Effect of caffeic acid on tert-butyl hydroperoxide-induced oxidative stress in U937

Nardini, Mirella; Pisu, Paola; Gentili, Vincenzo; Natella, Fausta; Di, Maurizio; Piccolella, FeliceEnza; Scaccini, Cristina

doi:10.1016/s0891-5849(98)00180-4

Cited by 98 publications

(61 citation statements)

References 53 publications

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“…Normally the phenolic compounds act by scavenging free radical and quenching the lipid peroxidation [28]. As presented here caffeic acid has the ability to scavenge the free radicals [29] and attenuate the lipid peroxidation as indicated by increased levels of enzymic antioxidants in the liver [30].…”

Section: Resultsmentioning

confidence: 70%

Antioxidant Effect of Caffeic Acid on Oxytetracycline Induced Lipid Peroxidation in Albino Rats

Rajendran

Subash

2010

Ind J Clin Biochem

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Caffeic acid is a well-known phenolic compound widely present in plant kingdom. The aim of this study was to investigate the possible protective effect of caffeic acid (CA) against oxytetracycline (OXT) induced hepatotoxicity in male Albino Wistar rats. A total of 30 rats weighing 150-170 g were randomly divided into five groups of six rats in each group. Oral administration of OXT (200 mg/kg body weight/day) for 15 days produced hepatic damage as manifested by a significant increase in serum hepatic markers namely aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), bilirubin and increased plasma and hepatic lipid peroxidation indices (TBARS and hydroperoxide). The present finding shows that the levels of enzymatic antioxidants namely superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were significantly decreased in OXT intoxicated rats. Upon oral administration of caffeic acid (40 mg/kg body weight/day) there were decreased hepatic marker activities, bilirubin and lipid peroxidation and increased enzymatic antioxidants in OXT ? Caffeic acid group compared to Normal ? OXT group(P \ 0.05). Our study suggests that caffeic acid has antioxidant property and hepatoprotective ability against OXT induced toxicity.

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Section: Resultsmentioning

confidence: 70%

Antioxidant Effect of Caffeic Acid on Oxytetracycline Induced Lipid Peroxidation in Albino Rats

Rajendran

Subash

2010

Ind J Clin Biochem

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“…44,45) Previous studies have shown that CA is effective in protecting U937 against tert-butyl hydroperoxide-induced oxidative damage both in vivo and in vitro. 46) Therefore, the inhibitory effect of CA on the TNF-a-induced activation of NF-kB might be due to its antioxidant properties. This finding, in conjunction with a report showing that CA prevents hyperglycemia and atherogenesis, suggests that CA can play an important role in preventing atherosclerosis.…”

Section: Discussionmentioning

confidence: 99%

Effect of Caffeic Acid on Tumor Necrosis Factor-Alpha-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Moon

Lee

Kim

et al. 2009

Biological & Pharmaceutical Bulletin

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Recruitment of specific leukocyte subpopulations at the site of inflammation requires a series of cell adhesion molecules (CAMs)-mediated interactions. The major CAMs, viz., intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin are expressed on endothelium in response to various cytokines. Caffeic acid (CA), a natural phenolic compound from herbs and other sources, has been shown to prevent cardiovascular diseases. We investigated the effect of CA on the expression of CAMs by human umbilical vein endothelial cells (HUVECs) stimulated with tumor necrosis factor (TNF-a a). Adhesion of monocytes to CAtreated HUVECs was evaluated by co-culture experiments using 2,7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethylester (BCECF-AM) labeling of U937 cells. The expression of adhesion and chemoattractant molecules was evaluated by Western blot and reverse transcription-polymerase chain reaction (RT-PCR), respectively. CA significantly inhibited the TNF-a a-induced increase in U937 monocyte adhesion to HUVECs as well as decreased the protein and mRNA expression levels of CAMs on HUVECs. CA also inhibited the mRNA expression of monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8). The involvement of nuclear factor (NF)-k kB in the transcriptional control of CAMs protein was assessed by degradation of inhibitory (I)k kB and nuclear translocation of NF-k kB using Western blotting and immunofluorescence staining. CA attenuated TNF-a ainduced Ik kB degradation and NF-k kB translocation from cytosol to the nucleus. In conclusion, TNF-a a-induced NF-k kB-DNA complex formation was inhibited by CA. CA reduced TNF-a a-induced endothelial adhesiveness to HUVECs by inhibiting transcription factor activation, and CAMs expression suggesting its potential role in atherosclerosis diseases.

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“…The same study also found that CAPE could rescue C57BL/6 mice from lethal LPS-induced septic shock while their serum TNF- and IL-1 also decreased [15]. Antioxidant activity of caffeic acid was also showed in t-butyl hydroperoxide-induced oxidative stress in U937 human monocytic cells by blocking glutathione depletion and inhibiting lipid peroxidation [16]. These data supported that chlorogenic and caffeic acids in this food extract may play a role to attenuate LPS-induced enhanced TNF-, COX-2 and iNOS expression by blocking phosphorylation of p38 and Erk1/2 and IB- resulting from reduction of ROS in LPS-activated RAW264.7 cells in the present study.…”

Section: Discussionmentioning

confidence: 75%

“…Chlorogenic acid-rich coffee has been recently demonstrated to induce the Nrf2 (nuclear factor-erythroid 2-related factor-2)/antioxidant-response element (ARE) detoxifying pathway in vitro and in vivo [13]. In addition, humans consuming instant coffee at 800 ml/day for 5 days decreased oxidative stress markers 8-isoprostaglandin F2α by 15.3% and 3-nitrotyrosine by 16.1% in urine [14]. Caffeic acid phenethyl ester (CAPE) has been shown to exhibit anti-inflammatory activity in vitro and in vivo [15].…”

Section: Discussionmentioning

confidence: 99%

Stir-Fry Chicken with Green Curry Suppresses Inflammatory Gene Expression by Lipopolysaccharide-Induced Murine Macrophages

Charoenkiatkul¹,

Muangnoi²,

Chingsuwanrote³

et al. 2011

FNS

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Effect of caffeic acid on tert-butyl hydroperoxide-induced oxidative stress in U937

Cited by 98 publications

References 53 publications

Antioxidant Effect of Caffeic Acid on Oxytetracycline Induced Lipid Peroxidation in Albino Rats

Antioxidant Effect of Caffeic Acid on Oxytetracycline Induced Lipid Peroxidation in Albino Rats

Effect of Caffeic Acid on Tumor Necrosis Factor-Alpha-Induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells

Stir-Fry Chicken with Green Curry Suppresses Inflammatory Gene Expression by Lipopolysaccharide-Induced Murine Macrophages

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