Effect of butylated hydroxytoluene on bull spermatozoa frozen in egg yolk-citrate extender

Optimal inclusion level of butylated hydroxytoluene in semen extender improves the quality of post-thawed canine sperm

“…These findings are in accordance with a recent work done, in which BHT at high concentrations of 1.6mM significantly decreased the sperm motility, viability, and acrosomal integrity in canine semen (Sahashi et al, 2011). The damage inflicted on the sperm membrane during cryopreservation renders the membrane more permeable to the BHT metabolites which, in turn, has a harmful effect (Shoae and Zamiri, 2008;Sahashi et al, 2011). Similarly, BHT was found to be toxic for cattle bull (Shoae and Zamiri, 2008), goat (Khalifa et al, 2008), human (Aitken and Clarkson, 1988) and boar (Roca et al, 2004) spermatozoa.…”

“…However, owing to the paucity of literature regarding the use of antioxidants in canine species, the results need to be discussed in comparison to their use in other animals/ species. Similar improvements in semen quality by the use of BHT have been reported for turkey tom (Donoghue and Donoghue, 1997), cattle bull (Chaterjee and Gagnon, 2001;Shoae and Zamiri, 2008), ram (Watson and Anderson, 1983), goat (Khalifa et al, 2008), and boar (Roca et al, 2004) spermatozoa. The protective effect of antioxidant (BHT) is attributed to two mechanisms: firstly, the incorporation of the compound in the sperm membranes, hence, making them more fluidic and preventing them from the damage (Ijaz et al, 2009) and, secondly, declining the damaging potential of lipid peroxyl radicals by conversion into hydroperoxides (Thomassen et al, 2006).…”

“…Sahashi et al (2011) have recently reported an optimal inclusion level of 0.4mM BHT in canine semen. The optimal inclusion level for BHT seems to be a species specific characteristics as indicated by previous works for boar (Roca et al, 2004), cattle bull (Shoae and Zamiri, 2008), and buffalo bull (Ijaz et al, 2009) semen recorded as 0.05−2.0, 0.5−1.0, and 1.0−2.0mM, respectively. Variability in results may be due to variability in concentration of spermatozoa used in respective studies.…”

“…Butylated hydroxytoluene (BHT) is one such example being tested for its cryoprotective potentiality. BHT has been used successfully for preservation of liquid semen in turkey tom (Donoghue and Donoghue, 1997) and to minimize cryoinjury in ram (Watson and Anderson, 1983), boar (Roca et al, 2004), cattle bull (Shoae and Zamiri, 2008), and goat spermatozoa (Khalifa et al, 2008). However, there are scanty reports (Neagu et al, 2010;Sahashi et al, 2011) regarding its cryoprotective potentiality and optimal inclusion level in canine semen.…”

Efeito de diferentes concentrações de melatonina em espermatozoides de carneiros sobre estresse oxidativo após criopreservação

“…A variação do efeito observado com as diferentes concentrações de melatonina pode ser explicada pelo fato de que quantidades excessivas de antioxidante, causam alta fluidez da membrana plasmática, acima do ponto desejado, deixando os espermatozoides mais propensos a danos no acrossoma (Ashrafi et al 2011, Shoae & Zamiri 2008. Diante disso, a concentração de antioxidante adicionado ao diluente deve ser considerada, devido às possíveis alterações na condição fisiológica do diluente seminal que ela pode causar.…”