Effect of C/N ratio on the PHA accumulation capability of microbial mixed culture fed with leachates from the organic fraction of municipal solid waste (OFMSW)

Valencia, Ángel Iván Sánchez; Zamora, Ulises Rojas; Rodríguez, Mónica Meráz; Ramírez, José Álvarez; Peláez, Mónica Liliana Salazar; Fajardo-Ortiz, María del Carmen

doi:10.1016/j.jwpe.2021.101975

Cited by 22 publications

(6 citation statements)

References 40 publications

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“…However, for P. kondratievae PHA accumulation decreased at a C/N ratio higher than 40 for all carbon sources (Figure 4 ). This can be explained by an inhibition effect caused by high concentrations of carbon compounds (Sánchez Valencia et al, 2021 ). It was observed that PHA accumulation decreased by increasing the C/N ratio to a certain extent, which differed from one carbon source to another.…”

Section: Discussionmentioning

confidence: 99%

Paracoccus kondratievae produces poly(3‐hydroxybutyrate) under elevated temperature conditions

Moanis,

Geeraert,

Van den Brande

et al. 2024

Environ Microbiol Rep

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As part of ongoing efforts to discover novel polyhydroxyalkanoate‐producing bacterial species, we embarked on characterizing the thermotolerant species, Paracoccus kondratievae, for biopolymer synthesis. Using traditional chemical and thermal characterization techniques, we found that P. kondratievae accumulates poly(3‐hydroxybutyrate) (PHB), reaching up to 46.8% of the cell's dry weight after a 24‐h incubation at 42°C. Although P. kondratievae is phylogenetically related to the prototypical polyhydroxyalkanoate producer, Paracoccus denitrificans, we observed significant differences in the PHB production dynamics between these two Paracoccus species. Notably, P. kondratievae can grow and produce PHB at elevated temperatures ranging from 42 to 47°C. Furthermore, P. kondratievae reaches its peak PHB content during the early stationary growth phase, specifically after 24 h of growth in a flask culture. This is then followed by a decline in the later stages of the stationary growth phase. The depolymerization observed in this growth phase is facilitated by the abundant presence of the PhaZ depolymerase enzyme associated with PHB granules. We observed the highest PHB levels when the cells were cultivated in a medium with glycerol as the sole carbon source and a carbon‐to‐nitrogen ratio of 10. Finally, we found that PHB production is induced as an osmotic stress response, similar to other polyhydroxyalkanoate‐producing species.

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Section: Discussionmentioning

confidence: 99%

Paracoccus kondratievae produces poly(3‐hydroxybutyrate) under elevated temperature conditions

Moanis,

Geeraert,

Van den Brande

et al. 2024

Environ Microbiol Rep

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“…The nutrient feeding was prepared to have C:N:P = 100:8:1.5, corresponding to the full availability of ammonium and the limiting phosphorus [24]. This choice was made because, from a plant engineering perspective, it will be easier to control phosphorus than to control ammonium [25,26] O (0.002).…”

Section: Selection and Acclimation Stagementioning

confidence: 99%

Influence of Aeration Rate on Uncoupled Fed Mixed Microbial Cultures for Polyhydroxybutyrate Production

Castagnoli,

Falcioni,

Touloupakis

et al. 2024

Sustainability

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The use of residual streams as feedstock for the production of polyhydroxyalkanoates (PHAs) is growing steadily, as it allows the valorization of waste and nutrients otherwise disposed of and the potential production of a biodegradable bioplastic. To date, the environmental and economic costs associated with this process limit its scale-up, which is why it is important to identify possible solutions and optimize the costliest steps. With this in mind, a laboratory-scale sequenced batch reactor (SBR, 5 L) was constructed to allow the selection of a mixed microbial culture able to convert volatile fatty acids (VFAs) into PHA. The reactor is fed with synthetic water containing VFAs, ammonium, phosphate, and micronutrients, typical compounds of fermented streams of certain wastes, such as cheese whey, food waste, or wastewater sludge. The biomass selected and produced by this first reactor is sent to an accumulation reactor, which is fed with a solution rich in VFAs, allowing the accumulation of PHAs. The role of aeration and its impacts on the main process parameters were analyzed. Three scenarios corresponding to different aeration rates were analyzed: 0.08, 0.16, and 0.32 vvm. The SBR was operated at an organic load rate of 600 mgCOD L−1d−1, under a dynamic feeding regime (feast–famine) and a short hydraulic retention time (HRT; 1 day). The results obtained showed that a value of 0.32 enabled better selection and better settling of the sludge. Furthermore, a potential correlation between aeration rate and VFA and NH4+ consumption rates was identified. The resulting biomass was able to accumulate up to 0.15 ± 0.02 g PHAgVSS−1.

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“…After the aeration phase comes the reaction phase, which starts the nitrification and denitrification process, appropriate time exposure to the reaction process, and nitrification leads to a complete transition to nitritation and increased nitrogen removal efficiency (25)(26). The settling phase will show how much the organic removal reaches the peak point by separation by gravity between sedimented biomass and supernatant.…”

Section: Cite This Asmentioning

confidence: 99%

Potential Sequencing Batch Reactor in Leachate Treatment for Organic and Nitrogen Removal Efficiency

Viareco,

Adriansyah,

Sufra

2023

JKL

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Introduction: Landfill wastewater or leachate has unique characteristics. Several conventional methods need a few treatment tanks to cover the treatment process. A Sequencing Batch Reactor (SBR) is a biological treatment method that uses a single tank with few-cycle phases. This study aims to determine the potential of a Sequencing Batch Reactor (SBR) that can be used to treat landfill leachate. Methods: A Sequencing Batch Reactor (SBR) was used in this study. The characteristics of leachate served as the basis for the design of the SBR reactor. The synthetic leachate was used to create the reactor feed based on the actual landfill leachate compositions. The reactor's influent and effluent were analyzed based on a few parameter changes, such as Suspended Solid (SS), Chemical Oxygen Demand (COD), ammonia nitrogen (NH3-N), and Total Kjeldahl Nitrogen (TKN). Results and Discussion: While the experiment was running, 84% of SS were removed, 88% of COD were removed, a -28% efficiency rate for NH3-N removal, and a -172% efficiency rate for TKN removal. After the withdrawal phase, the supernatant showed promising results with 99% COD removal, 97% SS removal, and 65% TKN removal. Unfortunately, the removal of NH3-N reaches -130%. It indicates insufficient concentrations of MLSS and SRT, as well as the presence of a eutrophication process. Conclusion: SBR shows a promising result for leachate treatment. Few changes in the cycling process were needed for further study, so the treatment process becomes optimal to reach its full potential.

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Effect of C/N ratio on the PHA accumulation capability of microbial mixed culture fed with leachates from the organic fraction of municipal solid waste (OFMSW)

Cited by 22 publications

References 40 publications

Paracoccus kondratievae produces poly(3‐hydroxybutyrate) under elevated temperature conditions

Paracoccus kondratievae produces poly(3‐hydroxybutyrate) under elevated temperature conditions

Influence of Aeration Rate on Uncoupled Fed Mixed Microbial Cultures for Polyhydroxybutyrate Production

Potential Sequencing Batch Reactor in Leachate Treatment for Organic and Nitrogen Removal Efficiency

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