Effect of cadmium and zinc on attachment and detachment interactions of Pseudomonas fluorescens H2 with glass

McEldowney, Sharron

doi:10.1128/aem.60.8.2759-2765.1994

Cited by 17 publications

(23 citation statements)

References 33 publications

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“…Previous research suggested that attachment of Pseudomonas £uorescens H2 to glass surfaces a¡ected subsequent accumulation of Cd 2 [5]. The aim of this study was to determine the in£uence of attachment to glass surfaces on the uptake of heavy metal cations by P. £uorescens H2.…”

Section: Introductionmentioning

confidence: 95%

“…P. £uorescens H2 (wild-type strain) [15] was maintained on glucose minimal medium (GMM) (pH 7) solidi¢ed with 1% (w/v) pure agar, as previously described [5]. Cultures were stored at 4³C.…”

Section: Bacterial Strain and Storage Conditionsmentioning

confidence: 99%

“…The cells were harvested by centrifugation (8000Ug avX , 15 min, 4³C), washed once in 0.2 mM maleate bu¡er and resuspended in maleate bu¡er (pH 7) to 1.5^3.0U10 8 cells ml 31 . The cells were incubated at 25³C for 1 h to ensure identical treatment to attached cells, as previously described [5].…”

Section: Preparation Of Cell Suspensionsmentioning

confidence: 99%

“…The potential e¡ects of heavy metals on attached bacteria are complex and include changes in physiological activity and viability of cells, and changes in density and diversity within bacterial communities [4]. Heavy metal cations have also been shown to in£uence bacterial attachment and detachment interactions [5]. In addition, bacteria attached to surfaces and particulates accumulate heavy metal cations internally and at the cell surface [1,6,7] and, by so doing, may in£uence the distribution of heavy metals between surfaces and the aqueous phase in terrestrial and aquatic systems [6].…”

Section: Introductionmentioning

confidence: 99%

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The impact of surface attachment on cadmium accumulation by Pseudomonas fluorescens H2

McEldowney

2000

FEMS Microbiology Ecology

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Cadmium accumulation by Pseudomonas fluorescens H2 attached to glass surfaces and by cells free in suspension in maleate buffer was compared and showed several major differences. The time to saturation of cells with Cd(2+) was different for attached and free cells, although both showed biphasic uptake of Cd(2+). The accumulation of Cd(2+) by free cells was inhibited by the presence of zinc but remained several orders of magnitude higher than accumulation by attached cells. The presence of Zn(2+), however, did not inhibit Cd(2+) uptake by cells attached to the solid substratum. Cadmium uptake still increased with Cd(2+) concentration for both free and attached cells in the presence of Zn(2+). The accumulation of Cd(2+) by both free and attached cells increased with pH up to pH 11. The presence of a metabolic inhibitor, carbomyl cyanide m-chlorophenyl-hydrazone, reduced the uptake of Cd(2+) by free cells by 40% but reduced uptake by attached cells by only 25%. Approximately 65% of Cd(2+) accumulated by cells free in suspension was associated with the cell wall, 33% was present in the cytoplasm and only 2% was bound to exopolymer. The results suggest that the characteristics of heavy metal accumulation by bacterial cells are substantially affected by attachment to solid surfaces.

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Section: Introductionmentioning

confidence: 95%

Section: Bacterial Strain and Storage Conditionsmentioning

confidence: 99%

Section: Preparation Of Cell Suspensionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The impact of surface attachment on cadmium accumulation by Pseudomonas fluorescens H2

McEldowney

2000

FEMS Microbiology Ecology

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“…Adhesiveness to surfaces was assessed by suspending glass slides vertically in 100 ml DCM at 25°C for 24 h. Attached cells were enumerated microscopically by staining (McEldowney 1994). Colony-forming units in suspension were enumerated as described above and the relative percentage adhesion calculated.…”

Section: Determination Of Adhesion Of Bacteria Isolated From Biofilmsmentioning

confidence: 99%

Assessment of microbial involvement in the elevation of copper levels in drinking water

Dutkiewicz

Fallowfield

1998

Journal of Applied Microbiology

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A study of bacterial populations in metropolitan Adelaide domestic reticulation pipes was conducted to investigate a possible link between copper in drinking water and biofilms. Biofilm densities from cold water copper pipes at 10 sample sites were measured by viable cell counts. The range detected was from <2 × 101 to 3·25 × 107 cfu cm−2. Five isolates were selected for further experiments as they represented a range of responses to solvated copper and relative tendency for adhesion on glass slides. Drinking water supplied to the Adelaide Hills is high in total organic carbon (TOC; 22·57 mg C l−1) and has a negative Langelier Index (LI; −1·16), whereas Adelaide metropolitan water undergoes filtration and has both a lower TOC and LI (10·72 mg C l−1, LI, −0·49). Copper coupons were exposed to biofilm isolates (24 h), washed and resuspended in Adelaide metropolitan and Adelaide Hills water. Copper coupons not exposed to biofilm isolates were suspended in respective waters as a control. After 5 d of incubation, the copper content of Adelaide Hills water (4·71 ± 0·87 mg Cu l−1), in which the copper coupons were suspended, consistently exceeded values obtained in the metropolitan Adelaide water (1·17 ± 0·249 mg Cu l−1). The concentration of copper in the Adelaide Hills water was influenced by the bacterial species forming the biofilm on the coupon, with Agrobacterium sp. producing significantly higher levels of soluble copper than the control. The experiments reported here indicate that the suspended organic carbon, the aggressivity of the water and the biofilm may independently or synergistically increase the dissolution of copper from pipes into drinking water.

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Estimation of growth inhibition by copper and cadmium in heavy metal tolerant actinomycetes

Amoroso¹,

Oliver²,

Castro³

2002

J. Basic Microbiol.

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Effect of cadmium and zinc on attachment and detachment interactions of Pseudomonas fluorescens H2 with glass

Cited by 17 publications

References 33 publications

The impact of surface attachment on cadmium accumulation by Pseudomonas fluorescens H2

The impact of surface attachment on cadmium accumulation by Pseudomonas fluorescens H2

Assessment of microbial involvement in the elevation of copper levels in drinking water

Estimation of growth inhibition by copper and cadmium in heavy metal tolerant actinomycetes

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