Effect of cadmium stress on inductive enzymatic and nonenzymatic responses of ROS and sugar metabolism in multiple shoot cultures of Ashwagandha (Withania somnifera Dunal)

Mishra, Bhawana; Sangwan, Rajender S.; Mishra, Saurabh; Jadaun, Jyoti Singh; Sabir, Farzana; Sangwan, Neelam S.

doi:10.1007/s00709-014-0613-4

Cited by 89 publications

(57 citation statements)

References 37 publications

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“…These environmental stresses, irrespective of source, trigger numerous plant responses, ranging from altered gene expression and cellular metabolism to changes in plant biochemistry, growth and development (Miller et al, 2008;Jaleel, 2009Ahmad et al, 2010Huang et al, 2012;Mishra et al, 2014). Cold stress, one of the most harmful abiotic stresses affects growth, development and geographic distribution of plants.…”

Section: Introductionmentioning

confidence: 99%

“…Plants have evolved several enzymatic and non-enzymatic mechanisms to provide protection against the toxic effects of ROS and to maintain cellular redox homeostasis (Ahmad et al, 2012;Jaleel et al, 2007Jaleel et al, , 2009; Kaur et al, 2012;Sabir et al, 2012;Mishra et al, 2014). ROS scavenging enzymes include catalase (CAT), ascorbate peroxidase (APX), superoxide dismutase (SOD), and glutathione reductase (GR; Noctor and Foyer, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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Withania somnifera (L.) Dunal (Indian ginseng) is a high value medicinal plant. It synthesizes a large array of biologically active withanolides. In this study, two month old seedlings of AGB002 (wild genotype) and AGB025 (cultivated genotype) of W. somnifera were subjected to cold stress (4 • C) under controlled envi-ronment. Plants were analyzed for three medicinally important secondary metabolites (withanolide A, withanone and withaferin A), lipid peroxidation (MDA), cell injury, superoxide radical (O 2 •− ) accumula-tion and anti-oxidative enzymes activities such as superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR). Increases in the titers of superoxide anion and MDA were observed from day 1 to day 7 in both genotypes, although the increase on the first day of exposure was significantly higher. Enzymatic activities of SOD, CAT, APX and GR also showed an increasing trend in both genotypes and reached a maximum on day 7 of the cold temperature exposure; however, this increase was higher in AGB002 than AGB025. Withanolide A (WS-1) in the roots of both genotypes sig-nificantly decreased on the first day of cold exposure and then showed a recovery until day 7. WS-1 was not detected in the leaves of either genotype. Withanone (WS-2) content in the leaves also decreased towards the end of the cold period in both genotypes. Cold stress also elicited the accumulation of WS-2 in AGB025, but was not detectable in control seedlings. At maturity, WS-2 was also detected in control plants. Furthermore, a significant increase in the leaf withaferin A (WS-3) content was recorded from day 1 to day 7 of the cold exposure in both the genotypes, suggesting the possible involvement of with-anolides in cold-protection. AGB002 showed comparatively higher accumulation of antioxidant enzymes and selected marker withanolides than AGB025, indicating that AGB002 is better adapted to cold than AGB025. It could be inferred from these observations that cold stress induces bioactive withanolide accu-mulation in W. somnifera as a mechanism for scavenging reactive oxygen species (ROS). These studies also provide an impetus for enhancing the withanolide accumulation in W. somnifera using controlled environment technology.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Mir

Khazir

et al. 2015

Industrial Crops and Products

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“…7A and B). Cd을 처리하지 않은 무처리에 있어, P시용 유무에 따라 뿌리와 잎의 두 조직 모두에서 통계적인 차이를 나타내지 않았지만 Cd + P처리에서는 AsA 함량이 크게 증가됨을 관찰할 수 있었다 ( (Arshad et al, 2016;Mishra et al, 2014). Rice seedlings at the four-leaf stage were cultivated in a hydroponics containing 30 µM Cd, with or without 6 mM P, for 14 days.…”

Section: 활성산소종과 지질과산화 변화 카드뮴독성으로 인하여 유도되는 산화적 스트레스 정도를 분석하기 위하여unclassified

“…비료의 3요소 중의 하나인 인과 중금속인 비소의 길항작용에 관한 연관성 연구는 여러 연구보고들을 통하여 확실한 정설로 자리매김을 하고 있는 실 정임에도 불구하고 (Brachhage et al, 2014;Gomes et al, 2014), 카드뮴과 인의 상호연관성 연구는 일부 식물에서의 연구를 제외하고 아직도 미진한 상태이므로 깊이있는 연구가 필요할 것으로 판단된다 (Arshad et al, 2016). 인은 식 물의 주된 에너지원인 아데노신 삼인산 (adenosine triphosphate, ATP) 생성과 여러 효소반응을 촉진하여 Cd의 독성 에 의해 발생되는 활성산소종 소거에 관여할 뿐만 아니라 식물의 생장촉진에도 효과를 나타낸다 (Jalil et al, 1994;Mishra et al, 2014 (Jung et al, 2015;Kamachi et al, 1991). Jung et al (2015) …”

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Effect of Phosphorus on the Cadmium Transfer and ROS-scavenging Capacity of Rice Seedlings

Jung¹,

Chae²,

Maria³

et al. 2017

Korean J. Soil. Sci. Fert.

