holstein, n = 4; Czech Fleckvieh, n = 4) kept in private Sire insemination Centre. Four different thawing methods were tested: control thawing methods (temperature = 38.5 °C, time = 30 seconds), slow thawing method (temperature = 30 °C, time = 50 seconds), moderate thawing method (temperature = 50 °C, time = 15 seconds), rapid thawing rate (temperature = 70 °C, time = 3 seconds). The percentage rate of total and progressive motile spermatozoa above head as well as movement characteristics of straight-line velocity (VSL, µm/s) and linearity (LIN, %) were recorded using CASA system immediately after thawing and after 2 hrs. of heat incubation (±38 °C). Subsequently the differences between semen characteristics measured immediately after thawing and after 2 hours of incubation were calculated. The data were analyzed with SAS software. All the evaluated semen traits reached significantly lowest values in total motility (−8 % to −10.5 %, P < 0.05), progressive motility (−5.6 % to −10 %, P < 0.05) VSL (−3.9 µm/s to −9 µm/s, P < 0.05) and LIN (−1.5 % to −4.8 %, P < 0.05) in control thawing method immediately after the thawing compared to others. However, these differences were negligible after 2 hrs. of incubation. The highest values of progressive motility and movement characteristics after thawing and after 2 hours incubation were detected using slow thawing method. Moreover, the rapid method of thawing showed the significantly the lowest results in progressive motility (−3.6 % to −6.6 %), VSL (−3.2 to −5.6 µm/s) and LIN (−3 % to −4.6 %) characteristics assessed after 2 hrs. of incubation compared the others (P < 0.05). Control method of thawing was most stable during the incubation and showed the significantly lowest decrease of total motility (−7.6 % to −11.8 %, P < 0.05), progressive spermatozoa motility above head (−7.5 % to −9.8 %, P < 0.05) and VSL characteristics (−2.4 to −9.7 µm/s, P < 0.05) during incubation. Therefore, this thawing protocol can be recommended at this study based on the lowest spermatozoa characteristics decline during incubation for using artificial insemination.

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“…However, resistance of sperm against cold shock has not been evaluated as well in relation to the addition of LDL. Moreover, the results of a previous study (Beran et al 2013b) have shown different results in relation to the concentration of LDL.…”

Section: Reproduction Bull Sperm Extender Ldl Cholesterol Sperm Smentioning

confidence: 95%

Influence of selected factors on bovine spermatozoa cold shock resistance

et al. 2015

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The objectives of this study were to determine the effects of sire, extender, and addition of Low Density Lipoprotein (LDL) to extenders used on the percentage rate of spermatozoa survival after cold shock. Two groups of extenders were compared: without LDL addition (control variants) and LDL enriched (experimental variants). Three extenders were used: AndroMed ® extenders, and 6-10% LDL addition into the Triladyl ® extender. In total, 12 samples of fresh semen were collected from 4 bulls during a period of 8 weeks. Bovine spermatozoa cold shock resistance (1 ± 1 °C, 10 min) was evaluated by the percentage rate of live sperm using eosin-nigrosine staining immediately and after heat incubation (37 ± 1 °C, 120 min). The results showed the effect of sire as important and individual differences between selected sires in their sperm resistance against cold shock were confirmed. AndroMed ® and Bioxcell ® were found to be providing better protection of bull semen to cold shock compared to Triladyl ® due to lower decline of live sperm proportion. Our results detected a positive effect of LDL addition on sperm resistance against cold shock, especially on lower decrease of live sperm percentage rate after 120 min of the heat test (P < 0.05). Further studies are needed to assess the optimal concentration of LDL in various kinds of extenders as well to state ideal time and temperature conditions for ensuring LDL reaction with sperm. , bull sperm, extender, LDL cholesterol, sperm survival, cold shock, eosin-nigrosine staining Reproduction

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Effect of bull, diluter and LDL-cholesterol concentration on spermatozoa resistance against cold shock

Cited by 11 publications

References 21 publications

Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock

Effect of LDL Addition Into Selected Bull Sperm Diluters on Resistance of Spermatozoa Against Cold Shock

Effect of Different Thawing Methods on Bull's Semen Characteristics

Influence of selected factors on bovine spermatozoa cold shock resistance

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