Effect of Cadmium on Activities of Some Enzymes of Glycolysis and Pentose Phosphate Pathway in Pea

Chugh, L.K.; Sawhney, S.

doi:10.1023/a:1002417319599

Cited by 28 publications

(4 citation statements)

References 16 publications

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“…In our study, we observed heavy metal treatments caused chlorosis in rocket plants. A decrease in cadmiuminduced chlorophyll concentration was shown in barley (Stobart et al, 1985), beans (Padmaja et al, 1990), cucumber (Chugh and Sawhney, 1999), corn and wheat (Zhao, 2011). Similarly, it has been reported that Cd toxicity causes chlorosis, leaf curling and stunting (Moreno et al, 1999;Emamverdian et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

Impact of Cadmium and Lead Heavy Metal Stress on Plant Growth and Physiology of Rocket (Eruca sativa L.)

Yıldırım

Ekinci

Turan

et al. 2019

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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This study was conducted to evaluate the influence of cadmium (Cd) (0, 100, 150 and 200 mg kg-1) and lead (Pb) (0, 1000, 1500 and 2000 mg kg-1) on morphological, physiological and biochemical responses of rocket (Eruca sativa L.) in greenhouse conditions. Plant growth, some physiological (membrane permeability, relative water content, stomal conductance, etc.) and biochemical (antioxidant enzyme activity, proline and sucrose content) parameters of rocket plants were altered with Cd and Pb levels. The Cd and Pb content in rocket increased with elevated concentration. Both Cd and Pb stress conditions negatively affected plant growth, photosynthetic activity and chlorophyll content. The negative effect of heavy metals elevated with increased doses of Cd and Pb. The treatment of Cd and Pb significantly elevated antioxidant enzyme activities. Furthermore, the heavy metal stressed plants had more malondialdehyde (MDA), hydrogen peroxide (H2O2), proline and sucrose as compared to control plants. This study indicated that rocket plants developed defense mechanisms by regulating enzyme activity and osmolyte accumulation against heavy metal stress.

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Section: Resultsmentioning

confidence: 99%

Impact of Cadmium and Lead Heavy Metal Stress on Plant Growth and Physiology of Rocket (Eruca sativa L.)

Yıldırım

Ekinci

Turan

et al. 2019

Kahramanmaraş Sütçü İmam Üniversitesi Tarım Ve Doğa Dergisi

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“…This two‐step process also produces reducing equivalents (nicotinamide adenosine dinucleotide phosphate [NADPH]) for cellular anabolic reactions. PPP also generates erythrose‐4‐phosphate which is channeled to the shikimate pathways to produce phenylalanine, which is directed through the phenylpropanoid pathway to produce phenolic phytochemicals (Mann 1978; Chugh and Sawhney 1999; Shetty et al. 2003; Shetty and Wahlqvist 2004; Vattem et al.…”

Section: Introductionmentioning

confidence: 99%

Apple Postharvest Preservation Is Linked to Phenolic Content and Superoxide Dismutase Activity

Adyanthaya

Kwon

Apostolidis

et al. 2009

Journal of Food Biochemistry

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The postharvest preservation of apples indicated that well‐preserved varieties of apples had increased superoxide dismutase (SOD) activity initially, and the activity declined during later storage as apples deteriorated. The increased SOD activity linked to better preservation correlated with higher phenolic content and free‐radical scavenging‐linked antioxidant activity. Well‐preserved varieties were able to maintain a more stable pentose phosphate pathway (measured by the activity of glucose‐6‐phosphate dehydrogenase) throughout the storage period. Proline content increased with proline dehydrogenase (PDH) activity in the initial storage period, indicating proline catabolism supporting potential adenosine 5′‐triphosphate (ATP) synthesis. During later storage, succinate dehydrogenase activity increased, while PDH activity declined indicating a shift to tricarboxylic acid cycle and likely nicotinamide adenine dinucleotide hydrogen (NADH) generation for ATP synthesis. This shift, coupled with the declining SOD activity, coincides with rapid deterioration. The guaiacol peroxidase activity generally declined in late stages, indicating postharvest deterioration. PRACTICAL APPLICATIONS Understanding the phenolic‐linked antioxidant response during postharvest preservation of apples has implications for understanding critical control points for preservation and determination of better biochemical markers associated with preservation and long‐term storage. These insights would also help elucidate markers such as high phenolic content and antioxidant enzyme response pathways that would allow for selection and breeding of varieties that would preserve better. Higher phenolic content not only improves the postharvest storage of apples but have also shown human health benefits.

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“…Alcohol dehydrogenase is thought to sequester cadmium ions by displacing bound zinc (Suzuki et al ., 1990) and is regarded as an initial buffering response prior to the induction of metallothionein (Sunaga et al ., 1988). Previous work with rat liver microsomes and peas ( Pisum sativum ) has documented a decrease in the activity of alcohol dehydrogenase (Waku, 1984; Chugh and Sawhney, 1999) following cadmium exposure while Lee et al . (2006) found that the midge ( Chironomus riparius ) up‐regulated this protein after exposure to cadmium.…”

Section: Discussionmentioning

confidence: 97%

Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants

Sanchez

Carter

Hammers

et al. 2011

J of Applied Toxicology

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Microarrays enable gene transcript expression changes in near-whole genomes to be assessed in response to environmental stimuli. We utilized oligonucleotide microarrays and subsequent gene set enrichment analysis (GSEA) to assess patterns of gene expression changes in male largemouth bass (Micropterus salmoides) hepatic tissues after a 96 h exposure to common environmental contaminants. Fish were exposed to atrazine, cadmium chloride, PCB 126, phenanthrene and toxaphene via intraperitoneal injection with target body burdens of 3.0, 0.00067, 2.5, 50 and 100 µg g(-1), respectively. This was conducted in an effort to identify potential biomarkers of exposure. The expressions of 4, 126, 118, 137 and 58 mRNA transcripts were significantly (P ≤ 0.001, fold change ≥2×) affected by exposure to atrazine, cadmium chloride, PCB 126, phenanthrene and toxaphene exposures, respectively. GSEA revealed that none, four, five, five and three biological function gene ontology categories were significantly influenced by exposure to these chemicals, respectively. We observed that cadmium chloride elicited ethanol metabolism responses, and along with PCB 126 and phenanthrene affected transcripts associated with protein biosynthesis. PCB 126, phenanthrene and toxaphene also influenced one-carbon compound metabolism while PCB 126 and phenanthrene affected mRNA transcription and mRNA export from the nucleus and may have induced an antiestrogenic response. Atrazine was found to alter the expression of few hepatic transcripts. This work has highlighted several biological processes of interest that may be helpful in the development of gene transcript biomarkers of chemical exposure in fish.

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Effect of Cadmium on Activities of Some Enzymes of Glycolysis and Pentose Phosphate Pathway in Pea

Cited by 28 publications

References 16 publications

Impact of Cadmium and Lead Heavy Metal Stress on Plant Growth and Physiology of Rocket (Eruca sativa L.)

Impact of Cadmium and Lead Heavy Metal Stress on Plant Growth and Physiology of Rocket (Eruca sativa L.)

Apple Postharvest Preservation Is Linked to Phenolic Content and Superoxide Dismutase Activity

Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants

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