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Environmental toxicity due to cadmium (Cd) pollution in croplands causes critical problems worldwide. Rice (Oryza sativa L.) is an important crop in Asia, including South Korea, and numerous studies have evaluated the relationship between Cd and antioxidants to alleviate Cd uptake from the soil into plants. However, information about the relationship between phosphorus (P) and antioxidants in rice seedlings is still limited with regard to Cd phytotoxicity. We therefore investigated the physiological responses of rice (Oryza sativa L. cv 'Dongjin') seedlings to Cd toxicity and the effect of P application on reactive oxygen species (ROS) and antioxidant changes. The exposure of rice seedlings to 30 µM Cd inhibited plant growth; increased the contents of superoxide, hydrogen peroxide, and malondialdehyde; and induced Cd uptake by the roots and leaves. Application of P to Cd-exposed seedlings decreased Cd-induced oxidative stress by enhancing the capacity of ascorbate (AsA) production and ROS-scavenging, and decreased Cd transfer from the roots to the leaves. These results suggest that P application alleviated Cd-induced growth inhibition and oxidative damage by restricting Cd translocation from the roots to the leaves and maintaining sufficient levels of AsA.Keywords: Antioxidant, Cadmium, Phosphorus, Reactive oxygen species (ROS), Rice ) in roots (A, C) and leaves (B, D) of rice plants grown in the 30 µM Cd-treated hydroponics with or without 6 mM phosphorus (P) application at the four-leaf stage after 14 days.Ⓒ The Korean Society of Soil Science and Fertilizer. This is an Open Access article distributed under the terms of the Creative Commons Attribution NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. Science and Fertilizer Vol. 50, No. 4, 2017 Introduction 세계적인 산업화 및 도시화는 농경지, 농업용수 그리고 생활용수 등의 오염수준을 급속히 증가시키고 있다 (Galiulin et al., 2001;Kirkham, 2006). 카드뮴 오염은 환경적으로 부정적인 영향을 끼칠 뿐만 아니라 먹이사슬의 최 정상에 위치한 인간의 건강을 위협하는 치명적인 위해요소로 간주되고 있다 (Jan et al., 2015;Nagajyoti et al., 2010). • Korean Journal of Soil카드뮴은 전반적인 식물생장에 부정적으로 작용하며 세포분열을 방해하여 뿌리의 신장을 억제할 뿐만 아니라 보조인 자로 필수금속이온 (Cu, Zn, Mn, Fe 등)을 포함한 단백질들의 금속이온을 치환함으로써 펜톤반응 (fenton reaction)활성산소종 (reactive oxygen species, ROS)을 발생시켜 세포 내에서 지질과산화작용을 야기함으로써 세포막을 파괴 하고 최종적으로 세포를 사멸시킨다 (Barceló et al., 1988;Jung et al., 2015Jung et al., , 2016Lagriffoul et al., 1998;. 특히 카드뮴에 의해 발생된 산화적 스트레스는 활성산소종의 세포 내 축 적으로 인하여 탄수화물, 단백질, 지질 및 핵산의 생합성에 부정적인 영향을 미치고 산화 ․ 환원 항상성을 교란하여 정 상적인 생체 기능을 저해한다 (Gayomba et al., 2013;Singh and Agrawal, 2010;Singh et al., 2006, Xu et al., 2014. (Fig. 2). 이상의 결과에 근거하여, 벼 뿌리 및 잎으로의 카드뮴전이에 P의 시용효과가 확인되었다. 뿌리에서 16% 감소를 나타냈고, 특히 지상 부인 잎으로의 전이를 44% 감소시킴으로써 전반적인 카드뮴독성을 완화시키는 것으로 판단된다 (Jiang et al., 2007;Mishra et al., 2014;Sajwan et al., 2002). Table 1. Effects of the application of phosphorus (P) on the plant growth characteristics in rice plants grown in the 30 µM cadmium (Cd)-treated hydroponics ...

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Lead Toxicity and Tolerance in Plants

Hassan,

Chafik,

Lebrun

et al. 2023

Heavy Metal Toxicity and Tolerance in Plants

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Effect of cadmium stress on inductive enzymatic and nonenzymatic responses of ROS and sugar metabolism in multiple shoot cultures of Ashwagandha (Withania somnifera Dunal)

Cited by 89 publications

References 37 publications

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Cold stress affects antioxidative response and accumulation of medicinally important withanolides in Withania somnifera (L.) Dunal

Effect of Phosphorus on the Cadmium Transfer and ROS-scavenging Capacity of Rice Seedlings

Lead Toxicity and Tolerance in Plants

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