Effect of cadmium-feeding on tissue concentrations of elements in germ-free silkworm (Bombyx Mori) larvae and distribution of cadmium in the alimentary canal

Suzuki, Kazuo; Aoki, Yosuke; Nishikawa, Masataka; Masui, Hiroyuki; Matsubara, Fujiyoshi

doi:10.1016/0742-8413(84)90194-4

Cited by 23 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In various insect larvae Cd was found to be accumulated mainly in the gut epithelium Suzuki et al 1984). This was also true for Cd and inorganic Hg in adults of the carabid beetle Pterostichus niger, whereas Zn and rnethyl-Hg were found to be distributed throughout the body .…”

Section: Resultsmentioning

confidence: 63%

Losses of Cd, Hg, and Zn During Metamorphosis in the Beetle Tenebrio Molitor (Coleoptera: Tenebrionidae)

Lindqvist

Block

1997

Bulletin of Environmental Contamination and Toxicology

View full text Add to dashboard Cite

Insects occur in great numbers and are important for the transport of metals between trophic levels in food webs. Therefore, processes influencing metal concentrations in insects are important for the distribution of metals in ecosystems. The metamorphosis in holometabolous insects is of importance for metal concentrations. During this process some organs undergo an extensive breakdown as new organs are built up (Wigglesworth 1974). In the course of this process, concentrations of Cd were found to be altered .The impact of the metamorphosis on concentrations of other metals has been less studied. Nuorteva and Nuorteva (1982) found that Hg concentrations were lower in adult blowflies than in larvae, which suggests that Hg concentrations are altered during metamorphosis. Zn is an essential metal and is subject to homeostatic regulation (Chapman 1982). If no changes in metabolic pathways are needed, the optimal concentration of Zn may remain unchanged.The aim of this study was to measure the loss of Cd during metamorphosis in an insect and to determine whether Hg and Zn undergo a similar type of decrease. Hg was studied in both inorganic and methylated forms in order to reveal how the chemical form of this metal influences its metabolism. Tenebrio molitor was used in this study because adults and larvae share the same diet. Since this study lasts from the larval stage to the adult stage, the type of food is not changed. A shift in diet could influence the metabolism of the metals. MATERIALS AND METHODSThe metals were accumulated during the larval period in Tenebrio molitor. Preultimate instar larvae of T. molitor were kept in darkness at 20°C and 65% R.H.For each treatment, 10 larvae were reared individually in glass boxes and given wheat bran as food. The larvae were also given a piece of potato containing 109 Cd, 203 Hg, Methyl-203 Hg or 65 Zn. The potato pieces, approximately 2*5*10 mm, had been incubated 30 min in a 0.9% NaCl solution containing one of the metals. After the incubation, the potato pieces were dried on a paper towel for 1 hr and thereafter given to the larvae. The potato pieces were replaced by untreated potato after one week.Correspondence to: L. LindqvistThe following chemicals were used: 109 C d C l 2 , specific activity 1.76 mCi/mg, 65 ZnC1 2 , 1.62 mCi/mg, 203 Hg(NO 3 ) 2 , 1.37 mCi/mg; all of which were purchased from Du Pont Scandinavia, Stockholm, Sweden. Methyl-203 Hg was synthesized according to Naganuma et al. (1985).Metal contents were measured in the larvae in vivo during the last larval stage using a gamma-counter (Searle model 1185). Thereafter contents of the metals were monitored every third day throughout metamorphosis and for 27 days following eclosion of adults. RESULTS AND DISCUSSIONContents of all the studied metals declined at pupation when the exuvium was cast off and the pupal stage began. The 109 Cd content of newly eclosed adults was 76% of that of the last instar larvae. For 65 Zn it was 87%, for 203 Hg 65% and for methyl-203 Hg 83% (Fig. 1).In various insect larvae Cd ...

show abstract

Section: Resultsmentioning

confidence: 63%

Losses of Cd, Hg, and Zn During Metamorphosis in the Beetle Tenebrio Molitor (Coleoptera: Tenebrionidae)

