Effect of caffeine-loaded silver nanoparticles on minerals concentration and antibacterial activity in rats

Abstract

Background: Caffeine boosts metabolism and the neurological system. When extreme weakness or sleepiness occurs, it is used recreationally and medicinally to reduce physical and mental fatigue. Caffeine initially stimulates the central nervous system, increasing intellect, speed, accuracy, focus, and coordination. Objective: To evaluate how caffeine nanoparticles affect potassium, calcium, zinc, and magnesium levels, in addition, the anti-bacterial activity of the samples has been employed. Methods: Eighteen male albino rats were divided into three separate groups. The first group (G1) was made up of six animals that served as a control group. The second group (G2) was made up of six animals that were given caffeine, and the third group (G3) was made up of six animals that were given silver nanoparticles from a caffeine solution. The particle size and structural morphology of Caffeine and silver nanoparticles were analyzed using Brookhaven Instruments Corp., XRD and SEM respectively. Results: The structural results showed after addition, caffeine was tube-shaped and silver was spherical granular. Caffeine has more silver nanoparticles than caffeine solution. Caffeine solution affects potassium, calcium, zinc, and magnesium levels. Additionally, the solution has antibacterial activity against the following bacteria: Staphylococcus aureus (Gram +ve), E. coli (Gram –ve), and Pseudomonas aeruginosa (Gram-ve), but it has no effect against yeast (Candida albicans). Conclusions: To determine the biological effects of caffeine-synthesized Ag NPs on zinc, magnesium, calcium, and potassium levels in male albino mice's serum, this is antibacterial against staphylococcus.

An integrated investigation into the antibacterial and antioxidant properties of propolis against Escherichia coli cect 515: A dual in vitro and in silico analysis

“…Overall, higher propolis extract concentrations had larger inhibition zones, while antibiotics, especially gentamicin, were consistently effective. The antibacterial activity observed in our findings surpasses that reported in the study conducted by Rasheed et al (2023), regarding the antibacterial effects of propolis against S. aureus and E. coli. A study by Sanchez-Moreno et al (1998) found that a concentration of 5.48 mg/ml had moderate zone inhibition ranging from 13.0 ± 0.09 to 15.0 ± 0.11 mm against these two bacteria.…”

Synthesis, characterization, cyclic voltammetry, and molecular docking studies of the antioxidant activities of superoxide anion radicals towards meso-tetramethophenyl-porphyrin and meso-tetrabiphenyl-porphyrin

“…The enzymatic reaction is represented by equation ( 9): 2GHS + NAPD l ↔ GSSG + NAPDH + H l (9) Inhibition of glutathione reductase results in a decrease in the level of reduced glutathione (GSH) and an increase in the level of glutathione disulfide (GSSG). Nicotinamide adenine dinucleotide phosphate (NADPH) is crucial for regenerating glutathione, and this process is essential for resisting oxidative stress and maintaining intracellular pH Rasheed et al, 2023). Therefore, studying the inhibition of glutathione reductase is a valuable approach for identifying potential antioxidants.…”

“…In silico ADME study was carried out to predict the adverse metabolic effects of oral administration of TPPH o-methyl and TbPPH as drug candidate, as well as their half-life in the organism and excretion route (Silvino et al, 2016). Cytochrome P450 isoenzymes (CYP450) are oxidases that interact with drugs in order to decrease their plasma concentration and reduce the risks of toxicity by metabolic activation Rasheed et al, 2023), as well as making them more water soluble for elimination (Eitrich et al, 2007;LANEZ et al, 2023). Thus, a drug candidate should not inhibit cytochrome CYP450 isoenzymes because inhibition may increase the plasma concentration (Zahno et al, 2011;Terkeltaub et al, 2011).…”