Effect of C. Parvum on immunization with irradiated tumour cells

Woodruff, M. F. A.; Ghaffar, Abdul; Dunbar, Noreen; Whitehead, Valerie L.

doi:10.1038/bjc.1976.79

Cited by 16 publications

(3 citation statements)

References 2 publications

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“…Similarly Bomford (1975) has demonstrated suppression of footpad challenge with Mc-induced sarcoma in the mouse, and similar findings are reported by Scott (1975) with a mouse mastocytoma. With a mouse mammary carcinoma and the Shay chloroma in the rat, however, incorporation of C. parvum into intraperitoneal vaccines of irradiated cells did not improve their therapeutic efficiency (Likhite and Halpern, 1973), while the immunotherapeutic effect of irradiated mouse sarcoma cells was abolished by systemic injection of C. parvum or by addition of large doses of the agent (Woodruff et al, 1976a;.…”

Section: Macrophage-depleted Ratsmentioning

confidence: 99%

C. Parvum immunotherapy of transplanted rat tumours

Pimm

Baldwin

1977

Intl Journal of Cancer

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C.parvum (Wellcome CN 6134) has been tested for tumour suppression against a range of syngeneically transplanted rat tumours, both carcinogen-induced and of spontaneous origin. Subcutaneous growth was not prevented by distant subcutaneous or intravenous injection of the preparation, although growth rates were sometimes depressed or accelerated. In contrast, C. parvum injected in admixture with tumour cells consistently suppressed their growth and with highly immunogenic tumours induced systemic tumour immunity, C. parvum injected intravenously retarded development of pulmonary tumour deposits, and intrapleural injection suppressed growth of pleural tumours and malignant effusions. Host immunosuppression failed to abrogate the tumour-suppressive effect of locally applied C. parvum, but host macrophage depletion with silica totally abolished the response. These studies indicate that in the rat, tumour suppression is most consistently achieved by regional application of C. parvum, and that this response is more dependent upon local macrophage stimulation than generation of systemic immune responses.

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Section: Macrophage-depleted Ratsmentioning

confidence: 99%

C. Parvum immunotherapy of transplanted rat tumours

Pimm

Baldwin

1977

Intl Journal of Cancer

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“…Inhibition was not seen when C. parvum was given prior to or on the same day as alloimmunization. All these parameters are known to be impor-tant factors in the ability of C. parvum to modulate other immune functions, such as antibody responses (Warr and Sljivic, 1974) and tumor rejection (Sadler and Castro, 1975;Woodruff et al, 1976). Experiments to answer these questions are in progress.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of cell-mediated cytotoxicity to tumor alloantigens by systemic administration of Corynebacterium parvum

Murahata

Zighelboim

1979

Cancer Immunol Immunother

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Intravenous administration of C. parvum following SC immunization with a tumor allograft markedly impaired the generation and expression of primary cell-mediated cytotoxicity to the immunizing alloantigens. For the inhibition to become manifest, a minimum of 4 days of in vivo exposure to C. parvum was necessary, and the effect was seen only when C. parvum was injected during the developing immune response; injection prior to or simultaneously with alloimmunization resulted in no impairment. The inhibition of cell-mediated cytotoxieity was selective. While spleen eell populations exhibited decreased eytotoxie aetivity, lymphoid eell populations from lymph nodes and blood were unaffected. The inhibitory effeet was restricted to the cellular component of the immune response. Complement-dependent cytotoxic antibodies developed normally and their titer was comparable to that of control alloimmunized mice.reported here indicate that systemic administration of C.parvum during a developing immune response to an SC inoculum of allogeneic tumor cells has a selective effect on the cell-mediated compartment of the immune response. While inhibition of primary cell-mediated cytotoxicity was detectable in the spleen, expression of cytotoxic activity in other lymphoid organs and generation of cytotoxic antibodies were unaffected. Materials and Methods MiceMale C57BL/6 mice (Strong, San Diego, CA) were obtained at 6-8 weeks of age and rested for at least 1 week before the initiation of an experiment. For some experiments, male BALB/c mice (Jackson Laboratory, Bar Harbor, ME) were used. Assay of Cell-Mediated Cytotoxic ActivityMice were sacrificed by cervical dislocation, and a single cell suspension of spleen cells prepared by expression of the organ through a stainless steel mesh. In some experiments, inguinal and mesenteric lymph nodes were pooled and prepared in the same manner. Cytotoxic activity was measured by a chromium release assay, the immunizing cell being used as the target cell, as described by Brunner et al.

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“…The dose, route, and frequency of administration of these stimulators has been determined both prophylactically and therapeutically in order to establish what regimen of treatment is needed to produce maximal suppression of tumor growth (Woodruff et al, 1976;Scott and Bomford, 1976;Smith and Scott, 1972;Halpern et al, 1973;Mohr et al, 1975;Pearson et al, 1973;DiLuzio, 1975;Chirigos et al, 1973;Milas, 1973). However, relatively little work has appeared in the literature concerning the therapeutic application of immunopotentiators in animal systems expressing tumors of hematopoietic origin (i.e., leukemias) (Math~ et al, 1973a(Math~ et al, , b, 1974Likhite and Halpern, 1973;Pearson et al, 1972).…”

Section: Introductionmentioning

confidence: 99%