Effect of buscopan, a compound that alleviates cramps, on the developing nervous system of the chick embryo

Ertekin, Ayşe; Atay, Emre; Bozkurt, Erhan; Aslan, Esra

doi:10.1002/bdr2.1929

Cited by 6 publications

(8 citation statements)

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“…The early chick embryo model is an ideal model that mimics the first months of embryonic development in mammals. The chick embryo model used in the study we presented has also been used in many studies to gain preclinical knowledge about the teratogenicity of drugs (10,15,16). We investigated morphologic and histopathologic effects of LCM at different doses on embryonic development in a chick embryo model for possible teratogenic effects of LCM, which considered as category C (17).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of anti-epileptic Lacosamide treatment on neural tube development: Chick embryo experimental model

et al. 2022

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Objective: Even if epileptic women can give birth to healthy babies, the rates of premature birth, low birth weight, fetal and neonatal death risk, congenital malformations and growth retardation in pregnant women who use the antiepileptic drugs (AEDs) are high compared to the population. The present study aimed to examine the effects of AED Lacosamide (LCM) during pregnancy on the fetus in terms of neuronal and embryonic development in 48-hour chick embryo model. Material and Methods: 40SPF fertilized eggs divided into 4 equal numbers. At the 28th hour, LCM was applied in the sub-blastoderm, and the experiment was terminated at the 48th hour. Embryos were evaluated morphologically by neural tube position, somite number, and cranio-caudal length. Histopathologically, hematoxylin-eosin, Caspase-3 immunohistochemistry, and TUNEL staining were performed and and assessed for cell death. Results: LCM adversely affected neural tube position in groups 3 and 4 compared to control. In addition, it regressed embryonic development by decreasing somite number and craniocaudal length in groups 3 and 4. When evaluated in terms of apoptotic cell death, LCM increased caspase-3 immunoreactivity and the number of TUNEL-positive cells in groups 3 and 4, respectively (p=0.002), (p≤0.001). Conclusion: LCM was caused to regression of embryonic development and impaired neural tube position in early chick embryo model, dose-dependent manner. It increased cell death and showed teratogenic effects in the early embryo model. The usage of LCM for pregnant women should be considered carefully. It is obvious that more preclinic studies are needed to demonstrate LCM effects comprehensively.

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Section: Discussionmentioning

confidence: 99%

Evaluation of anti-epileptic Lacosamide treatment on neural tube development: Chick embryo experimental model

et al. 2022

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“…However, changes in AgNOR protein levels also explain the metabolic movements of cells (Akar & Sur, 2010; Derenzini et al, 1995; Gündüz & Oznurlu, 2014; Lindner, 1993; Mamaev & Mamaeva, 1990). AgNOR, which was previously applied as a malignancy parameter, has also been used to evaluate cancer prognosis (Derenzini, Ceccarelli, Santini, Taffurelli, & Treré, 2004; T. Ertekin et al, 2017), the cells of the heart, lung, and femoral muscle (Çolakoğlu et al, 2015; Kandiş et al, 2016; Saritas et al, 2016), toxic effect of the drugs (A. Ertekin et al, 2021; Rakip et al, 2021) and ischemia/reperfusion injury (Nisari et al, 2016) in recent studies.…”

Section: Discussionmentioning

confidence: 99%

“…Ertekin et al, 2017), the cells of the heart, lung, and femoral muscle (Çolako glu et al, 2015;Kandis ȩt al., 2016;Saritas et al, 2016), toxic effect of the drugs (A. Ertekin et al, 2021;Rakip et al, 2021) and ischemia/ reperfusion injury (Nisari et al, 2016) in recent studies.…”

Section: Discussionmentioning

confidence: 99%

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Effect of tramadol hydrochloride on neural tube development in 48‐hr chick embryos: Argyrophilic nucleolar organizing region and genetic analysis study