Lindqvist

Block

1997

Bulletin of Environmental Contamination and Toxicology

View full text Add to dashboard Cite

show abstract

“…Cd is largely associated with the insect gut in both aquatic (Seidman et aB. H986a;Hare et al 1991; this study) and terrestrial (Aoki et al 1984;Suzuki et al 1984) taxa. The potentially large quantities of trace metals present in the gut could be stored in granules and/or lysssomes which have been observed in both aquatic (Seidman et al 1986b) and terrestrial (Jemtet et al 1977;Tapp and Hockaday 19'97) insects.…”

Section: Cutmentioning

confidence: 67%

Trace Element Distributions in Aquatic Insects: Variations among Genera, Elements, and Lakes

Hare¹,

Tessier²,

Campbell³

1991

Can. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

The distribution patterns of trace element contaminants (As, Cd, Cu, Pb, Zn) among the tissues of freshwater insects (Chironomus, Clinotanypus, Procladius, Hexagenia, Sialis) are both taxon and element specific. In quantity terms (micrograms per animal), the gut is the major organ of Cd accumulation, while most of the As, Cu, Pb, and Zn accumulates in the body (i.e. animal minus gut and other organs). In concentration terms (micrograms per gram), Cd, Cu, and Zn are often highest in the gut, while Pb and As are not. Much of the As, and in some genera the Pb, appears to be bound externally. Respiratory and excretory structures generally contain small proportions of the total trace element burdens, except for Cu. Trace elements are not uniformly distributed along the gut but tend to accumulate either in the mesenteron (midgut) or in the proctodeum (hindgut), depending on the element and insect concerned. The distribution patterns of most of the trace elements vary little among populations of the mayfly Hexagenia from different lakes with the exception of Cd, for which the proportion of the total Cd burden localized in the gut increases markedly with increasing Cd concentrations in the whole nymph.

show abstract

“…In newly hatched adults, the cadmium content dropped to 76% of that of the last instar larvae, which was attributed to the molting process [96]. According to the authors, ingested cadmium was distributed to two different pools: One pool was associated with the gut epithelium cells supported by the result published earlier that cadmium was reported to accumulate mainly in the alimentary canal in silkworms assumedly bound to inducible high molecular weight cadmium-binding proteins [97,98,99]. During metamorphosis, the midgut epithelium is renewed and thus, can be excreted via feces.…”

Section: Heavy Metals and Arsenicmentioning

confidence: 99%

Insects for Food and Feed-Safety Aspects Related to Mycotoxins and Metals

Schrögel

Wätjen

2019

Foods

View full text Add to dashboard Cite

Edible insects as an alternative source of protein are discussed as an important contribution to future feed and food security. However, it has to be ensured that the consumption is non-hazardous. This systematic review summarizes findings concerning contaminations of insects with mycotoxins and heavy metal ions (SciFinder, Pubmed, until 26 June 2019). Both kinds of contaminants were reported to reduce growth performance and increase mortality in insects. There was no evidence for accumulation of various mycotoxins analyzed in distinct insect species. However, further research is necessary due to limitation of data. Since the gut content contributes relevantly to the total body burden of contaminants in insects, a starving period before harvesting is recommended. Contrary, accumulation of heavy metal ions occurred to a varying extent dependent on metal type, insect species, and developmental stage. Examples are the accumulation of cadmium (black soldier fly) and arsenic (yellow mealworm). The reported species-specific accumulation and metabolism patterns of contaminants emphasize the importance to assess potential safety hazards in a case-by-case approach. Subject to regular monitoring of contaminants, the general ban in the European Union to use waste in animal feed should also be questioned regarding insect farming.

show abstract

Effect of cadmium-feeding on tissue concentrations of elements in germ-free silkworm (Bombyx Mori) larvae and distribution of cadmium in the alimentary canal

Cited by 23 publications

References 7 publications

Losses of Cd, Hg, and Zn During Metamorphosis in the Beetle Tenebrio Molitor (Coleoptera: Tenebrionidae)

Losses of Cd, Hg, and Zn During Metamorphosis in the Beetle Tenebrio Molitor (Coleoptera: Tenebrionidae)

Trace Element Distributions in Aquatic Insects: Variations among Genera, Elements, and Lakes

Insects for Food and Feed-Safety Aspects Related to Mycotoxins and Metals

Contact Info

Product

Resources

About