Effect of calcium and mineral waters on oleic-acid uptake by isolated hamster enterocytes

Goré, J.; Hoinard, Claude; Antoine, Jean Michel; Couet, Charles

doi:10.1051/rnd:19940505

Cited by 3 publications

(2 citation statements)

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“…Also while inclusion of EGTA moderately decreased the susceptibility of erythrocytes to the BAMLET-induced lysis, indicating a role of Ca 2+ , inclusion of CaCl 2 in the medium was protective against hemolysis. Ca 2+ release OA from BAMLET [19], form insoluble salt and lower the availability of OA for interaction with the membrane [44]. The sensitivity of soluble and Sepharose-bound BAMLET-induced hemolysis to the inhibitors suggests similarities in their mechanism of action.…”

Section: Resultsmentioning

confidence: 99%

Oleic Acid May Be the Key Contributor in the BAMLET-Induced Erythrocyte Hemolysis and Tumoricidal Action

et al. 2013

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A chance discovery of the tumoricidal action of a human milk fraction led to the characterization of the active component as oleic acid complex of the α-lactalbumin, which was given the acronym HAMLET. We report in this study that the oleic acid complex of bovine α-lactalbumin (BAMLET) is hemolytic to human erythrocytes as well as to those derived from some other mammals. Indirect immunofluorescence analysis suggested binding of BAMLET to erythrocytes prior to induction of hemolysis. Free OA was hemolytic albeit at higher concentrations, while sodium oleate caused hemolysis at far lower concentrations. Amiloride and BaCl2 offered protection against BAMLET-induced hemolysis suggesting the involvement of a cation leak channel in the process. BAMLET coupled to CNBr-activated Sepharose was not only hemolytic but also tumoricidal to Jurkat and MCF-7 cells in culture. The Sepharose-linked preparation was however not toxic to non-cancerous peritoneal macrophages and primary adipocytes. The tumoricidal action was studied using the MTT-assay while apoptosis induction measured by the annexin V-propidium iodide assay. Repeated incubation of the immobilized BAMLET with erythrocytes depleted oleic acid and decreased the hemolytic activity of the complex. Incubation of MCF-7 and Jurkat cells with OA, soluble or immobilized BAMLET resulted in increase in the uptake of Lyso Tracker Red and Nile red by the cells. The data presented support the contention that oleic acid plays the key role, both in BAMLET-induced hemolysis and tumoricidal action.

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Section: Resultsmentioning

confidence: 99%

Oleic Acid May Be the Key Contributor in the BAMLET-Induced Erythrocyte Hemolysis and Tumoricidal Action

et al. 2013

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“…Indépendamment de la formation de savons insolubles, des essais in vitro sur un modèle d'entérocytes isolés ont montré que l'absorption de l'acide oléique était diminuée après addition de calcium au milieu d'incubation, ce qui est expliqué par un effet direct sur le transport membranaire de l'acide gras (Gore et al, 1994).…”

Section: Résuméunclassified

Interactions digestives et métaboliques entre lipides et calcium

Guéguen¹,

Pointillart²

2008

Sci. Aliments

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Digestive and metabolic interactions between lipids and calciumDietary saturated fats do not significantly decrease intestinal calcium absorption. This explains the good bioavailability of the calcium from milk and dairy products, although these provide large amounts of long chain saturated fatty acids. Conversely, high calcium intakes markedly reduce lipid absorption due to the formation of insoluble calcium soaps, such as calcium stearate or palmitate, depending on the glyceride structure of the ingested lipids. The calcium absorption mainly occurs in the upper part (duodenum) of the digestive tract, whereas the insoluble calcium soaps are formed with the remaining unabsorbed calcium in the distal parts of the small intestine. This explains the apparent contradiction between the two opposite effects, i.e. the calciumrelated decrease of lipid absorption and the unchanged Ca absorption by lipids. The polyinsaturated fatty acids and their by-products seem to enhance active calcium absorption and bone mineralization. But, some discrepancies are observed concerning the effects of arachidonic acid and conjugated linoleic acid (CLA) upon bone remodeling. Some epidemiological observations and a few experiments suggest an inverse relationship between high dietary intake of calcium or of dairy products, and weight gain and/or obesity. According to mainly one group of investigators, the weight loss might be explained by a modulation of adipocyte functions by high calcium. However, this needs to be confirmed by further well-controlled clinical studies before practical applications. Nevertheless, it is well established that high dietary calcium, by diminishing saturated lipid absorption and favourably modifying serum lipid profile, may have protective effects upon cardiovascular diseases.

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