Atay

Bozkurt

Ertekin

2022

Birth Defects Research

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Background: Tramadol hydrochloride or tramadol is an opioid analgesic that acts on the central nervous system. The pregnancy category of tramadol is determined as "C" according to the Food and Drug Administration. There are no adequate and well-controlled studies in pregnant women. In this study, we aimed to reveal the effects of tramadol on neural tube (midline) closure by analyzing morphologically, histologically and genetically in chick embryos.Methods: Ninety White Leghorn species, fertile and 0-day-old specific pathogen-free eggs (60 ± 5 g) were used in the study. Eggs were divided into a total of six groups (control, sham, and drug groups). Four different doses of tramadol (1, 2.5, 5, and 7.5 mg/egg) were administered subblastodermically at the 28th hour of the incubation. All eggs were opened at the 48th hour of incubation and evaluated. Results: Embryos in the control group according to Hamburger-Hamilton classification were compatible with stages 13 and 14. In the groups treated with tramadol, it was determined that the embryos had neural tube closure defects (such as neck, tail regions) and some embryos showed developmental retardation due to the increase in the drug dose. In the statistical analysis performed, a significant difference was found between the control group and the group receiving the highest dose of tramadol in terms of crown-rump length and number of somites (p < .05). The brain and reproductive expression gene expression was upregulated in embryos at each of tramadol doses compared to control group.Conclusions: It was determined that tramadol causes neural tube closure defects in embryos depending on the dose.

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“…Drugs that are sometimes required to be used during pregnancy cause such congenital malformations, but nowadays, the pregnancy category of newly developed drugs and what kind of malformations they may cause are not fully known due to the lack of studies (Robert, 2000; Rowland et al, 2006; Song et al, 2012; Tureci et al, 2011). However, many drugs that are thought to cause congenital malformations have been questioned in terms of neural tube defects by experimental animal model studies (Atay et al, 2020; Ertekin et al, 2019; Ertekin et al, 2021; Rakip et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Effect of sugammadex administration on neural tube development in 48‐h chick embryos

Büyükerkmen,

Atay,

Firat

et al. 2023

Microscopy Res & Technique

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Sugammadex is a new generation drug that has led to significant changes in the practice of anesthesia. However, its effects on fetal development are not yet fully known. The aim of this study is to investigate the teratogenic effects of sugammadex on neural tube and embryonic development in early chick embryos. In this study, 50 0‐day fertile specific non‐pathogenic (SPF) eggs were used. Fifty eggs were divided into 5 different groups, each consisting of 10 pieces. While no substance was given to the control group at the 28th hour of the study, 4 different doses of sugammadex were administered to the experimental groups, respectively 2, 4, 8, 16 mg/kg. Cranio‐caudal lengths of embryos, somite numbers, average number of argyrophilic nucleolar regulatory regions (AgNOR) per nucleus, total AgNOR area/total nuclear area (TAA/NA) ratios, Caspase‐3 H‐Score results, and presence of neural tube defect were compared among the groups. While the mean cranio‐caudal lengths, somite counts, TAA/NA ratios and AgNOR counts of the embryos were found to be statistically significantly lower than the control group, Caspase‐3 H‐Score mean results were found to be significantly higher (p < .05). In addition, it was observed that there was an increase in neural tube patency and developmental delay. As a result, sugammadex crossing the placenta was revealed to increase the release of proapopitotic molecules and disrupt the developmental stages of embryos. Thus, it was determined that sugammadex in increased developmental delay and incidence of neural tube defects in early chick embryos with increased dose dependent. Despite these results, the effects of sugammadex on fetal development in in vivo and in vitro environments should be studied with further studies.Research Highlights Sugammadex is a new generation drug that has led to significant changes in the practice of anesthesia. However, its effects on fetal development are not yet fully known. It has been observed that different doses of sugammadex increase the risk of neural tube defect development on chick embryos and slow the embryo development in a dose‐dependent manner.

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Effect of buscopan, a compound that alleviates cramps, on the developing nervous system of the chick embryo

Cited by 6 publications

References 79 publications

Evaluation of anti-epileptic Lacosamide treatment on neural tube development: Chick embryo experimental model

Evaluation of anti-epileptic Lacosamide treatment on neural tube development: Chick embryo experimental model

Effect of tramadol hydrochloride on neural tube development in 48‐hr chick embryos: Argyrophilic nucleolar organizing region and genetic analysis study

Effect of sugammadex administration on neural tube development in 48‐h chick embryos

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