Effect of Cadmium on<i>Arabidopsis Thaliana</i>Mutants Tolerant to Oxidative Stress

Radeva, V.; Petrov, Veselin; Minkov, Ivan; Toneva, Valentina; Gechev, Tsanko

doi:10.1080/13102818.2010.10817823

Cited by 4 publications

(4 citation statements)

References 58 publications

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“…Moreover, the appearance of a polypeptide with a molecular weight 18 kDa in seedlings of treated pea may be calcium binding protein It is clearly shown that enzymatic antioxidants (peroxidase, polyphenyl oxidase and catalase) activities in pea seedlings were gradually increased by increasing the concentrations of cadmium. These results are in agreement with that reported by Radeva et al (2010). Peroxidase which participates in lignin biosynthesis might build up a physical barrier against toxic heavy metals.…”

Section: Resultssupporting

confidence: 83%

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

El-Beltagi

Mohamed

2013

Not Bot Hort Agrobot Cluj

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The present investigation was carried out to study the role of calcium chloride in enhancing tolerance and reducing cadmium toxicity in pea seedlings. Some treatment with 1 and 5 mM CaCl 2 mitigated cadmium stress by increasing antioxidant enzyme activities: catalase (CAT), peroxidase (POD) and polyphenol oxidase (PPO), as well as by elevating contents of ascorbic acid (ASA), tocopherol and carotenoids. On the other hand, total carbohydrate and total soluble proteins decreased with increasing cadmium concentrations in comparison with control plants. However, total phenol, total free amino acids, proline and lipid peroxidation increased with increasing concentrations of cadmium acetate. Electrophoretic studies of protein revealed that cadmium treatments alone or in combination with calcium chloride were associated with the disappearance of some bands or appearance of new bands in pea seedlings. Electrophoretic studies of α-esterase, β-esterase and acid phosphatase isozymes showed wide variations in their intensities and densities.

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Section: Resultssupporting

confidence: 83%

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

El-Beltagi

Mohamed

2013

Not Bot Hort Agrobot Cluj

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“…To investigate the effects of Cd stress on root growth, 4-day-old seedlings cultivated in control agar plates were transferred to freshly prepared medium supplemented by various concentration of CdCl 2 (25, 50, 75, and 100 μM) as indicated ( Cao et al, 2009 ; Radeva et al, 2010 ) for 2 and 4 days. Preliminary experiments with seedling grown with various CdCl 2 concentrations showed that 75 μM CdCl 2 decreased root growth by about 40% and so this concentration was used to compare the effects of Cd stress between the wild type and the ethylene-insensitive mutants.…”

Section: Methodsmentioning

confidence: 99%

Ethylene Improves Root System Development under Cadmium Stress by Modulating Superoxide Anion Concentration in Arabidopsis thaliana

et al. 2017

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This work aims at identifying the effects of ethylene on the response of Arabidopsis thaliana root system to cadmium chloride (CdCl2) stress. Two ethylene-insensitive mutants, ein2-5 and ein3-1eil1-1, were subjected to (25, 50, 75, and 100 μM) CdCl2 concentrations, from which 75 μM concentration decreased root growth by 40% compared with wild type Col-0 as a control. Ethylene biosynthesis increased in response to CdCl2 treatment. The length of primary root and root tip in ein2-5 and ein3-1eil1-1 decreased compared with wild type after CdCl2 treatment, suggesting that ethylene play a role in root system response to Cd stress. The superoxide concentration in roots of ein2-5 and ein3-1eil1-1 was greater than in wild type seedlings under Cd stress. Application of exogenous 1-aminocyclopropane-1-carboxylic acid (ACC) (a precursor of ethylene biosynthesis) in different concentrations (0.01, 0.05 and 0.5 μM) decreased superoxide accumulation in Col-0 root tips and increased the activities of superoxide dismutase (SOD) isoenzymes under Cd stress. This result was reversed with 5 μM of aminoisobutyric acid AIB (an inhibitor of ethylene biosynthesis). Moreover, it was accompanied by increase in lateral roots number and root hairs length, indicating the essential role of ethylene in modulating root system development by controlling superoxide accumulation through SOD isoenzymes activities. The suppressed Cd-induced superoxide accumulation in wild type plants decreased the occurrence of cells death while programmed cell death (PCD) was initiated in the root tip zone, altering root morphogenesis (decreased primary root length, more lateral roots and root hairs) to minimize the damage caused by Cd stress, whereas this response was absent in the ein2-5 and ein3-1eil1-1 seedlings. Hence, ethylene has a role in modulating root morphogenesis during CdCl2 stress in A. thaliana by increasing the activity of SOD isoenzymes to control superoxide accumulation.

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“…The relationship between Cd and oxidative stresses manifests also in experiments with Arabidopsis mutants which are characterized by tolerance to both of them. Such is the case for plants overexpressing an aldehyde dehydrogenase ( ALDH3 ; Sunkar et al, 2003 ), mutants of a MAP-kinase ( mpk6 ; Jin et al, 2013 ) and the atr lines which are less sensitive to Cd- and paraquat-induced oxidative stress ( Radeva et al, 2010 ).…”

Section: Ros-induced Pcd Against Particular Abiotic Stimulimentioning

confidence: 99%

ROS-mediated abiotic stress-induced programmed cell death in plants

et al. 2015

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During the course of their ontogenesis plants are continuously exposed to a large variety of abiotic stress factors which can damage tissues and jeopardize the survival of the organism unless properly countered. While animals can simply escape and thus evade stressors, plants as sessile organisms have developed complex strategies to withstand them. When the intensity of a detrimental factor is high, one of the defense programs employed by plants is the induction of programmed cell death (PCD). This is an active, genetically controlled process which is initiated to isolate and remove damaged tissues thereby ensuring the survival of the organism. The mechanism of PCD induction usually includes an increase in the levels of reactive oxygen species (ROS) which are utilized as mediators of the stress signal. Abiotic stress-induced PCD is not only a process of fundamental biological importance, but also of considerable interest to agricultural practice as it has the potential to significantly influence crop yield. Therefore, numerous scientific enterprises have focused on elucidating the mechanisms leading to and controlling PCD in response to adverse conditions in plants. This knowledge may help develop novel strategies to obtain more resilient crop varieties with improved tolerance and enhanced productivity. The aim of the present review is to summarize the recent advances in research on ROS-induced PCD related to abiotic stress and the role of the organelles in the process.

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Effect of Cadmium onArabidopsis ThalianaMutants Tolerant to Oxidative Stress

Abstract: ABSTRACT

Cited by 4 publications

References 58 publications

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

Alleviation of Cadmium Toxicity in Pisum sativum L. Seedlings by Calcium Chloride

Ethylene Improves Root System Development under Cadmium Stress by Modulating Superoxide Anion Concentration in Arabidopsis thaliana

ROS-mediated abiotic stress-induced programmed cell death in plants

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